公开(公告)号：US20130130971A1

公开(公告)日：2013-05-23

申请号：US13637845

申请日：2011-04-01

申请人： Celia Berta Fernandez Ortega ,  Anna Caridys Ramírez Suárez ,  Dionne Casillas Casanova ,  Taimi Emelia Paneque Guerrero ,  Raimundo Ubieta Gómez ,  Marta Dubed Echevarria ,  Leonor Margarita Navea Leyva ,  Lila Rosa Castellanos Serra ,  Carlos Antonio Duarte Cano ,  Viviana Falcón Cama ,  Osvaldo Reyes Acosta

发明人： Celia Berta Fernandez Ortega ,  Anna Caridys Ramírez Suárez ,  Dionne Casillas Casanova ,  Taimi Emelia Paneque Guerrero ,  Raimundo Ubieta Gómez ,  Marta Dubed Echevarria ,  Leonor Margarita Navea Leyva ,  Lila Rosa Castellanos Serra ,  Carlos Antonio Duarte Cano ,  Viviana Falcón Cama ,  Osvaldo Reyes Acosta

IPC分类号： A61K38/10 ,  A61K31/713 ,  A61K31/7105 ,  A61K38/17 ,  A61K45/06 ,  C12N5/0783 ,  A61K31/19

CPC分类号： A61K38/10 ,  A61K31/19 ,  A61K31/5575 ,  A61K31/7105 ,  A61K31/713 ,  A61K38/1709 ,  A61K38/1748 ,  A61K45/06 ,  C07K14/47 ,  C07K14/4741 ,  C12N5/0636 ,  C12N15/113 ,  C12N15/1131 ,  C12N15/1132 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2320/31

摘要： The present invention describes a method to inhibit replication of the human immunodeficiency virus (HIV) by negatively modulating or altering the cytoskeleton, more precisely the proteins forming the intermediate cytoskeletal filaments, wherein the said proteins are vimentin and/or keratin-10. The replication of the virus is inhibited in human cells by intervening in the structure of these proteins. The present invention is also related to the use of agents, which comprise peptides and/or interfering RNA and/or lipidic compounds, said agents producing a negative modulation or alteration of the cytoskeleton to prevent or to treat the HIV infection. The invention provides means and methods for altering the cytoskeleton/filament structure of cells, as a result of which the infection of human cells by HIV is disturbed and can even be completely inhibited. The cytoskeleton is altered by reducing the amount of vimentin and/or keratin (e.g. by transcriptional control using interfering RNA) or by using peptides that disrupt the cytoskeleton.

摘要翻译： 本发明描述了通过负面调节或改变细胞骨架，更精确地形成中间细胞骨架细丝的蛋白质来抑制人免疫缺陷病毒（HIV）复制的方法，其中所述蛋白质是波形蛋白和/或角蛋白-10。 通过介入这些蛋白质的结构，人类细胞中病毒的复制被抑制。 本发明还涉及包含肽和/或干扰RNA和/或脂质化合物的试剂的用途，所述试剂产生细胞骨架的负调节或改变以预防或治疗HIV感染。 本发明提供用于改变细胞的细胞骨架/细丝结构的方法和方法，其结果是HIV的人细胞感染被干扰并且甚至可以被完全抑制。 通过减少波形蛋白和/或角蛋白的量（例如通过使用干扰RNA的转录控制）或通过使用破坏细胞骨架的肽来改变细胞骨架。

公开(公告)号：US09205128B2

公开(公告)日：2015-12-08

申请号：US13637845

申请日：2011-04-01

申请人： Celia Berta Fernandez Ortega ,  Anna Caridys Ramírez Suárez ,  Dionne Casillas Casanova ,  Taimi Emelia Paneque Guerrero ,  Raimundo Ubieta Gómez ,  Marta Dubed Echevarria ,  Leonor Margarita Navea Leyva ,  Lila Rosa Castellanos Serra ,  Carlos Antonio Duarte Cano ,  Viviana Falcón Cama ,  Osvaldo Reyes Acosta

发明人： Celia Berta Fernandez Ortega ,  Anna Caridys Ramírez Suárez ,  Dionne Casillas Casanova ,  Taimi Emelia Paneque Guerrero ,  Raimundo Ubieta Gómez ,  Marta Dubed Echevarria ,  Leonor Margarita Navea Leyva ,  Lila Rosa Castellanos Serra ,  Carlos Antonio Duarte Cano ,  Viviana Falcón Cama ,  Osvaldo Reyes Acosta

IPC分类号： A61K38/10 ,  C12N5/0783 ,  A61K31/713 ,  A61K31/19 ,  A61K38/17 ,  A61K45/06 ,  A61K31/7105 ,  A61K31/5575 ,  C07K14/47 ,  C12N15/113

CPC分类号： A61K38/10 ,  A61K31/19 ,  A61K31/5575 ,  A61K31/7105 ,  A61K31/713 ,  A61K38/1709 ,  A61K38/1748 ,  A61K45/06 ,  C07K14/47 ,  C07K14/4741 ,  C12N5/0636 ,  C12N15/113 ,  C12N15/1131 ,  C12N15/1132 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2320/31

摘要： The present invention describes a method to inhibit replication of the human immunodeficiency virus (HIV) by negatively modulating or altering the cytoskeleton, more precisely the proteins forming the intermediate cytoskeletal filaments, wherein the said proteins are vimentin and/or keratin-10. The replication of the virus is inhibited in human cells by intervening in the structure of these proteins. The present invention is also related to the use of agents, which comprise peptides and/or interfering RNA and/or lipidic compounds, said agents producing a negative modulation or alteration of the cytoskeleton to prevent or to treat the HIV infection. The invention provides means and methods for altering the cytoskeleton/filament structure of cells, as a result of which the infection of human cells by HIV is disturbed and can even be completely inhibited. The cytoskeleton is altered by reducing the amount of vimentin and/or keratin (e.g. by transcriptional control using interfering RNA) or by using peptides that disrupt the cytoskeleton.

摘要翻译： 本发明描述了通过负面调节或改变细胞骨架，更精确地形成中间细胞骨架细丝的蛋白质来抑制人免疫缺陷病毒（HIV）复制的方法，其中所述蛋白质是波形蛋白和/或角蛋白-10。 通过介入这些蛋白质的结构，人类细胞中病毒的复制被抑制。 本发明还涉及包含肽和/或干扰RNA和/或脂质化合物的试剂的用途，所述试剂产生细胞骨架的负调节或改变以预防或治疗HIV感染。 本发明提供用于改变细胞的细胞骨架/细丝结构的方法和方法，其结果是HIV的人细胞感染被干扰并且甚至可以被完全抑制。 通过减少波形蛋白和/或角蛋白的量（例如通过使用干扰RNA的转录控制）或通过使用破坏细胞骨架的肽来改变细胞骨架。