公开(公告)号：US5955077A

公开(公告)日：1999-09-21

申请号：US465640

申请日：1995-06-05

申请人： Peter Andersen ,  .ANG.se Bengaard Andersen ,  Kaare Haslov ,  Anne Lund Sorensen

发明人： Peter Andersen ,  .ANG.se Bengaard Andersen ,  Kaare Haslov ,  Anne Lund Sorensen

IPC分类号： A61K39/04 ,  A61P31/06 ,  C07K14/35 ,  A61K39/00 ,  C07K1/00 ,  C12P21/06

CPC分类号： A61K39/04 ,  C07K14/35 ,  C12R1/19 ,  A61K2039/523 ,  A61K2039/53

摘要： The invention relates to novel secreted antigens from mycobacteria capable of evoking early (within 4 days) immunological responses from T-helper cells in the form of gamma-interferon release in memory immune animals after rechallenge infection with mycobacteria of the tuberculosis complex. The antigens are present in short term filtrates (ST-CF) from cultured mycobacteria belonging to the tuberculosis complex. One of these antigens, a polypeptide with an apparent molecular weight of 6 kDa, has been identified, and the DNA encoding the polypeptide has been cloned and sequenced. The antigens of the invention are believed useful especially in vaccines, but also in diagnostic compositions, especially for diagnosing infection with virulent mycobacteria. Also disclosed are nucleic acid fragments encoding the antigens as well as methods of immunizing animals/humans and methods of diagnosing tuberculosis.

摘要翻译： 本发明涉及能够以结核分枝杆菌复合体分枝杆菌感染后的记忆免疫动物中早期（4天内）诱发来自T-辅助细胞的免疫反应以γ-干扰素释放的形式的分枝杆菌的新型分泌型抗原。 抗原存在于来自属于结核病复合体的培养的分枝杆菌的短期滤液（ST-CF）中。 已经鉴定了这些抗原中的一种，表观分子量为6kDa的多肽，并且已经克隆并测序了编码该多肽的DNA。 认为本发明的抗原特别用于疫苗，而且在诊断组合物中尤其是用于诊断有毒分枝杆菌的感染是有用的。 还公开了编码抗原的核酸片段以及免疫动物/人的方法和诊断结核病的方法。