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1.发明授权DNA sequences capable of expressing foreign proteins and metabolites in dicotyledonous plants and cell culture 失效能够在双子叶植物和细胞培养物中表达外源蛋白质和代谢物的DNA序列公开(公告)号：US07045681B2
公开(公告)日：2006-05-16
申请号：US10274748
申请日：2002-10-18
CPC分类号： C12N9/2422 , C12N15/8234
摘要： The present invention relates to a method for producing a gene product by expressing a gene encoding said gene product in angiosperm host cells, which method comprises: a) constructing a vector expressible in angiosperm host cells, said vector comprising a promoter region derived from an amylase gene selected from SBAmyA, SBAmyB, SBAmyC genes or SBAmyD of the sugar beet and a gene encoding a desired gene product; b) transforming a compatible angiosperm host cell with said vector; c) cultivating the resulting transformant host cell to a sugar-depleted or sugar-free condition to promote the expression of said gene under the control of such promoter region; and d) recovering the product of the expressed gene. The sugar beet gene when incorporated into a dicot seed or plant has improved biological properties. The gene sequences and the products thereof are claimed.
摘要翻译： 本发明涉及通过在被子植物宿主细胞中表达编码所述基因产物的基因来生产基因产物的方法，该方法包括：a）构建在被子植物宿主细胞中可表达的载体，所述载体包含衍生自淀粉酶的启动子区 选自SBAmyA，SBAmyB，SBAmyC基因或甜菜的SBAmyD的基因和编码所需基因产物的基因; b）用所述载体转化相容的被子植物宿主细胞; c）将所得的转化体宿主细胞培养至无糖或无糖的条件，以促进所述基因在该启动子区的控制下的表达; 和d）回收表达基因的产物。 掺入双子叶植物种子或植物时的甜菜基因具有改善的生物学性质。 要求保护基因序列及其产物。
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公开(公告)号：US08686225B2
公开(公告)日：2014-04-01
申请号：US13449233
申请日：2012-04-17
CPC分类号： G01N33/57492 , A23K10/30 , A23K20/147 , A23L7/198 , A23L7/20 , A23L33/105 , A23L33/19 , A23V2002/00 , B82Y30/00 , C07K14/415 , C07K14/485 , C07K14/65 , C07K14/79 , C07K14/8125 , C12N9/0065 , C12N9/2462 , C12N15/8216 , C12N15/8222 , C12N15/8234 , C12N15/8257 , G01N33/57484 , G01N2333/4725 , G01N2500/02 , A23V2300/21
摘要： The invention is directed to blood proteins produced in monocot seeds and isolated therefrom for use in therapeutic compositions, as well as to methods of making these isolated blood proteins and to therapeutic compositions comprising them.
摘要翻译： 本发明涉及在单子叶植物中产生并从其分离用于治疗组合物中的血液蛋白质，以及制备这些分离的血液蛋白质的方法以及包含它们的治疗组合物。
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公开(公告)号：US06288303B1
公开(公告)日：2001-09-11
申请号：US09105390
申请日：1998-06-25
申请人： Raymond L. Rodriguez
发明人： Raymond L. Rodriguez
IPC分类号： C12N1529
CPC分类号： C12N15/8222 , C07K14/8125 , C12N9/244 , C12N15/8223 , C12N15/8227 , C12N15/8234 , C12N15/8237 , C12N15/8257 , C12N15/8282 , C12Y302/01006
摘要： A group of novel rice &bgr;-glucanase genes, identified as &bgr;-glucanases 2-9 (Gns 2-9), and the corresponding &bgr;-glucanase enzymes, are disclosed. The genes, and the gene promoters, are useful in a variety of transgenic monocot plants, for achieving increased plant resistance to fungal infection, improved growth characteristics, and high levels of expression of heterologous protein in various tissues obtained from the plants.
摘要翻译： 公开了一组鉴定为β-葡聚糖酶2-9（Gns 2-9）的新型水稻β-葡聚糖酶基因和相应的β-葡聚糖酶。 基因和基因启动子可用于各种转基因植物单体植物，用于实现增加的植物对真菌感染的抗性，改善的生长特性以及从植物获得的各种组织中异源蛋白质的高水平表达。
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公开(公告)号：US6127145A
公开(公告)日：2000-10-03
申请号：US23339
申请日：1998-02-13
CPC分类号： C12N15/8257 , C12N15/8234 , C12N15/8235
摘要： A method for producing .alpha..sub.1 -antitrypsin (AAT) in plant cells is disclosed. Monocot plant cells transformed with an AAT coding sequence are cultivated under conditions efficient for protein expression and secretion. Also disclosed are a codon-optimized AAT coding sequence that is efficiently translated in plant cell culture and a novel AAT protein having the glycosylation pattern characteristic of plant cells and suitable for therapeutic use in humans.
摘要翻译： 公开了一种在植物细胞中产生α1-抗胰蛋白酶（AAT）的方法。 用AAT编码序列转化的单子叶植物细胞在对蛋白质表达和分泌有效的条件下培养。 还公开了在植物细胞培养物中有效翻译的密码子优化的AAT编码序列和具有植物细胞的糖基化模式特征并且适合于人类治疗用途的新型AAT蛋白质。
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公开(公告)号：US07417178B2
公开(公告)日：2008-08-26
申请号：US10639835
申请日：2003-08-12
申请人： Ning Huang , Raymond L. Rodriguez , Frank E. Hagie
发明人： Ning Huang , Raymond L. Rodriguez , Frank E. Hagie
CPC分类号： G01N33/57492 , A23K10/30 , A23K20/147 , A23L7/198 , A23L7/20 , A23L33/105 , A23L33/19 , A23V2002/00 , B82Y30/00 , C07K14/415 , C07K14/485 , C07K14/65 , C07K14/79 , C07K14/8125 , C12N9/0065 , C12N9/2462 , C12N15/8216 , C12N15/8222 , C12N15/8234 , C12N15/8257 , G01N33/57484 , G01N2333/4725 , G01N2500/02 , A23V2300/21
摘要： The invention is directed to seed and seed extract compositions containing levels of a human milk protein between 3-40% or higher of the total protein weight of the soluble protein extractable from the seed. Also disclosed is a method of producing the seed with high levels of extractable human milk protein. The method includes transforming a monocotyledonous plant with a chimeric gene having a protein-coding sequence encoding a protein normally present in human milk under the control of a seed maturation-specific promoter. The method may further includes a leader DNA sequence encoding a monocot seed-specific transit sequence capable to target a linked milk protein to a storage body.
