公开(公告)号：US06638769B2

公开(公告)日：2003-10-28

申请号：US09972918

申请日：2001-10-10

申请人： Jan Lilja ,  Sven-Erik Nilsson ,  Johnny Svensson ,  Annika Eriksson

发明人： Jan Lilja ,  Sven-Erik Nilsson ,  Johnny Svensson ,  Annika Eriksson

IPC分类号： G01N3372

CPC分类号： G01N21/272 ,  G01N21/03 ,  G01N21/0303 ,  G01N21/31 ,  G01N33/5094 ,  G01N33/72 ,  G01N2021/0321 ,  G01N2021/0325 ,  G01N2021/0346 ,  G01N2021/0389

摘要： A method for hemoglobin determination is described which includes the steps of introducing a sample of undiluted whole blood by capillary action into a disposable microcuvette having at least one cavity for receiving the sample. The cavity includes a dry essentially non-hygroscopic hemolysing agent, which is dissolved by the blood, hemolyses the red blood cells and releases the haemoglobin contained in the blood cells. A first absorption measurement at a wavelength range 490-520 nm is then performed directly on the sample in the microcuvette, and a second absorption measurement is performed to compensate for background interference.

摘要翻译： 描述了一种用于血红蛋白测定的方法，其包括以下步骤：通过毛细管作用将未稀释的全血样品引入到具有用于接收样品的至少一个空腔的一次性微型小瓶中。 空腔包括干燥的基本上不吸湿的溶血剂，其被血液溶解，溶血红细胞并释放血细胞中所含的血红蛋白。 然后直接在微量检测器中的样品上进行490-520nm波长范围的第一次吸收测量，并进行第二次吸收测量以补偿背景干扰。