公开(公告)号：US10000744B2

公开(公告)日：2018-06-19

申请号：US15023579

申请日：2014-09-23

Applicant: BRASKEM S.A. ,  Avram Michael Slovic

Inventor： Avram Michael Slovic ,  Iuri Estrada Gouvea ,  Daniel Johannes Koch ,  Felipe Galzerani

IPC: C12N1/19 ,  C12N1/21 ,  C12N9/16 ,  C12N9/10 ,  C12P7/04

CPC classification number: C12N9/16 ,  C12N9/13 ,  C12P7/04 ,  C12Y301/02011

Abstract: The disclosure provides engineered enzymes that are capable of mediating the conversion of acetoacetyl-CoA to acetoacetate that do not react with the same order of magnitude with acetyl-CoA as they do with acetoacetyl-CoA (e.g., the engineered enzymes have a specific acetoacetyl-CoA hydrolase activity at least 10× higher than its acetyl-CoA hydrolase activity). Additionally, the disclosure provides modified microorganisms that comprise the engineered enzymes disclosed herein and methods of using same.

公开(公告)号：US20160208228A1

公开(公告)日：2016-07-21

申请号：US15023579

申请日：2014-09-23

Applicant: Avram Michael SLOVIC ,  BRASKEM S.A.

Inventor： Avram Michael Slovic ,  Iuri Estrada Gouvea ,  Daniel Johannes Koch ,  Felipe Galzerani

IPC: C12N9/16

CPC classification number: C12N9/16 ,  C12N9/13 ,  C12P7/04 ,  C12Y301/02011

Abstract: The disclosure provides engineered enzymes that are capable of mediating the conversion of acetoacetyl-CoA to acetoacetate that do not react with the same order of magnitude with acetyl-CoA as they do with acetoacetyl-CoA (e.g., the engineered enzymes have a specific acetoacetyl-CoA hydrolase activity at least 10x higher than its acetyl-CoA hydrolase activity). Additionally, the disclosure provides modified microorganisms that comprise the engineered enzymes disclosed herein and methods of using same.

Abstract translation: 本公开提供了能够介导乙酰乙酰辅酶A向乙酰乙酸酯转化的工程化酶，其与乙酰辅酶A不具有与乙酰辅酶A相同数量级的反应（例如，工程化酶具有特定的乙酰乙酰基 - CoA水解酶活性比其乙酰-CoA水解酶活性高至少10倍）。 另外，本公开提供包含本文公开的工程化酶的修饰的微生物及其使用方法。