公开(公告)号：US09376463B2

公开(公告)日：2016-06-28

申请号：US14086621

申请日：2013-11-21

Applicant: CHRETO ApS

Inventor： Jan Kyhse-Andersen

IPC: C07K1/22

CPC classification number: C07K1/22

Abstract: The present invention relates to a process for purification of a target biomolecule, comprising the steps: (a) contacting (i) a target biomolecule, (ii) a dual affinity polypeptide, and (iii) a solid support comprising a catching ligand, wherein the ratio between the equilibrium dissociation constants of the dual affinity polypeptide, [KD,t/KD,s], is at least 100 at standard conditions; and (b) recovering the target biomolecule by elution.

Abstract translation: 本发明涉及目标生物分子的纯化方法，包括以下步骤：（a）使目标生物分子，（ii）双重亲和多肽和（iii）包含捕获配体的固体支持物接触，其中 双重亲和力多肽[KD，t / KD，s]的平衡解离常数之间的比例在标准条件下至少为100; 和（b）通过洗脱回收目标生物分子。

公开(公告)号：US20240001268A1

公开(公告)日：2024-01-04

申请号：US18254456

申请日：2021-12-13

Applicant: CHRETO ApS

Inventor： Jan Kyhse-Andersen ,  Lars Winther

IPC: B01D37/02 ,  B01D29/01

CPC classification number: B01D37/02 ,  B01D29/01 ,  B01D2201/085

Abstract: A system and a method for filtering a predetermined molecule entrained as a 3D-complex in a liquid comprising a filter forming solid all forming a filter forming suspension, the system having a filter chamber into which the filter forming suspension is fed and drained to form a filter with inclusions of the 3D-complex, where after a cleansing liquid is fed to a neighbouring liquid chamber and fed evenly through the filter to cleanse the filter and subsequently dissolve the complex to retrieve the product or target molecule.