Methods and compositions for rapid in vitro propagation of Swertia chirata
    1.
    发明授权
    Methods and compositions for rapid in vitro propagation of Swertia chirata 有权
    Swertia chirata快速体外繁殖的方法和组成

    公开(公告)号:US07238527B2

    公开(公告)日:2007-07-03

    申请号:US10917919

    申请日:2004-08-13

    IPC分类号: C12N5/02

    CPC分类号: A01H4/005

    摘要: The present disclosure relates to methods and compositions for in vitro cultivation of species of Swertia, e.g. Swertia chirata (Ham.). The disclosure provides culture media comprising Murashige and Skoog (MS) basal culture medium, plant hormones preferably selected from the group consisting of benzyladenine (BAP), gibberellic acid (GA3), and auxins, and other additives, e.g. sucrose and agar. Preferably, auxins are selected from the group consisting of indole acetic acid (IAA), indole butyric acid (IBA), and naphthalene acetic acid (NAA). Individual plant hormone concentrations are preferably from about 0.5 mg/L to about 5.0 mg/L. The disclosure provides methods of in vitro cultivation of Swertia chirata comprising contacting preferably axillary bud and/or shoot apex explants with an initiation medium comprising a modified MS basal culture medium, BAP, IAA, IBA, and NAA to produce a primary explant, contacting the primary explant with a shoot propagation medium comprising, a modified MS basal culture medium, BAP, GA3, and IAA to produce a secondary explant, contacting a secondary explant with a rooting medium comprising a modified MS basal culture medium, IAA, IBA, and NAA. The methods and compositions of the invention are capable of inducing extraordinarily rapid in vitro propagation of Swertia chirata. The methods and compositions of the disclosure may be useful for conservation of this threatened species as well as producing bulk quantities, e.g. gram, kilogram or more, of plant material for medicinal

    摘要翻译: 本公开涉及用于体外培养Swertia物种的方法和组合物,例如 芝士(火鸡)。 本公开提供了包含Murashige和Skoog(MS)基础培养基的培养基,优选选自苄基腺嘌呤(BAP),赤霉酸(GA 3 N 3)和植物生长素的植物激素和其它添加剂 ,例如 蔗糖和琼脂。 优选地,生长素选自吲哚乙酸(IAA),吲哚丁酸(IBA)和萘乙酸(NAA)。 个体植物激素浓度优选为约0.5mg / L至约5.0mg / L。 本公开提供了Swertia chirata的体外培养方法,其包括优选使腋芽和/或茎顶端外植体与包含修饰的MS基础培养基BAP,IAA,IBA和NAA的起始培养基接触以产生初级外植体, 初级外植体,其具有包含修饰的MS基础培养基BAP,GA 3 N和IAA的芽繁殖培养基以产生第二外植体,使次生外植体与包含修饰的MS基础培养物的生根培养基接触 中等,IAA,IBA和NAA。 本发明的方法和组合物能够诱导Swertia chirata的异常快速的体外繁殖。 本公开的方法和组合物可用于保护该受威胁的物种以及产生大量的物质,例如, 克,千克以上的药用植物材料

    Methods and compositions for rapid in vitro propagation of Swertia chirata
    2.
    发明授权
    Methods and compositions for rapid in vitro propagation of Swertia chirata 有权
    Swertia chirata快速体外繁殖的方法和组成

    公开(公告)号:US06855547B2

    公开(公告)日:2005-02-15

    申请号:US10011208

    申请日:2001-11-30

    IPC分类号: A01H4/00 C12N5/02 C12N5/00

    CPC分类号: A01H4/005

    摘要: The present disclosure relates to methods and compositions for in vitro cultivation of species of Swertia, e.g. Swertia chirata (Ham.). The disclosure provides culture media comprising Murashige and Skoog (MS) basal culture medium, plant hormones preferably selected from the group consisting of benzyladenine (BAP), gibberellic acid (GA3), and auxins, and other additives, e.g. sucrose and agar. Preferably, auxins are selected from the group consisting of indole acetic acid (IAA), indole butyric acid (IBA), and naphthalene acetic acid (NAA). Individual plant hormone concentrations are preferably from about 0.5 mg/L to about 5.0 mg/L. The disclosure provides methods of in vitro cultivation of Swertia chirata comprising contacting preferably axillary bud and/or shoot apex explants with an initiation medium comprising a modified MS basal culture medium, BAP, IAA, IBA, and NAA to produce a primary explant, contacting the primary explant with a shoot propagation medium comprising, a modified MS basal culture medium, BAP, GA3, and IAA to produce a secondary explant, contacting a secondary explant with a rooting medium comprising a modified MS basal culture medium, IAA, IBA, and NAA. The methods and compositions of the invention are capable of inducing extraordinarily rapid in vitro propagation of Swertia chirata. The methods and compositions of the disclosure may be useful for conservation of this threatened species as well as producing bulk quantities, e.g. gram, kilogram or more, of plant material for medicinal applications.

    摘要翻译: 本公开涉及用于体外培养Swertia物种的方法和组合物,例如 芝士(火鸡)。 本公开提供包含Murashige和Skoog(MS)基础培养基的培养基,优选选自苄基腺嘌呤(BAP),赤霉酸(GA3)和生长素等的植物激素和其它添加剂,例如, 蔗糖和琼脂。 优选地,生长素选自吲哚乙酸(IAA),吲哚丁酸(IBA)和萘乙酸(NAA)。 个体植物激素浓度优选为约0.5mg / L至约5.0mg / L。 本公开提供了Swertia chirata的体外培养方法,其包括优选使腋芽和/或茎顶端外植体与包含修饰的MS基础培养基BAP,IAA,IBA和NAA的起始培养基接触以产生初级外植体, 初级外植体,其具有包含修饰的MS基础培养基,BAP,GA3和IAA的芽繁殖培养基,以产生第二外植体,使次生外植体与包含修饰的MS基础培养基,IAA,IBA和NAA的生根培养基接触 。 本发明的方法和组合物能够诱导Swertia chirata的异常快速的体外繁殖。 本公开的方法和组合物可用于保护该受威胁的物种以及产生大量的物质,例如, 克,千克或更多的药用植物材料。