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    Methods for increasing the production of ethanol from microbial fermentation
    4.
    发明授权
    Methods for increasing the production of ethanol from microbial fermentation 有权
    从微生物发酵中提高乙醇生产的方法

    公开(公告)号：US08574879B2

    公开(公告)日：2013-11-05

    申请号：US13325149

    申请日：2011-12-14

    申请人： James L. Gaddy ,  Dinesh K. Arora ,  Ching-Whan Ko ,  John Randall Phillips ,  Rahul Basu ,  Carl V. Wilkstrom ,  Edgar C. Clausen

    发明人： James L. Gaddy ,  Dinesh K. Arora ,  Ching-Whan Ko ,  John Randall Phillips ,  Rahul Basu ,  Carl V. Wilkstrom ,  Edgar C. Clausen

    IPC分类号： C12P7/06

    CPC分类号： C12P7/065 ,  C12M21/04 ,  C12M21/12 ,  C12M29/02 ,  C12M29/18 ,  C12M43/02 ,  Y02E50/17 ,  Y02E50/343

    摘要： A stable continuous method for producing ethanol from the anaerobic bacterial fermentation of a gaseous substrate containing at least one reducing gas involves culturing in a fermentation bioreactor anaerobic, acetogenic bacteria in a liquid nutrient medium; supplying the gaseous substrate to the bioreactor; and manipulating the bacteria in the bioreactor by reducing the redox potential, or increasing the NAD(P)H TO NAD(P) ratio, in the fermentation broth after the bacteria achieves a steady state and stable cell concentration in the bioreactor. The free acetic acid concentration in the bioreactor is maintained at less than 5 g/L free acid. This method allows ethanol to be produced in the fermentation broth in the bioreactor at a productivity of greater than 10 g/L per day. Both ethanol and acetate are produced in a ratio of ethanol to acetate ranging from 1:1 to 20:1.

    摘要翻译： 从含有至少一种还原气体的气态底物的厌氧细菌发酵生产乙醇的稳定的连续方法包括在液体营养培养基中的发酵生物反应器中厌氧发酵细菌中培养; 将气态基质供应到生物反应器; 并且在细菌在生物反应器中达到稳定状态和稳定的细胞浓度之后通过降低氧化还原电位或增加发酵液中的NAD（P）H TO NAD（P）比来操纵生物反应器中的细菌。 生物反应器中的游离乙酸浓度保持在低于5g / L游离酸。 该方法允许以每天大于10g / L的生产率在生物反应器中的发酵液中生产乙醇。 乙醇和乙酸乙酯均以乙醇与乙酸乙酯的比例为1：1至20：1生产。

    Methods for increasing the production of ethanol from microbial fermentation
    5.
    发明授权
    Methods for increasing the production of ethanol from microbial fermentation 有权
    从微生物发酵中提高乙醇生产的方法

    公开(公告)号：US08642301B2

    公开(公告)日：2014-02-04

    申请号：US13313136

    申请日：2011-12-07

    申请人： James L. Gaddy ,  Dinesh K. Arora ,  Ching-Whan Ko ,  John Randall Phillips ,  Rahul Basu ,  Carl V. Wilkstrom ,  Edgar C. Clausen

    发明人： James L. Gaddy ,  Dinesh K. Arora ,  Ching-Whan Ko ,  John Randall Phillips ,  Rahul Basu ,  Carl V. Wilkstrom ,  Edgar C. Clausen

    IPC分类号： C12P7/06

    CPC分类号： C12P7/065 ,  C12M21/04 ,  C12M21/12 ,  C12M29/02 ,  C12M29/18 ,  C12M43/02 ,  Y02E50/17 ,  Y02E50/343

    摘要： A stable continuous method for producing ethanol from the anaerobic bacterial fermentation of a gaseous substrate containing at least one reducing gas involves culturing in a fermentation bioreactor anaerobic, acetogenic bacteria in a liquid nutrient medium; supplying the gaseous substrate to the bioreactor; and manipulating the bacteria in the bioreactor by reducing the redox potential, or increasing the NAD(P)H TO NAD(P) ratio, in the fermentation broth after the bacteria achieves a steady state and stable cell concentration in the bioreactor. The free acetic acid concentration in the bioreactor is maintained at less than 5 g/L free acid. This method allows ethanol to be produced in the fermentation broth in the bioreactor at a productivity of greater than 10 g/L per day. Both ethanol and acetate are produced in a ratio of ethanol to acetate ranging from 1:1 to 20:1.

    摘要翻译： 从含有至少一种还原气体的气态底物的厌氧细菌发酵生产乙醇的稳定的连续方法包括在液体营养培养基中的发酵生物反应器中厌氧发酵细菌中培养; 将气态基质供应到生物反应器; 并且在细菌在生物反应器中达到稳定状态和稳定的细胞浓度之后通过降低氧化还原电位或增加发酵液中的NAD（P）H TO NAD（P）比来操纵生物反应器中的细菌。 生物反应器中的游离乙酸浓度保持在低于5g / L游离酸。 该方法允许以每天大于10g / L的生产率在生物反应器中的发酵液中生产乙醇。 乙醇和乙酸乙酯均以乙醇与乙酸乙酯的比例为1：1至20：1生产。