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公开(公告)号：US10899678B2
公开(公告)日：2021-01-26
申请号：US16633186
申请日：2019-05-16
申请人： Yuriy Borisovich Tolkachnikov , Konstantin Yur'evich Tolkachnikov , Yuriy Vladimirovich Kos'yanenko , Vyacheslav Anatol'yevich Titov , Dmitriy Nikolayevich Kuznetsov , Artur Mikhaylovich Gorbunov
发明人： Yuriy Borisovich Tolkachnikov , Konstantin Yur'evich Tolkachnikov , Yuriy Vladimirovich Kos'yanenko , Vyacheslav Anatol'yevich Titov , Dmitriy Nikolayevich Kuznetsov , Artur Mikhaylovich Gorbunov
摘要： The invention relates to agriculture, in particular to the processing of carbon-containing material to obtain a humus-containing product. A method for processing a carbon-containing material to obtain a humus-containing product by exposing it to a mixture of bacteria consisting of the strains Acinetobacter calcoaceticus VKPM (Russian National Collection of Industrial Microorganisms) V-4883, Pseudomonas denitrificans VKPM V-4884, Pseudomonas sp. “longa” VKPM V-4885 in an amount of (6-8)×109, (3-4)×109 and (2-3)×109 cells per 1 L of solution, respectively, in the presence of a phosphorus-containing additive, under aerobic conditions and while stirring, wherein the carbon-containing material is firstly ground to 0.5 mm in a wet mill while sapropel is simultaneously added in an amount of 5-10 wt %, and grinding is carried out for 30 minutes, the resulting mixture is sent into a mixer, into which aluminium industry waste in the form of dust from the cleaning of carbon electrodes with a particle size up to 0.5 mm in an amount of 10-20 wt % and a phosphorus-containing additive in an amount of 0.2-0.6 wt % are fed, mixing is carried out for 5-10 min, the resulting suspension is sent to a fermenter into which an inoculate has been fed beforehand in the form of a mixture of bacteria in an amount of 200-233 L per 1 m3 of suspension, and additionally stirring is performed while air is fed from 0.5 to 2.0 m3/min at a temperature of 15-20° C. for 28-30 h. The invention makes it possible to improve the quality of the target product.
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公开(公告)号：US11141764B2
公开(公告)日：2021-10-12
申请号：US16637283
申请日：2019-05-16
申请人： Yuriy Borisovich Tolkachnikov , Konstantin Yur'yevich Tolkachnikov , Yuriy Vladimirovich Kos'yanenko , Vyacheslav Anatol'yevich Titov , Dmitriy Nikolayevich Kuznetsov , Artur Mikhaylovich Gorbunov
发明人： Yuriy Borisovich Tolkachnikov , Konstantin Yur'yevich Tolkachnikov , Yuriy Vladimirovich Kos'yanenko , Vyacheslav Anatol'yevich Titov , Dmitriy Nikolayevich Kuznetsov , Artur Mikhaylovich Gorbunov
摘要： The invention relates to agriculture and can be used to restore soil fertility. A method for remediation of contaminated lands involves pouring and introducing a bioreagent to a depth into the soil prepared for purification. Remediation is carried out in 2 steps: in the first step, slit-like or round holes are made in the infected area to a depth of 25 cm, poured with water, afterwards, 5-6 hours later, this area is poured with a bioreagent in the form of a humus-containing suspension in an amount of up to 400 g per kg of soil containing strains: Acinetobacter calcoaceticus VKPM V-4883, Pseudomonas denitrificans VKPM-4884, Pseudomonas sp. “longa” VKPM V-4885, Rhodococcus erythropolis in an amount of (6-8)·109, (3-4)·109 (2-3)·109, (1-2)·109 cells per 1 L of solution, respectively, in the ratio, wt. % 4.8:2.4:1.7:1.0. In the second step, 8-10 days later, half the initial dose of the concentrate of the humus-containing suspension is introduced. After introduction of the second reduced dose of the humus-containing suspension, watering is continued for 14 days. The distance between the slit-like or round holes is 8-10 cm. The proposed method for remediation of contaminated lands provides for treatment efficiency and a reduction in the degree of decontamination from a high degree of contamination to the MPC (maximum permissible concentration) level, preservation of soil biocenosis and restoration of soil fertility.
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公开(公告)号：US20200246849A1
公开(公告)日：2020-08-06
申请号：US16637283
申请日：2019-05-16
申请人： Yuriy Borisovich TOLKACHNIKOV , Konstantin Yur'yevich TOLKACHNIKOV , Yuriy Vladimirovich KOS'YANENKO , Vyacheslav Anatol'yevich TITOV , Dmitriy Nikolayevich KUZNETSOV , Artur Mikhaylovich GORBUNOV
发明人： Yuriy Borisovich TOLKACHNIKOV , Konstantin Yur'yevich TOLKACHNIKOV , Yuriy Vladimirovich KOS'YANENKO , Vyacheslav Anatol'yevich TITOV , Dmitriy Nikolayevich KUZNETSOV , Artur Mikhaylovich GORBUNOV
摘要： The invention relates to agriculture and can be used to restore soil fertility. A method for remediation of contaminated lands involves pouring and introducing a bioreagent to a depth into the soil prepared for purification. Remediation is carried out in 2 steps: in the first step, slit-like or round holes are made in the infected area to a depth of 25 cm, poured with water, afterwards, 5-6 hours later, this area is poured with a bioreagent in the form of a humus-containing suspension in an amount of up to 400 g per kg of soil containing strains: Acinetobacter calcoaceticus VKPM-4883, Pseudomonas denitrificans VKPM-4884, Pseudomonas sp. “longa”. VKPM-4885, Rhodococcus erythropolis in an amount of (6-8)·109, (3-4)·109 (2-3)·109, (1-2)·109 cells per 1 L of solution, respectively, in the ration, wt % 4.8:2.4:1.7:1.0. In the second step, 8-10 days later, half the initial dose of the concentrate of the humus-containing suspension is introduced. After introduction of the second reduced dose of the humus-containing suspension, watering is continued for 14 days. The distance between the slit-like or round holes is 8-10 cm. The proposed method for remediation of contaminated lands provides for treatment efficiency and a reduction in the degree of decontamination from a high degree of contamination to the MPC (maximum permissible concentration) level, preservation of soil biocenosis and restoration of soil fertility.
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