公开(公告)号：US09134247B2

公开(公告)日：2015-09-15

申请号：US13374225

申请日：2011-12-16

Applicant: Atanu Sengupta ,  Frank E. Inscore ,  Chetan Shende ,  Michael J. Donahue ,  Stuart Farquharson

Inventor： Atanu Sengupta ,  Frank E. Inscore ,  Chetan Shende ,  Michael J. Donahue ,  Stuart Farquharson

IPC: G01N21/65 ,  G01N33/58

CPC classification number: G01N21/658 ,  G01N33/54373 ,  G01N33/58 ,  G01N33/587

Abstract: A SERS method and apparatus employ a sample device having support structure including a first material containing a SER-active metal functionalized with a binding agent having specific capability for binding a designated target analyte. An analyte sample is introduced upon the functionalized SER-active metal; conditions to effect binding of the target analyte to the binding agent are maintained; unbound chemicals, biochemicals, or biologicals are removed; a second SER-active material is introduced to cause it to attach to the bound target analyte; the support structure is irradiated to generate a SER spectrum, with the first and second SER-active materials acting in concert; and the SER spectrum is detected and analyzed to determine the presence and quantity of the target analyte. Alternatively, the second SER-active material may be functionalized with a binding agent, with the procedure being modified accordingly.

Abstract translation: SERS方法和装置使用具有支撑结构的样品装置，该样品装置包括含有用具有特异性结合指定靶标分析物能力的结合剂官能化的SER-活性金属的第一材料。 将分析物样品引入功能化的SER-活性金属上; 维持目标分析物与结合剂结合的条件; 未结合的化学物质，生物化学物质或生物制品被去除; 引入第二SER活性材料以使其附着于结合的靶分析物; 照射支撑结构以产生SER光谱，其中第一和第二SER活性材料一致; 并检测和分析SER光谱，以确定目标分析物的存在和数量。 或者，第二SER活性材料可以用粘合剂进行官能化，其过程被相应地改变。

公开(公告)号：US20130157254A1

公开(公告)日：2013-06-20

申请号：US13374225

申请日：2011-12-16

Applicant: Atanu SENGUPTA ,  Frank E. INSCORE ,  Chetan SHENDE ,  Michael J. DONAHUE ,  Stuart FARQUHARSON

Inventor： Atanu SENGUPTA ,  Frank E. INSCORE ,  Chetan SHENDE ,  Michael J. DONAHUE ,  Stuart FARQUHARSON

IPC: G01N21/65 ,  C12M1/40 ,  C12M1/34 ,  C12Q1/70

CPC classification number: G01N21/658 ,  G01N33/54373 ,  G01N33/58 ,  G01N33/587

Abstract: A SERS method and apparatus employ a sample device having support structure including a first material containing a SER-active metal functionalized with a binding agent having specific capability for binding a designated target analyte. An analyte sample is introduced upon the functionalized SER-active metal; conditions to effect binding of the target analyte to the binding agent are maintained; unbound chemicals, biochemicals, or biologicals are removed; a second SER-active material is introduced to cause it to attach to the bound target analyte; the support structure is irradiated to generate a SER spectrum, with the first and second SER-active materials acting in concert; and the SER spectrum is detected and analyzed to determine the presence and quantity of the target analyte. Alternatively, the second SER-active material may be functionalized with a binding agent, with the procedure being modified accordingly.