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1.发明授权Method and apparatus for the delivery of polynucleotide vaccines to mammalian skin 有权用于将哺乳动物皮肤递送多核苷酸疫苗的方法和装置公开(公告)号：US08979818B2
公开(公告)日：2015-03-17
申请号：US13065135
申请日：2011-03-15
CPC分类号： A61N1/327 , A61K39/12 , A61K2039/53 , A61K2039/54 , A61K2039/70 , A61N1/306 , C07K14/005 , C12N2770/24122 , C12N2770/24134
摘要： An object of the invention is to provide a method and apparatus for the delivery of polynucleotide vaccines into mammalian skin cells to increase T cell response and to reduce pain and discomfort due to long electric waveform application and due to muscle contractions. The method for the delivery of polynucleotide vaccines into mammalian skin cells includes the steps of: (a.) administering a polynucleotide vaccine into the skin at an administration site, (b.) applying a needle electrode to the skin in the vicinity to the administration site, and (c.) applying a sequence of at least three single, operator-controlled, independently programmed, narrow interval electrical waveforms, which have pulse intervals that are less than 100 milliseconds, to deliver the polynucleotide vaccine into the skin cells by electroporation. The sequence of at least three waveforms has one, two, or three of the following characteristics (1) at least two of the at least three waveforms differ from each other in waveform amplitude, (2) at least two of the at least three waveforms differ from each other in waveform width, and (3) a first waveform interval for a first set of two of the at least three waveforms is different from a second waveform interval for a second set of two of the at least three waveforms.
摘要翻译： 本发明的目的是提供一种用于将多核苷酸疫苗递送到哺乳动物皮肤细胞中以增加T细胞应答并减少由于长电波形应用引起的疼痛和不适以及由于肌肉收缩引起的疼痛和不适的方法和装置。 用于将多核苷酸疫苗递送到哺乳动物皮肤细胞中的方法包括以下步骤：（a）在给药部位将多核苷酸疫苗施用于皮肤，（b）将针电极施用于给药的附近的皮肤 站点，以及（c）应用脉冲间隔小于100毫秒的至少三个单个，操作者控制的，独立编程的窄间隔电波形序列，以通过电穿孔将多核苷酸疫苗递送到皮肤细胞中 。 至少三个波形的序列具有以下特征（1）中的一个，两个或三个，所述至少三个波形中的至少两个波形在波形幅度上彼此不同，（2）至少三个波形中的至少两个波形 在波形宽度上彼此不同，以及（3）至少三个波形中的两个的第一组的第一波形间隔与用于至少三个波形中的两个的第二组的第二波形间隔不同。
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2.发明申请METHOD AND APPARATUS FOR THE DELIVERY OF PLYNUCLEOTIDE VACCINES TO MAMMALIA SKIN 审中-公开将灭草鼠疫苗递送给妈妈皮肤的方法和装置公开(公告)号：US20110282265A1
公开(公告)日：2011-11-17
申请号：US12451536
申请日：2008-05-20
IPC分类号： A61M37/00
CPC分类号： A61N1/327 , A61K39/12 , A61K2039/53 , A61K2039/54 , A61K2039/70 , A61N1/306 , C07K14/005 , C12N2770/24122 , C12N2770/24134
摘要： An object of the invention is to provide a method and apparatus for the delivery of polynucleotide vaccines into mammalian skin cells to increase T cell response and reduce pain and discomfort due to long electric waveform application and due to muscle contractions. The method includes administering a polynucleotide vaccine into the skin at an administration site, (b) applying a needle electrode to the skin in the vicinity to the administration site, and (c) applying a sequence of at least three single, operator-controlled, independently programmed, narrow interval electrical waveforms, which have pulse intervals that are less than 100 milliseconds, to deliver the 15 polynucleotide vaccine into the skin cells by electroporation.
