公开(公告)号：US20070231923A1

公开(公告)日：2007-10-04

申请号：US11397522

申请日：2006-04-03

申请人： Brendi Cumberland ,  Maha El-Sayed ,  Alan Fujii ,  Scott Manske ,  Elias Shaheen

发明人： Brendi Cumberland ,  Maha El-Sayed ,  Alan Fujii ,  Scott Manske ,  Elias Shaheen

IPC分类号： G01N33/558

CPC分类号： G01N33/558 ,  G01N31/22 ,  G01N2001/028 ,  Y10S435/97 ,  Y10S436/807 ,  Y10S977/70 ,  Y10S977/773 ,  Y10S977/788 ,  Y10S977/918

摘要： Provided is sampling and testing device for the detection of specific molds, allergens, viruses, bacteria, fungi, and other protein containing substances. Embodiments of the device include a sampling member slideably engaged with a base that contains a lateral flow strip adapted to detect specific analytes of interest. The sampling member defines a solvent reservoir that stores an elution solvent in a fluid-tight manner before the device is used to sample and test environmental surfaces. During slideable withdrawal of the sampling member from the base, the elution solvent stored in the reservoir is automatically released to a wick assembly of the sampling member. The wick assembly includes a wick adapted to receive, distribute, and retain the elution solvent. After a user samples an environmental surface for an analyte of interest with the elution solvent wetted wick, the sampling member is returned to the base where the wick contacts the lateral flow strip contained in the base. The wick transfers at least a portion of analyte and the elution solvent to the lateral flow strip for the calorimetric detection of specific allergens, viruses, bacteria, and other protein containing substances in the sample. The colorimetric results of the test are displayed through a window in the base.

摘要翻译： 提供了用于检测特定霉菌，过敏原，病毒，细菌，真菌和其他含蛋白质物质的取样和检测装置。 该装置的实施例包括可滑动地与基座接合的采样构件，其包含适于检测感兴趣的特定分析物的侧向流动条。 采样构件在使用该装置对环境表面进行采样和测试之前，限定了以流体密封的方式存储洗脱溶剂的溶剂储存器。 在将取样构件从基底滑动取出时，储存在储存器中的洗脱溶剂自动释放到取样构件的芯组件上。 芯组件包括适于接收，分配和保留洗脱溶剂的芯。 在用户使用洗脱溶剂润湿的吸管对用于所关注的分析物的环境表面进行采样之后，采样部件返回到基座，其中芯接触包含在基部中的侧向流动条。 芯芯将至少一部分分析物和洗脱溶剂转移到侧流条上，以对样品中的特定过敏原，病毒，细菌和其他含蛋白质的物质进行量热检测。 测试的比色结果通过基座中的窗口显示。

公开(公告)号：US20050287616A1

公开(公告)日：2005-12-29

申请号：US10880783

申请日：2004-06-29

申请人： Adam Gross ,  Brendi Cumberland ,  Kelly Dickson ,  Steven Bromberg

发明人： Adam Gross ,  Brendi Cumberland ,  Kelly Dickson ,  Steven Bromberg

IPC分类号： G01N33/53 ,  G01N33/537 ,  G01N33/542

CPC分类号： G01N33/5306

摘要： The present invention encompasses a method for performing an antibody-antigen assay using an ion-exchange technique prior to performing an antibody-antigen assay. Actives from protein-denaturing solutions are physically removed from the test sample, preferably by using an ion-exchange resin. The present invention also includes a method of performing an antibody-antigen assay, which does not use coating antibody on the sample holder. These methods for removing or eliminating interferences can be used in an ELISA to more effectively, efficiently and promptly screen formulations containing protein-denaturing compounds for efficacy against proteins in common allergens and detect and quantify such proteins.

摘要翻译： 本发明包括在进行抗体 - 抗原测定之前使用离子交换技术进行抗体 - 抗原测定的方法。 来自蛋白质变性溶液的活性物质优选通过使用离子交换树脂从测试样品中物理去除。 本发明还包括在样品架上不使用包被抗体的抗体 - 抗原测定方法。 用于去除或消除干扰物的这些方法可以用于ELISA中以更有效，高效和及时地筛选含有蛋白质变性化合物的制剂，以用于对普通变应原中的蛋白质的功效，并检测和定量这些蛋白质。