公开(公告)号：US06476293B1

公开(公告)日：2002-11-05

申请号：US09410023

申请日：1999-10-01

申请人： Arthur G. Hunt ,  Glenn B. Collins ,  Christopher Lawrence ,  Qingshun Li ,  Santanu Dasgupta

发明人： Arthur G. Hunt ,  Glenn B. Collins ,  Christopher Lawrence ,  Qingshun Li ,  Santanu Dasgupta

IPC分类号： A01H300

CPC分类号： C12N9/1217 ,  C12N15/8281

摘要： An isolated gene fragment that encodes for acetate kinase, which confers disease resistance in plants is disclosed. The gene can be cloned into an expression vector to produce a recombinant DNA expression system suitable for insertion into cells to form a transgenic plant transformed with the gene fragment. A method for conferring disease resistance in plants that consists of growing plant host cells transformed with the expression system and expressing the gene conferring disease resistance to impart such resistance to host cells is also disclosed.

摘要翻译： 公开了编码乙酸激酶的分离的基因片段，其赋予植物抗病性。 该基因可克隆到表达载体中以产生适于插入细胞的重组DNA表达系统，以形成用该基因片段转化的转基因植物。 还公开了一种在植物中赋予抗病性的方法，其包括用表达系统转化的生长的植物宿主细胞并表达赋予疾病抗性以赋予宿主细胞这种抗性的基因。

公开(公告)号：US6018106A

公开(公告)日：2000-01-25

申请号：US116879

申请日：1998-07-16

申请人： Arthur G. Hunt ,  Qing-Shun Li ,  Jianjun Yang ,  Carol Von Lanken

发明人： Arthur G. Hunt ,  Qing-Shun Li ,  Jianjun Yang ,  Carol Von Lanken

IPC分类号： C07K14/39 ,  C07K14/395 ,  C12N15/82 ,  A01H5/00 ,  C12N5/14

CPC分类号： C12N15/8283 ,  C07K14/39 ,  C07K14/395 ,  C12N15/8261 ,  C12N15/8281 ,  C12N15/8282

摘要： Plants that accumulate the yeast polyadenylate binding protein (yPAB) display a range of abnormalities, including a characteristic chlorosis in leaves to a necrosis and pronounced inhibition of growth. The severity of these abnormalities reflects the levels of yeast PAB expression in the transgenic plants. In contrast, no obvious differences are seen in undifferentiated callus cultures that express the same range of yeast PAB. The expression of the yeast PAB1 gene in plants does not affect expression of the plant PAB gene family or alter poly(A) length in the total RNA population. It is proposed that the yeast PAB1 gene or its product interferes with as yet unidentified functions of PABs, which functions are manifest only in differentiated, developed plants. Surprisingly, transgenic plants expressing the yeast PAB1 gene are also observed to have a systemic acquired resistance (SAR) to bacterial, fungal and viral pathogens.

摘要翻译： 积累酵母多腺苷酸结合蛋白（yPAB）的植物显示一系列异常，包括叶片中的特征性萎黄症，坏死和显着抑制生长。 这些异常的严重程度反映了转基因植物中酵母PAB表达的水平。 相比之下，在表达相同范围的酵母PAB的未分化愈伤组织培养物中没有观察到明显的差异。 酵母PAB1基因在植物中的表达不影响植物PAB基因家族的表达，也不影响总RNA群体的poly（A）长度。 提出酵母PAB1基因或其产物干扰PAB的尚未识别的功能，其功能仅在差异化的开发植物中显现。 令人惊讶的是，也观察到表达酵母PAB1基因的转基因植物对细菌，真菌和病毒病原体具有系统性获得性抗性（SAR）。

公开(公告)号：US06342654B1

公开(公告)日：2002-01-29

申请号：US09444412

申请日：1999-11-22

申请人： Qingshun Li ,  Songhai Shen ,  Arthur G. Hunt ,  Sheng Yang He

发明人： Qingshun Li ,  Songhai Shen ,  Arthur G. Hunt ,  Sheng Yang He

IPC分类号： C12N509

CPC分类号： C12N15/8282 ,  C07K14/21 ,  C12N15/8281 ,  C12N15/8283

摘要： The use of an avr gene hrmA to induce systematic acquired resistance in plant cells, plant seeds, plant tissues and plants is disclosed. Also disclosed is the use of low level expression of promoters in combination with the hrmA gene to provide broad-spectrum pathogen resistance in plant cells, plant seeds, plant tissues and plants.

摘要翻译： 公开了使用avr基因hrmA在植物细胞，植物种子，植物组织和植物中诱导系统性获得性抗性。 还公开了与hrmA基因组合使用低水平表达启动子以在植物细胞，植物种子，植物组织和植物中提供广谱病原体抗性。