公开(公告)号：US08647851B2

公开(公告)日：2014-02-11

申请号：US13314862

申请日：2011-12-08

Applicant: James L. Gaddy ,  Dinesh K. Arora ,  Ching-Whan Ko ,  John Randall Phillips ,  Rahul Basu ,  Carl V. Wikstrom ,  Edgar C. Clausen

Inventor： James L. Gaddy ,  Dinesh K. Arora ,  Ching-Whan Ko ,  John Randall Phillips ,  Rahul Basu ,  Carl V. Wikstrom ,  Edgar C. Clausen

IPC: C12P7/06

CPC classification number: C12P7/065 ,  C12M21/04 ,  C12M21/12 ,  C12M29/02 ,  C12M29/18 ,  C12M43/02 ,  Y02E50/17 ,  Y02E50/343

Abstract: A stable continuous method for producing ethanol from the anaerobic bacterial fermentation of a gaseous substrate containing at least one reducing gas involves culturing in a fermentation bioreactor anaerobic, acetogenic bacteria in a liquid nutrient medium; supplying the gaseous substrate to the bioreactor; and manipulating the bacteria in the bioreactor by reducing the redox potential, or increasing the NAD(P)H TO NAD(P) ratio, in the fermentation broth after the bacteria achieves a steady state and stable cell concentration in the bioreactor. The free acetic acid concentration in the bioreactor is maintained at less than 5 g/L free acid. This method allows ethanol to be produced in the fermentation broth in the bioreactor at a productivity of greater than 10 g/L per day. Both ethanol and acetate are produced in a ratio of ethanol to acetate ranging from 1:1 to 20:1.

公开(公告)号：US08642301B2

公开(公告)日：2014-02-04

申请号：US13313136

申请日：2011-12-07

Applicant: James L. Gaddy ,  Dinesh K. Arora ,  Ching-Whan Ko ,  John Randall Phillips ,  Rahul Basu ,  Carl V. Wilkstrom ,  Edgar C. Clausen

Inventor： James L. Gaddy ,  Dinesh K. Arora ,  Ching-Whan Ko ,  John Randall Phillips ,  Rahul Basu ,  Carl V. Wilkstrom ,  Edgar C. Clausen

IPC: C12P7/06

CPC classification number: C12P7/065 ,  C12M21/04 ,  C12M21/12 ,  C12M29/02 ,  C12M29/18 ,  C12M43/02 ,  Y02E50/17 ,  Y02E50/343

Abstract: A stable continuous method for producing ethanol from the anaerobic bacterial fermentation of a gaseous substrate containing at least one reducing gas involves culturing in a fermentation bioreactor anaerobic, acetogenic bacteria in a liquid nutrient medium; supplying the gaseous substrate to the bioreactor; and manipulating the bacteria in the bioreactor by reducing the redox potential, or increasing the NAD(P)H TO NAD(P) ratio, in the fermentation broth after the bacteria achieves a steady state and stable cell concentration in the bioreactor. The free acetic acid concentration in the bioreactor is maintained at less than 5 g/L free acid. This method allows ethanol to be produced in the fermentation broth in the bioreactor at a productivity of greater than 10 g/L per day. Both ethanol and acetate are produced in a ratio of ethanol to acetate ranging from 1:1 to 20:1.

Abstract translation: 从含有至少一种还原气体的气态底物的厌氧细菌发酵生产乙醇的稳定的连续方法包括在液体营养培养基中的发酵生物反应器中厌氧发酵细菌中培养; 将气态基质供应到生物反应器; 并且在细菌在生物反应器中达到稳定状态和稳定的细胞浓度之后通过降低氧化还原电位或增加发酵液中的NAD（P）H TO NAD（P）比来操纵生物反应器中的细菌。 生物反应器中的游离乙酸浓度保持在低于5g / L游离酸。 该方法允许以每天大于10g / L的生产率在生物反应器中的发酵液中生产乙醇。 乙醇和乙酸乙酯均以乙醇与乙酸乙酯的比例为1：1至20：1生产。

公开(公告)号：US08518691B2

公开(公告)日：2013-08-27

申请号：US13302642

申请日：2011-11-22

Applicant: Shih-Perng Tsai ,  Rathin Datta ,  Rahul Basu ,  Seong-Hoon Yoon

Inventor： Shih-Perng Tsai ,  Rathin Datta ,  Rahul Basu ,  Seong-Hoon Yoon

IPC: C12M1/12 ,  C12M1/00

CPC classification number: C12P7/04 ,  C12M21/12 ,  C12M23/24 ,  C12M25/10 ,  C12M29/16 ,  Y02E50/17

Abstract: A membrane supported bioreactor arrangement and method for anaerobic conversion of gas into liquid products including membrane modules having hollow fibers, each of the hollow fibers formed from an asymmetric membrane wall having a porous outer layer defining biopores for retaining a porous biolayer about the outer surface of the membrane wall and a less permeable hydration layer around the hollow fiber lumen; a membrane vessel for retaining the membrane modules in a process gas for formation of the biolayer on the outer surface of the hollow fiber wall by interaction of microorganisms with a process gas and for the production of a liquid product, wherein the membrane vessel retains the membrane modules in a common horizontal plane; provides a seal between contents of the membrane tank and ambient atmosphere; and includes a liquid supply conduit for communicating the process liquid with the hollow fiber lumens of the hollow fibers.

