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公开(公告)号：US20140011212A1
公开(公告)日：2014-01-09
申请号：US13982596
申请日：2012-01-30
申请人： Andreas Frei , Bernd Wollscheid , Ock-Youm Jeon , Erick Carreira
发明人： Andreas Frei , Bernd Wollscheid , Ock-Youm Jeon , Erick Carreira
IPC分类号： G01N33/566
CPC分类号： G01N33/566 , C07D495/04 , G01N33/582 , G01N2333/70596 , Y02P20/55
摘要： The present invention relates to trifunctional crosslinking reagents carrying (i) a ligand-reactive group for conjugation to a ligand of interest having at least one binding site on a target glycoprotein receptor, (ii) an optionally protected aromatic hydrazine group for the capturing of oxidized receptor-glycopeptides and (iii) an affinity group for the detection, isolation and purification of captured glycopeptides, their methods of production, as well as their use in methods for detecting, identifying and characterizing interactions between ligands and their corresponding glycoprotein target receptors on living cells and in biological fluids.
摘要翻译： 本发明涉及三官能交联剂，其携带（i）用于与靶糖配位体上至少有一个结合位点缀合的目的配体的配体 - 反应性基团，（ii）任选保护的芳族肼基团，用于捕获氧化的 受体 - 糖肽和（iii）用于检测，分离和纯化捕获的糖肽，其生产方法的亲和基团，以及它们用于检测，鉴定和表征配体与其相应的生物体内糖蛋白靶受体之间的相互作用的方法 细胞和生物液体。
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2.发明申请METHODS FOR CHARACTERIZING GLYCOPROTEINS AND GENERATING ANTIBODIES FOR SAME 有权表征GLYCOPROTEINS和产生抗体的方法公开(公告)号：US20110237775A1
公开(公告)日：2011-09-29
申请号：US13007384
申请日：2011-01-14
CPC分类号： C07K16/28
摘要： The invention provides methods for preparing a peptide useful in generating an antibody specific for the glycosylated form of a glycopolypeptide wherein the glycosylated portion is isolated and a peptide corresponding to amino acids adjacent to a glycosylated N-linked glycosylation site is prepared.
摘要翻译： 本发明提供了制备可用于产生糖基化形式的糖多肽特异性抗体的肽的方法，其中分离出糖基化部分，并且制备了与糖基化N-连接糖基化位点相邻的氨基酸相对应的肽。
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3.发明授权公开(公告)号：US08207113B2
公开(公告)日：2012-06-26
申请号：US13007384
申请日：2011-01-14
CPC分类号： C07K16/28
摘要： The invention provides methods for preparing a peptide useful in generating an antibody specific for the glycosylated form of a glycopolypeptide wherein the glycosylated portion is isolated and a peptide corresponding to amino acids adjacent to a glycosylated N-linked glycosylation site is prepared.
摘要翻译： 本发明提供了制备可用于产生糖基化形式的糖多肽特异性抗体的肽的方法，其中分离出糖基化部分，并且制备了与糖基化N-连接糖基化位点相邻的氨基酸相对应的肽。
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4.发明授权公开(公告)号：US08222208B2
公开(公告)日：2012-07-17
申请号：US13007393
申请日：2011-01-14
CPC分类号： C07K16/28
摘要： The invention provides methods for preparing a peptide as an immunogen to generate an antibody specific for the deglycosylated form of a glycopolypeptide by preparing a peptide that includes an N-linked glycosylation site of said glycopolypeptide and identifying a peptide sequence that includes said glycosylated site and preparing said peptide wherein N of said site is converted to D.
摘要翻译： 本发明提供了制备肽作为免疫原的方法，通过制备包含所述糖多肽的N-连接的糖基化位点的肽并鉴定包含所述糖基化位点的肽序列并制备了包含所述糖基化位点的肽序列，从而产生对糖多糖的去糖基化形式特异性的抗体 所述肽，其中所述位点的N转化为D.
