公开(公告)号：US5565347A

公开(公告)日：1996-10-15

申请号：US113985

申请日：1993-08-30

申请人： JoAnne J. Fillatti ,  Bruce R. Thomas

发明人： JoAnne J. Fillatti ,  Bruce R. Thomas

IPC分类号： C12N15/82 ,  C12N15/84 ,  C12N5/04 ,  A01H4/00

CPC分类号： C12N15/8205

摘要： Plant species are produced by cocultivation transformation of cotyledon shoot cultures with a foreign gene followed by regeneration of plants from transformed cells, thereby producing plants capable of expressing the foreign gene. Particularly, tomato shoot cultures are employed and are transformed employing a manipulated Agrobacterium transformation system, followed by regeneration of the transformed plant tissue into plants.

摘要翻译： 通过用外来基因的子叶培养物的共培养转化产生植物物种，随后从转化细胞再生植物，从而产生能够表达外源基因的植物。 特别地，使用番茄芽培养物并使用操纵的农杆菌转化系统进行转化，随后将转化的植物组织再生成植物。

公开(公告)号：US07045681B2

公开(公告)日：2006-05-16

申请号：US10274748

申请日：2002-10-18

申请人： Raymond L. Rodriguez ,  Bruce R. Thomas

发明人： Raymond L. Rodriguez ,  Bruce R. Thomas

IPC分类号： C12N15/82 ,  C12N15/90 ,  C12N15/29

CPC分类号： C12N9/2422 ,  C12N15/8234

摘要： The present invention relates to a method for producing a gene product by expressing a gene encoding said gene product in angiosperm host cells, which method comprises: a) constructing a vector expressible in angiosperm host cells, said vector comprising a promoter region derived from an amylase gene selected from SBAmyA, SBAmyB, SBAmyC genes or SBAmyD of the sugar beet and a gene encoding a desired gene product; b) transforming a compatible angiosperm host cell with said vector; c) cultivating the resulting transformant host cell to a sugar-depleted or sugar-free condition to promote the expression of said gene under the control of such promoter region; and d) recovering the product of the expressed gene. The sugar beet gene when incorporated into a dicot seed or plant has improved biological properties. The gene sequences and the products thereof are claimed.

摘要翻译： 本发明涉及通过在被子植物宿主细胞中表达编码所述基因产物的基因来生产基因产物的方法，该方法包括：a）构建在被子植物宿主细胞中可表达的载体，所述载体包含衍生自淀粉酶的启动子区 选自SBAmyA，SBAmyB，SBAmyC基因或甜菜的SBAmyD的基因和编码所需基因产物的基因; b）用所述载体转化相容的被子植物宿主细胞; c）将所得的转化体宿主细胞培养至无糖或无糖的条件，以促进所述基因在该启动子区的控制下的表达; 和d）回收表达基因的产物。 掺入双子叶植物种子或植物时的甜菜基因具有改善的生物学性质。 要求保护基因序列及其产物。