公开(公告)号：US08470574B2

公开(公告)日：2013-06-25

申请号：US12989728

申请日：2009-05-07

申请人： Atsushi Toda ,  Sachio Iwai ,  Yoshiaki Nishiya ,  Takeshi Miyata ,  Aya Oosato ,  Shinya Kumagai ,  Toshihide Yamada

发明人： Atsushi Toda ,  Sachio Iwai ,  Yoshiaki Nishiya ,  Takeshi Miyata ,  Aya Oosato ,  Shinya Kumagai ,  Toshihide Yamada

IPC分类号： C12N9/14 ,  C07K1/00

CPC分类号： C12N9/80 ,  A23L33/175 ,  C12P13/04 ,  C12P13/06 ,  C12P41/007

摘要： The present invention provides an L-aminoacylase which is able to produce L-tert-leucine being useful as an intermediate for pharmaceuticals.A protein which is characterized in being represented by any of the following (a) to (d): (a) a protein coded by a gene consisting of a nucleic acid sequence shown in SEQ ID No: 1; (b) a protein consisting of an amino acid sequence shown in SEQ ID No: 2; (c) a protein coded by a polynucleotide which hybridizes under a stringent condition with a nucleic acid sequence which is complementary to the nucleic acid sequence shown in SEQ ID No: 1 and having an L-succinylaminoacylase activity; and (d) a protein which consists of an amino acid sequence where one or several amino acid (s) is/are substituted, deleted, inserted and/or added in the protein consisting of the amino acid sequence shown in SEQ ID No: 2 and has an L-succinylaminoacylase activity.

摘要翻译： 本发明提供能够产生L-叔亮氨酸的L-氨基酸酰基酶，其可用作药物的中间体。 特征在于以下（a）〜（d）中任一项所示的蛋白质：（a）由由SEQ ID No：1所示的核酸序列组成的基因编码的蛋白质; （b）由SEQ ID No：2所示的氨基酸序列组成的蛋白质; （c）由严格条件下与SEQ ID No：1所示核酸序列互补且具有L-琥珀酰氨基酰化酶活性的核酸序列杂交的多核苷酸编码的蛋白质; 和（d）由氨基酸序列组成的蛋白质，其中一个或几个氨基酸被取代，缺失，插入和/或添加在由SEQ ID No：2所示的氨基酸序列组成的蛋白质中 并具有L-琥珀酰氨基酰化酶活性。

公开(公告)号：US20110244530A1

公开(公告)日：2011-10-06

申请号：US12989728

申请日：2009-05-07

申请人： Atsushi Toda ,  Sachio Iwai ,  Yoshiaki Nishiya ,  Takeshi Miyata ,  Aya Oosato ,  Shinya Kumagai ,  Toshihide Yamada

发明人： Atsushi Toda ,  Sachio Iwai ,  Yoshiaki Nishiya ,  Takeshi Miyata ,  Aya Oosato ,  Shinya Kumagai ,  Toshihide Yamada

IPC分类号： C12P13/06 ,  C12P13/04 ,  C07H21/04 ,  C12N9/80

CPC分类号： C12N9/80 ,  A23L33/175 ,  C12P13/04 ,  C12P13/06 ,  C12P41/007

摘要： The present invention provides an L-aminoacylase which is able to produce L-tert-leucine being useful as an intermediate for pharmaceuticals.A protein which is characterized in being represented by any of the following (a) to (d): (a) a protein coded by a gene consisting of a nucleic acid sequence shown in SEQ ID No: 1; (b) a protein consisting of an amino acid sequence shown in SEQ ID No: 2; (c) a protein coded by a polynucleotide which hybridizes under a stringent condition with a nucleic acid sequence which is complementary to the nucleic acid sequence shown in SEQ ID No: 1 and having an L-succinylaminoacylase activity; and (d) a protein which consists of an amino acid sequence where one or several amino acid (s) is/are substituted, deleted, inserted and/or added in the protein consisting of the amino acid sequence shown in SEQ ID No: 2 and has an L-succinylaminoacylase activity.

摘要翻译： 本发明提供能够产生L-叔亮氨酸的L-氨基酸酰基酶，其可用作药物的中间体。 特征在于以下（a）〜（d）中任一项所示的蛋白质：（a）由由SEQ ID No：1所示的核酸序列组成的基因编码的蛋白质; （b）由SEQ ID No：2所示的氨基酸序列组成的蛋白质; （c）由严格条件下与SEQ ID No：1所示核酸序列互补且具有L-琥珀酰氨基酰化酶活性的核酸序列杂交的多核苷酸编码的蛋白质; 和（d）由氨基酸序列组成的蛋白质，其中一个或几个氨基酸被取代，缺失，插入和/或添加在由SEQ ID No：2所示的氨基酸序列组成的蛋白质中 并具有L-琥珀酰氨基酰化酶活性。