公开(公告)号：US4593004A

公开(公告)日：1986-06-03

申请号：US526034

申请日：1983-08-24

申请人： Laszlo Boross ,  Ivan Daroczi ,  Katalin Ivony nee Kaldive ,  Gabor Seres ,  Bela Szanjani ,  Jozsef Szejtli

发明人： Laszlo Boross ,  Ivan Daroczi ,  Katalin Ivony nee Kaldive ,  Gabor Seres ,  Bela Szanjani ,  Jozsef Szejtli

IPC分类号： C12N11/08 ,  C12N11/10 ,  C12P19/18 ,  C12N11/06 ,  C12N11/02

CPC分类号： C12P19/18 ,  C12N11/08 ,  C12N11/10

摘要： The invention relates to a process for the preparation of a cyclodextrine glycosyltransferase enzyme.The immobilization can be carried out by two methods. According to the first method a cyclodextrine glycosyltransferase enzyme is activated with a solution of a carbodiimide compound and the thus treated enzyme is applied in a solution having a pH value of 4.5-8.5 onto a polysaccharide derivative comprising at least 0.1 m-equiv./g of free amino group. According to the second method the enzyme is applied in a solution having a pH value of 4.5-8.5 onto a polymer produced from an acrylic acid and/or methacrylic acid or acrylic amide and/or methacrylic amide monomer by means of a cross-linking agent of the acrylic or allylic type which polymer comprises at least 0.1 m-equiv./g of functional carboxy group, activating the polymer with a solution of a carbodiimide compound and washing and if desired drying the product thus obtained.The advantage of the process of the present invention is that the thus immobilized enzyme--contrary to the dissolved enzyme--is suitable for longlasting application and can be readily used on industrial scale continuous production.

摘要翻译： 本发明涉及一种制备环糊精糖基转移酶的方法。 固定化可以通过两种方法进行。 根据第一种方法，环糊精糖基转移酶用碳二亚胺化合物的溶液活化，将这样处理过的酶在pH值为4.5-8.5的溶液中施用到包含至少0.1m-准/克 的游离氨基。 根据第二种方法，将酶以pH值为4.5-8.5的溶液施用到由丙烯酸和/或甲基丙烯酸或丙烯酰胺和/或甲基丙烯酰胺单体通过交联剂产生的聚合物上 的丙烯酸或烯丙基类型，该聚合物包含至少0.1m比例的功能性羧基，用碳二亚胺化合物溶液活化聚合物，并且如果需要，干燥所得到的产物。 本发明方法的优点是与溶解的酶相反的固定化酶适用于长时间应用，并且可以容易地用于工业规模的连续生产。