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5.发明授权公开(公告)号：US07879799B2
公开(公告)日：2011-02-01
申请号：US11891487
申请日：2007-08-09
CPC分类号： C07K16/28
摘要： The invention provides methods for generating an antibody specific for the deglycosylated form of a glycopolypeptide using a peptide corresponding to an N-linked glycosylation site of a glycopolypeptide. The invention additionally provides methods for generating an antibody specific for a glycopolypeptide using a peptide corresponding to amino acids adjacent to an authentic N-linked glycosylation site.
摘要翻译： 本发明提供使用对应于糖多肽的N-连接糖基化位点的肽产生对糖多糖形式的去糖基化形式特异的抗体的方法。 本发明另外提供了使用对应于与真实N-连接的糖基化位点相邻的氨基酸的肽来产生对糖多肽特异性的抗体的方法。
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公开(公告)号：US09766235B2
公开(公告)日：2017-09-19
申请号：US13982596
申请日：2012-01-30
申请人： Andreas Frei , Bernd Wollscheid , Ock-Youm Jeon , Erick Carreira
发明人： Andreas Frei , Bernd Wollscheid , Ock-Youm Jeon , Erick Carreira
IPC分类号： G01N33/566 , C07D495/04 , G01N33/58
CPC分类号： G01N33/566 , C07D495/04 , G01N33/582 , G01N2333/70596 , Y02P20/55
摘要： The present invention relates to trifunctional crosslinking reagents carrying (i) a ligand-reactive group for conjugation to a ligand of interest having at least one binding site on a target glycoprotein receptor, (ii) an optionally protected aromatic hydrazine group for the capturing of oxidized receptor-glycopeptides and (iii) an affinity group for the detection, isolation and purification of captured glycopeptides, their methods of production, as well as their use in methods for detecting, identifying and characterizing interactions between ligands and their corresponding glycoprotein target receptors on living cells and in biological fluids.
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7.发明申请METHODS FOR CHARACTERIZING GLYCOPROTEINS AND GENERATING ANTIBODIES FOR SAME 有权表征GLYCOPROTEINS和产生抗体的方法公开(公告)号：US20110230640A1
公开(公告)日：2011-09-22
申请号：US13007393
申请日：2011-01-14
CPC分类号： C07K16/28
摘要： The invention provides methods for preparing a peptide as an immunogen to generate an antibody specific for the deglycosylated form of a glycopolypeptide by preparing a peptide that includes an N-linked glycosylation site of said glycopolypeptide and identifying a peptide sequence that includes said glycosylated site and preparing said peptide wherein N of said site is converted to D.
摘要翻译： 本发明提供了制备肽作为免疫原的方法，通过制备包含所述糖多肽的N-连接的糖基化位点的肽并鉴定包含所述糖基化位点的肽序列并制备了包含所述糖基化位点的肽序列，从而产生对糖多糖的去糖基化形式特异性的抗体 所述肽，其中所述位点的N转化为D.
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8.发明申请公开(公告)号：US20080108152A1
公开(公告)日：2008-05-08
申请号：US11891487
申请日：2007-08-09
申请人： Dan Martin , Bernd Wollscheid , Rudolf Aebersold , Julian Watts
发明人： Dan Martin , Bernd Wollscheid , Rudolf Aebersold , Julian Watts
IPC分类号： C07K16/18
CPC分类号： C07K16/28
摘要： The invention provides methods for generating an antibody specific for the deglycosylated form of a glycopolypeptide using a peptide corresponding to an N-linked glycosylation site of a glycopolypeptide. The invention additionally provides methods for generating an antibody specific for a glycopolypeptide using a peptide corresponding to amino acids adjacent to an authentic N-linked glycosylation site.
摘要翻译： 本发明提供使用对应于糖多肽的N-连接糖基化位点的肽产生对糖多糖形式的去糖基化形式特异的抗体的方法。 本发明另外提供了使用对应于与真实N-连接的糖基化位点相邻的氨基酸的肽来产生对糖多肽特异性的抗体的方法。
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