利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

35 条记录 (耗时 0.033 秒)

    Methods and compositions for rapid in vitro propagation of Swertia chirata
    1.
    发明授权
    Methods and compositions for rapid in vitro propagation of Swertia chirata 有权
    Swertia chirata快速体外繁殖的方法和组成

    公开(公告)号：US07238527B2

    公开(公告)日：2007-07-03

    申请号：US10917919

    申请日：2004-08-13

    申请人： Ashok Ahuja ,  Sushma Koul ,  Brij Lal Kaul ,  Navin Kumar Verma ,  Mahraj Krishen Kaul ,  Ravinder Kumar Raina ,  Ghulam Nabi Qazi

    发明人： Ashok Ahuja ,  Sushma Koul ,  Brij Lal Kaul ,  Navin Kumar Verma ,  Mahraj Krishen Kaul ,  Ravinder Kumar Raina ,  Ghulam Nabi Qazi

    IPC分类号： C12N5/02

    CPC分类号： A01H4/005

    摘要： The present disclosure relates to methods and compositions for in vitro cultivation of species of Swertia, e.g. Swertia chirata (Ham.). The disclosure provides culture media comprising Murashige and Skoog (MS) basal culture medium, plant hormones preferably selected from the group consisting of benzyladenine (BAP), gibberellic acid (GA3), and auxins, and other additives, e.g. sucrose and agar. Preferably, auxins are selected from the group consisting of indole acetic acid (IAA), indole butyric acid (IBA), and naphthalene acetic acid (NAA). Individual plant hormone concentrations are preferably from about 0.5 mg/L to about 5.0 mg/L. The disclosure provides methods of in vitro cultivation of Swertia chirata comprising contacting preferably axillary bud and/or shoot apex explants with an initiation medium comprising a modified MS basal culture medium, BAP, IAA, IBA, and NAA to produce a primary explant, contacting the primary explant with a shoot propagation medium comprising, a modified MS basal culture medium, BAP, GA3, and IAA to produce a secondary explant, contacting a secondary explant with a rooting medium comprising a modified MS basal culture medium, IAA, IBA, and NAA. The methods and compositions of the invention are capable of inducing extraordinarily rapid in vitro propagation of Swertia chirata. The methods and compositions of the disclosure may be useful for conservation of this threatened species as well as producing bulk quantities, e.g. gram, kilogram or more, of plant material for medicinal

    摘要翻译： 本公开涉及用于体外培养Swertia物种的方法和组合物，例如 芝士（火鸡）。 本公开提供了包含Murashige和Skoog（MS）基础培养基的培养基，优选选自苄基腺嘌呤（BAP），赤霉酸（GA 3 N 3）和植物生长素的植物激素和其它添加剂 ，例如 蔗糖和琼脂。 优选地，生长素选自吲哚乙酸（IAA），吲哚丁酸（IBA）和萘乙酸（NAA）。 个体植物激素浓度优选为约0.5mg / L至约5.0mg / L。 本公开提供了Swertia chirata的体外培养方法，其包括优选使腋芽和/或茎顶端外植体与包含修饰的MS基础培养基BAP，IAA，IBA和NAA的起始培养基接触以产生初级外植体， 初级外植体，其具有包含修饰的MS基础培养基BAP，GA 3 N和IAA的芽繁殖培养基以产生第二外植体，使次生外植体与包含修饰的MS基础培养物的生根培养基接触 中等，IAA，IBA和NAA。 本发明的方法和组合物能够诱导Swertia chirata的异常快速的体外繁殖。 本公开的方法和组合物可用于保护该受威胁的物种以及产生大量的物质，例如， 克，千克以上的药用植物材料

    Methods and compositions for rapid in vitro propagation of Swertia chirata
    2.
    发明授权
    Methods and compositions for rapid in vitro propagation of Swertia chirata 有权
    Swertia chirata快速体外繁殖的方法和组成

    公开(公告)号：US06855547B2

    公开(公告)日：2005-02-15

    申请号：US10011208

    申请日：2001-11-30

    申请人： Ashok Ahuja ,  Sushma Koul ,  Brij Lal Kaul ,  Navin Kumar Verma ,  Mahrak Krishen Kaul ,  Ravinder Kumar Raina ,  Ghulam Nabi Qazi

    发明人： Ashok Ahuja ,  Sushma Koul ,  Brij Lal Kaul ,  Navin Kumar Verma ,  Mahrak Krishen Kaul ,  Ravinder Kumar Raina ,  Ghulam Nabi Qazi