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公开(公告)号：US06680425B1
公开(公告)日：2004-01-20
申请号：US09450515
申请日：1999-11-29
申请人： Raymond L. Rodriguez
发明人： Raymond L. Rodriguez
IPC分类号： C12N504
CPC分类号： C12N9/2422 , C12N15/8238
摘要： A chimeric plant promoter which is responsive to sugar depletion or deprivation is disclosed. The promoter includes a promoter element and, carried in the element, a heterologous sequence from the rice Amy3D that is responsive to sugar depletion. Also disclosed is a plant transfecting vector containing the sequence, and plant cell transfected with the vector.
摘要翻译： 公开了对糖消耗或剥夺有反应的嵌合植物启动子。 启动子包括启动子元件，并且在元件中携带来自对糖消耗有反应的稻米Amy3D的异源序列。 还公开了含有该序列的植物转染载体和用载体转染的植物细胞。
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公开(公告)号：US4588689A
公开(公告)日：1986-05-13
申请号：US585752
申请日：1984-03-02
CPC分类号： C12N9/22 , Y10S435/91
摘要： A novel restriction endonuclease designated XcyI recognizes and cleaves the sequence 5'-C.dwnarw.CCGGG-3', where the arrow indicates the cleavage site. The enzyme may be obtained from Xanthamonas cyanopsidis.Xanthamonas cyanopsidis strain 13D5 was deposited at the American Type Culture Collection on Jan. 20, 1984, and granted accession No. 39587.
摘要翻译： 称为XcyI的新型限制性内切核酸酶识别并切割序列5'-C和darr＆CCGGG-3'，其中箭头表示切割位点。 该酶可以从蓝假单胞菌（Xanthamonas cyanopsidis）获得。 蓝假丝酵母菌株13D5于1984年1月20日保藏于美国典型培养物保藏中心，授予加拿社第39587号。
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公开(公告)号：US06991824B2
公开(公告)日：2006-01-31
申请号：US10077381
申请日：2002-02-14
申请人： Ning Huang , Raymond L. Rodriguez , Frank E. Hagie
发明人： Ning Huang , Raymond L. Rodriguez , Frank E. Hagie
IPC分类号： A21D2/26
CPC分类号： G01N33/57492 , A23K10/30 , A23K20/147 , A23L7/198 , A23L7/20 , A23L33/105 , A23L33/19 , A23V2002/00 , B82Y30/00 , C07K14/415 , C07K14/485 , C07K14/65 , C07K14/79 , C07K14/8125 , C12N9/0065 , C12N9/2462 , C12N15/8216 , C12N15/8222 , C12N15/8234 , C12N15/8257 , G01N33/57484 , G01N2333/4725 , G01N2500/02 , A23V2300/21
摘要： The invention is directed to food and food additive compositions comprising one or more human milk proteins produced in the seeds of a transgenic plant and methods of making the same. The invention is further directed to improved infant formula comprising such food supplement composition.
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公开(公告)号：US5888789A
公开(公告)日：1999-03-30
申请号：US460720
申请日：1995-06-02
申请人： Raymond L. Rodriguez
发明人： Raymond L. Rodriguez
CPC分类号： C12N15/8222 , C12N15/8234 , C12N15/8242 , C12N15/8257
摘要： This invention provides for the secretion of heterologous protein in plant systems. In particular, this invention provides for the production of heterologous proteins by malting of monocot plant seeds. The heterologous genes are expressed during germination of the seeds and isolated from a malt. Also disclosed are chimeric genes, vectors and methods relating to the present invention. Protein production by cell culture techniques is also described.
摘要翻译： 本发明提供了植物系统中异源蛋白质的分泌。 特别地，本发明提供了单子叶植物种子发芽的异源蛋白质的生产。 异种基因在种子发芽过程中表达，并从麦芽中分离出来。 还公开了与本发明相关的嵌合基因，载体和方法。 还描述了通过细胞培养技术的蛋白质生产。
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公开(公告)号：US5693506A
公开(公告)日：1997-12-02
申请号：US153563
申请日：1993-11-16
申请人： Raymond L. Rodriguez
发明人： Raymond L. Rodriguez
IPC分类号： A01H5/00 , C12N5/10 , C12N15/09 , C12N15/29 , C12N15/82 , C12P21/02 , A01H1/00 , A01H1/04 , C07H21/04
CPC分类号： C12N15/8222 , C12N15/8234 , C12N15/8242 , C12N15/8257
摘要： This invention provides for the secretion of heterologous protein in plant systems. In particular, this invention provides for the production of heterologous proteins in plant cultu1res and seeds. Where seeds are the source of the protein, the heterologous genes are expressed during germination and isolated from a malt.
摘要翻译： 本发明提供了植物系统中异源蛋白质的分泌。 特别地，本发明提供在植物培养物和种子中产生异源蛋白质。 当种子是蛋白质的来源时，异源基因在萌发期间表达并从麦芽中分离出来。
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