公开(公告)号：US5053328A

公开(公告)日：1991-10-01

申请号：US908777

申请日：1986-09-18

申请人： Bernhard Muetze ,  Christian Wandrey ,  Wolfgang Leuchtenberger ,  Toshihisa Ohshima

发明人： Bernhard Muetze ,  Christian Wandrey ,  Wolfgang Leuchtenberger ,  Toshihisa Ohshima

IPC分类号： C12N9/06 ,  C12P13/04 ,  C12P13/06 ,  C12R1/07

CPC分类号： C12P13/06 ,  C12P13/04 ,  Y10S435/832 ,  Y10S435/833 ,  Y10S435/834 ,  Y10S435/835 ,  Y10S435/836 ,  Y10S435/837 ,  Y10S435/838 ,  Y10S435/839

摘要： The preparation of an L-amino acid, particularly valine, leucine, isoleucine, alanine and phenylalanine, from the corresponding .alpha.-keto carboxylic acid by bacterial fermentation in the presence of ammonium ions is carried out with the aid of thermophilic Bacillus strains at temperatures above 45.degree. C., in particular above 60.degree. C. Bacillus strains DSM 406, 452, 461, 42, 463, 465 and 466 are particularly suitable for this purpose. The greater solubility of the amino acid at the elevated fermentation temperature permits the separation out of the amino acid from the reaction mixture simply by cooling, whereafter the depleted reaction mixture can be pumped back into the fermenter. Especially favorable yields are achieved by supplying oxygen to the fermenter to an amount of less than about 20% dissolved oxygen.

摘要翻译： 在铵离子存在下通过细菌发酵从相应的α-酮羧酸制备L-氨基酸，特别是缬氨酸，亮氨酸，异亮氨酸，丙氨酸和苯丙氨酸，借助于嗜热芽孢杆菌菌株，在温度高于 45℃，特别是高于60℃。芽孢杆菌菌株DSM 406,452,461,42,463,465和466特别适用于此目的。 氨基酸在升高的发酵温度下的更大的溶解度允许简单地通过冷却从反应混合物中分离出氨基酸，然后将贫化的反应混合物泵送回发酵罐。 通过向发酵罐供应氧气至小于约20％的溶解氧的量来实现特别有利的产率。