    IPC分类号： A01H4/00 ,  C12N5/02 ,  C12N5/00

    CPC分类号： A01H4/005

    摘要： The present disclosure relates to methods and compositions for in vitro cultivation of species of Swertia, e.g. Swertia chirata (Ham.). The disclosure provides culture media comprising Murashige and Skoog (MS) basal culture medium, plant hormones preferably selected from the group consisting of benzyladenine (BAP), gibberellic acid (GA3), and auxins, and other additives, e.g. sucrose and agar. Preferably, auxins are selected from the group consisting of indole acetic acid (IAA), indole butyric acid (IBA), and naphthalene acetic acid (NAA). Individual plant hormone concentrations are preferably from about 0.5 mg/L to about 5.0 mg/L. The disclosure provides methods of in vitro cultivation of Swertia chirata comprising contacting preferably axillary bud and/or shoot apex explants with an initiation medium comprising a modified MS basal culture medium, BAP, IAA, IBA, and NAA to produce a primary explant, contacting the primary explant with a shoot propagation medium comprising, a modified MS basal culture medium, BAP, GA3, and IAA to produce a secondary explant, contacting a secondary explant with a rooting medium comprising a modified MS basal culture medium, IAA, IBA, and NAA. The methods and compositions of the invention are capable of inducing extraordinarily rapid in vitro propagation of Swertia chirata. The methods and compositions of the disclosure may be useful for conservation of this threatened species as well as producing bulk quantities, e.g. gram, kilogram or more, of plant material for medicinal applications.

    摘要翻译： 本公开涉及用于体外培养Swertia物种的方法和组合物，例如 芝士（火鸡）。 本公开提供包含Murashige和Skoog（MS）基础培养基的培养基，优选选自苄基腺嘌呤（BAP），赤霉酸（GA3）和生长素等的植物激素和其它添加剂，例如， 蔗糖和琼脂。 优选地，生长素选自吲哚乙酸（IAA），吲哚丁酸（IBA）和萘乙酸（NAA）。 个体植物激素浓度优选为约0.5mg / L至约5.0mg / L。 本公开提供了Swertia chirata的体外培养方法，其包括优选使腋芽和/或茎顶端外植体与包含修饰的MS基础培养基BAP，IAA，IBA和NAA的起始培养基接触以产生初级外植体， 初级外植体，其具有包含修饰的MS基础培养基，BAP，GA3和IAA的芽繁殖培养基，以产生第二外植体，使次生外植体与包含修饰的MS基础培养基，IAA，IBA和NAA的生根培养基接触 。 本发明的方法和组合物能够诱导Swertia chirata的异常快速的体外繁殖。 本公开的方法和组合物可用于保护该受威胁的物种以及产生大量的物质，例如， 克，千克或更多的药用植物材料。

    Methods and compositions for in vitro germination and propagation of polygonatum cirrhifolium royle
    4.
    发明授权
    Methods and compositions for in vitro germination and propagation of polygonatum cirrhifolium royle 有权
    用于体外萌发和繁殖的方法和组合物

    公开(公告)号：US06905876B2

    公开(公告)日：2005-06-14

    申请号：US09998573

    申请日：2001-11-16

    申请人： Ghulam Nabi Qazi ,  Surrinder Kumar Lattoo ,  Avtar Krishan Dhar ,  Paresh Purohit ,  Ravinder Kumar Raina ,  Rekha Sapru Dhar

    发明人： Ghulam Nabi Qazi ,  Surrinder Kumar Lattoo ,  Avtar Krishan Dhar ,  Paresh Purohit ,  Ravinder Kumar Raina ,  Rekha Sapru Dhar

    IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/04

    CPC分类号： C12N5/0025 ,  C12N5/04

    摘要： The present disclosure relates to methods and compositions for in vitro cultivation of species of Polygonatum, e.g. Polygonatum cirrhifolium Royle. The disclosure provides culture media comprising MS basal culture media and plant hormones, preferably selected from the group consisting of gibberellic acid (GA3), 6-benzyl-aminopurine (BAP), and naphthalene acetic acid (NAA). The disclosure provides methods of in vitro cultivation of Polygonatum comprising contacting Polygonatum seeds with a first medium comprising MS basal culture medium and GA3, upon emergence of a hypocotyl, transferring this primary explant to a second medium comprising MS basal culture medium, BAP, and NAA, and upon emergence of a first foliage leaf, transferring this secondary explant to a third medium comprising MS basal culture medium, BAP, NAA, and gibberellic acid (GA3). The methods and compositions of the disclosure are capable of inducing and/or supporting uniform germination in less than about 90 days, synchronized development of epicotyl, coleoptile, and radicle, termination of epicotyl dormancy and combinations thereof. The present disclosure relates to the novel culture medium compositions, said compositions comprising Murashige and Skoog (MS), a basal culture medium, varied concentrations of plant hormones, and other additives, leading to extraordinarily fast and synchronized in vitro induction of germination and release of epicotyl dormancy in Polygonatum cirrhifolium Royle, an endangered medicinal plant species and a method for faster in vitro propagation of Polygonatum cirrhifolium Royle.

    摘要翻译： 本公开涉及用于体外培养Poly属物种的方法和组合物，例如， 茯苓 本公开提供了包含MS基础培养基和植物激素的培养基，优选选自赤霉酸（GA 3 N 3），6-苄基 - 氨基嘌呤（BAP）和萘乙酸（NAA ）。 本公开内容提供了Polygonatum的体外培养方法，包括在出现下胚轴的情况下将Polygonatum种子与包含MS基础培养基和GA 3的第一培养基接触，将该初级外植体转移到包含MS的第二培养基 基础培养基，BAP和NAA，并且在出现第一叶叶时，将该次级外植体转移到包含MS基础培养基，BAP，NAA和赤霉酸（GA 3 N 3）的第三培养基中， 。 本公开的方法和组合物能够在少于约90天内诱导和/或支持均匀的发芽，同时发育上胚轴，胚芽鞘和胚根，终止上胚轴休眠及其组合。 本公开涉及新型培养基组合物，所述组合物包含Murashige和Skoog（MS），基础培养基，不同浓度的植物激素和其它添加剂，导致异常快速和同步的体外诱导萌发和释放 Poly in ium。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。

    Composition for early and profuse sporulation in fungi and a method thereof
    9.
    发明授权
    Composition for early and profuse sporulation in fungi and a method thereof 有权
    早期和大量真菌孢子的组成及其方法

    公开(公告)号：US06593127B1

    公开(公告)日：2003-07-15

    申请号：US10067132

    申请日：2002-02-04

    申请人： Rajinder Kumar Khajuria ,  Ram Vilas Parsad Sinha ,  Vijeshwar Verma ,  Ghulam Nabi Qazi ,  Sukhdev Swami Handa

    发明人： Rajinder Kumar Khajuria ,  Ram Vilas Parsad Sinha ,  Vijeshwar Verma ,  Ghulam Nabi Qazi ,  Sukhdev Swami Handa

    IPC分类号： C12N116

    CPC分类号： C12N3/00 ,  C12N1/14

    摘要： The present invention relates to a composition useful for early and profuse sporulation in fungi with about 100 fold increase in spore count within 48 hours of inoculation, said composition comprising molasses ranging between 5-20 g/L, Corn Steep Liquor (CSL) ranging between 10-25 g/L, Sodium chloride (NaCl) ranging between 5-15 g/L, calcium sulphate (CaSO4) ranging between 0.1-0.5 g/L, Potassium dihydrogen phosphate (KH2PO4) ranging between 0.001-0.01 g/L, Magnesium sulphate (MgSO4.7 H2O) ranging between 0.001-0.01 g/L, Copper sulphate (CuSO4) ranging between 0.001-0.0050 g/L, Ferrous sulphate (FeSO4) ranging between 0.0009-0.005 g/L, an anti-foam agent, and optionally solidifying agent and a method thereof.

    摘要翻译： 本发明涉及用于早期和大量真菌孢子成分的组合物，其接种48小时内孢子数量增加约100倍，所述组合物包含范围在5-20g / L之间的糖蜜，玉米eep酒（CSL）的范围介于 10-25g / L，氯化钠（NaCl）为5-15g / L，硫酸钙（CaSO 4）为0.1-0.5g / L，磷酸二氢钾（KH 2 PO 4）为0.001-0.01g / L， 范围在0.001-0.01g / L之间的硫酸镁（MgSO 4·7H 2 O），0.001-0.0050g / L范围内的硫酸铜（CuSO 4），0.0009-0.005g / L范围内的硫酸亚铁（FeSO 4），防沫剂 ，和任选的固化剂及其方法。