摘要翻译： 本发明的目的是提供一种用于将多核苷酸疫苗递送到哺乳动物皮肤细胞中以增加T细胞应答并减少由于长电波形应用引起的疼痛和不适以及由于肌肉收缩而引起的不适的方法和装置。 该方法包括在给药部位向皮肤施用多核苷酸疫苗，（b）将针电极施用于给药部位附近的皮肤，以及（c）施用至少三个单独的，操作者控制的， 具有脉冲间隔小于100毫秒的独立编程的窄间隔电波形，以通过电穿孔将15个多核苷酸疫苗递送到皮肤细胞中。
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3.发明申请METHOD AND APPARATUS FOR THE DELIVERY OF POLYNUCLEOTIDE CANCER VACCINES TO MAMMALIAN SKIN 审中-公开将多核苷酸疫苗递送给妈妈皮肤的方法和装置公开(公告)号：US20130123744A1
公开(公告)日：2013-05-16
申请号：US13138722
申请日：2010-03-26
申请人： Derin C. Walters , Alan D. King , Anna-Karin Maltais (formerly Anna-Karin Roos) , Britta Wahren , Kristian Haller (formerly Kristian Hallermalm) , Andreas Brave , Robert J. Walters
发明人： Richard E. Walters , Derin C. Walters , Alan D. King , Anna-Karin Maltais (formerly Anna-Karin Roos) , Britta Wahren , Kristian Haller (formerly Kristian Hallermalm) , Andreas Brave
IPC分类号： A61N1/30
CPC分类号： A61N1/306 , A61K39/00 , A61K39/0011 , A61K2039/53 , A61K2039/54 , A61N1/327 , C12N2770/24134
摘要： The invention provides a method and apparatus for the delivery of polynucleotide cancer vaccines to increase T cell response and reduce pain due to long electric waveform application and muscle contractions. The method includes the steps of: (a.) administering a polynucleotide cancer vaccine into the skin at an administration site, (b.) applying a needle electrode to the skin in the vicinity of the administration site, and (c.) applying a sequence of at least three electrical waveforms to deliver the polynucleotide cancer vaccine into skin cells by electroporation. The sequence has at least one of the following characteristics {1} at least two of the waveforms differ in amplitude, {2} at least two of the waveforms differ in width, and (3) a first waveform interval for a first set of two of the waveforms is different from a second waveform interval for a second set of two of the waveforms.
摘要翻译： 本发明提供了用于递送多核苷酸癌症疫苗以增加T细胞应答并减少由于长的电波形应用和肌肉收缩引起的疼痛的方法和装置。 该方法包括以下步骤：（a）在给药部位向皮肤施用多核苷酸癌症疫苗，（b）在给药部位附近向皮肤施用针电极，（c） 至少三个电波形的序列，以通过电穿孔将多核苷酸癌症疫苗递送到皮肤细胞中。 该序列具有以下特征中的至少一个{1}，至少两个波形幅度不同，{2}至少两个波形的宽度不同，（3）第一组二分之一的第一波形间隔 的波形与第二组波形的第二波形间隔不同。
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4.发明申请Method and apparatus for the delivery of polynucleotide vaccines to mammalian skin 审中-公开用于将哺乳动物皮肤递送多核苷酸疫苗的方法和装置公开(公告)号：US20110166501A1
公开(公告)日：2011-07-07
申请号：US13065135
申请日：2011-03-15
IPC分类号： A61M37/00
CPC分类号： A61N1/327 , A61K39/12 , A61K2039/53 , A61K2039/54 , A61K2039/70 , A61N1/306 , C07K14/005 , C12N2770/24122 , C12N2770/24134
摘要： An object of the invention is to provide a method and apparatus for the delivery of polynucleotide vaccines into mammalian skin cells to increase T cell response and to reduce pain and discomfort due to long electric waveform application and due to muscle contractions. The method for the delivery of polynucleotide vaccines into mammalian skin cells includes the steps of: (a.) administering a polynucleotide vaccine into the skin at an administration site, (b.) applying a needle electrode to the skin in the vicinity to the administration site, and (c.) applying a sequence of at least three single, operator-controlled, independently programmed, narrow interval electrical waveforms, which have pulse intervals that are less than 100 milliseconds, to deliver the polynucleotide vaccine into the skin cells by electroporation. The sequence of at least three waveforms has one, two, or three of the following characteristics (1) at least two of the at least three waveforms differ from each other in waveform amplitude, (2) at least two of the at least three waveforms differ from each other in waveform width, and (3) a first waveform interval for a first set of two of the at least three waveforms is different from a second waveform interval for a second set of two of the at least three waveforms.
摘要翻译： 本发明的目的是提供一种用于将多核苷酸疫苗递送到哺乳动物皮肤细胞中以增加T细胞应答并减少由于长电波形应用引起的疼痛和不适以及由于肌肉收缩引起的疼痛和不适的方法和装置。 用于将多核苷酸疫苗递送到哺乳动物皮肤细胞中的方法包括以下步骤：（a）在给药部位将多核苷酸疫苗施用于皮肤，（b）将针电极施用于给药的附近的皮肤 站点，以及（c）应用脉冲间隔小于100毫秒的至少三个单个，操作者控制的，独立编程的窄间隔电波形序列，以通过电穿孔将多核苷酸疫苗递送到皮肤细胞中 。 至少三个波形的序列具有以下特征（1）中的一个，两个或三个，所述至少三个波形中的至少两个波形在波形幅度上彼此不同，（2）至少三个波形中的至少两个波形 在波形宽度上彼此不同，以及（3）至少三个波形中的两个的第一组的第一波形间隔与用于至少三个波形中的两个的第二组的第二波形间隔不同。
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