公开(公告)号：US07341847B2

公开(公告)日：2008-03-11

申请号：US10407009

申请日：2003-04-02

Applicant: Shu Wang ,  Beihui Liu ,  Xu Wang

Inventor： Shu Wang ,  Beihui Liu ,  Xu Wang

IPC: C12P12/00 ,  C07H21/04 ,  C12N15/00 ,  C12N5/00

CPC classification number: A61K48/0058 ,  C07H21/04 ,  C12N15/86 ,  C12N2750/14143

Abstract: The invention relates to a chimeric promoter construct useful for neuronal cell specific gene expression. The invention provides a recombinant nucleic acid molecule that comprises a neuronal cell specific promoter such as platelet-derived growth factor β-chain promoter, operably linked to a heterologous enhancer which enhances the transcriptional activity of the promoter, such as cytomegalovirus immediate early gene enhancer. The promoter construct according to the invention can increase and prolong gene expression in neuronal cells and may be advantageously used in gene therapy of neuronal disorders.

Abstract translation: 本发明涉及可用于神经细胞特异性基因表达的嵌合启动子构建体。 本发明提供了一种重组核酸分子，其包含神经元细胞特异性启动子，例如血小板衍生生长因子β-链启动子，可操作地连接到增强启动子如巨细胞病毒立即早期基因增强子的转录活性的异源增强子。 根据本发明的启动子构建体可以增加并延长神经元细胞中的基因表达，并可有利地用于神经元疾病的基因治疗。