Abstract translation: 一种用于将气体厌氧转化成液体产物的膜负载的生物反应器布置和方法，包括具有中空纤维的膜组件，每个中空纤维由不对称膜壁形成，所述不对称膜壁具有限定生物孔的多孔外层，所述多孔外层限定了围绕外部表面的多孔生物层 所述膜壁和围绕所述中空纤维内腔的较不透水的水合层; 膜容器，用于通过微生物与工艺气体的相互作用和液体产物的生产将膜组件保持在用于形成中空纤维壁的外表面上的生物层的工艺气体中，其中膜容器保留膜 模块在共同的水平面; 提供膜罐内部和周围环境之间的密封; 并且包括用于使处理液体与中空纤维的中空纤维流体连通的液体供应管道。

公开(公告)号：US08212093B2

公开(公告)日：2012-07-03

申请号：US12468695

申请日：2009-05-19

Applicant: Rathin Datta ,  Richard E. Tobey ,  Rahul Basu

Inventor： Rathin Datta ,  Richard E. Tobey ,  Rahul Basu

IPC: C07C4/00 ,  C12M1/12 ,  C12P7/08

CPC classification number: C12P7/06 ,  C07C1/24 ,  C12P7/065 ,  Y02E50/17 ,  C07C11/04

Abstract: The production of feed for an olefin hydration zone is improved by integrating treatment of an alcohol containing stream from a fermentation zone into an alcohol separation section. The process passes a stream comprising alcohol, water and an organic acid to a separation column. The separation column concetrates the alcohol and organic acids into an upper column fraction. An additive for neutralization the organic acid into contact with said upper column fraction and reacts with the organic acid to produce a neutralization product that passes to a lower portion of the separation column. The column provides an overhead stream for an olefin dehydration zone having an increased concentration of alcohol and a reduced concentration of organic acid. A column bottoms stream containing the neutralization product returns as an input stream to supply the neutralization product to a fermentation zone that produces the alcohol containing stream.

Abstract translation: 通过将来自发酵区的含酒精流的处理整合到醇分离部分来改进烯烃水合区的饲料的生产。 该方法将包含醇，水和有机酸的物流通过分离塔。 分离柱将醇和有机酸混合成上层馏分。 用于中和有机酸与所述上层馏分接触的添加剂，并与有机酸反应以产生通过分离塔下部的中和产物。 该柱为具有增加的醇浓度和降低的有机酸浓度的烯烃脱水区提供塔顶流。 含有中和产物的塔底流返回作为输入流，以将中和产物供应到产生含酒精流的发酵区。

公开(公告)号：US20120088284A1

公开(公告)日：2012-04-12

申请号：US13331192

申请日：2011-12-20

Applicant: James L. Gaddy ,  Dinesh K. Arora ,  Ching-Whan Ko ,  John Randall Phillips ,  Rahul Basu ,  Carl V. Wilkstrom ,  Edgar C. Clausen

Inventor： James L. Gaddy ,  Dinesh K. Arora ,  Ching-Whan Ko ,  John Randall Phillips ,  Rahul Basu ,  Carl V. Wilkstrom ,  Edgar C. Clausen

IPC: C12P7/14

CPC classification number: C12P7/065 ,  C12M21/04 ,  C12M21/12 ,  C12M29/02 ,  C12M29/18 ,  C12M43/02 ,  Y02E50/343

Abstract: A stable continuous method for producing ethanol from anaerobic fermentation of a gaseous substrate containing at least one reducing gas in a liquid nutrient medium; supplying the gaseous substrate to the bioreactor. The free acetic acid concentration in the bioreactor is maintained at less than 5 g/L free acid. This method allows ethanol to be produced in the fermentation broth in the bioreactor at a productivity of greater than 10 g/L per day. Both ethanol and acetate are produced in a ratio of ethanol to acetate ranging from 1:1 to 20:1.

公开(公告)号：US20120077240A1

公开(公告)日：2012-03-29

申请号：US13313136

申请日：2011-12-07

Applicant: James L. Gaddy ,  Dinesh K. Arora ,  Ching-Whan Ko ,  John Randall Phillips ,  Rahul Basu ,  Carl V. Wilkstrom ,  Edgar C. Clausen

Inventor： James L. Gaddy ,  Dinesh K. Arora ,  Ching-Whan Ko ,  John Randall Phillips ,  Rahul Basu ,  Carl V. Wilkstrom ,  Edgar C. Clausen

IPC: C12P7/06

CPC classification number: C12P7/065 ,  C12M21/04 ,  C12M21/12 ,  C12M29/02 ,  C12M29/18 ,  C12M43/02 ,  Y02E50/17 ,  Y02E50/343

Abstract: A stable continuous method for producing ethanol from the anaerobic bacterial fermentation of a gaseous substrate containing at least one reducing gas involves culturing in a fermentation bioreactor anaerobic, acetogenic bacteria in a liquid nutrient medium; supplying the gaseous substrate to the bioreactor; and manipulating the bacteria in the bioreactor by reducing the redox potential, or increasing the NAD(P)H TO NAD(P) ratio, in the fermentation broth after the bacteria achieves a steady state and stable cell concentration in the bioreactor. The free acetic acid concentration in the bioreactor is maintained at less than 5 g/L free acid. This method allows ethanol to be produced in the fermentation broth in the bioreactor at a productivity of greater than 10 g/L per day. Both ethanol and acetate are produced in a ratio of ethanol to acetate ranging from 1:1 to 20:1.

公开(公告)号：US08062873B2

公开(公告)日：2011-11-22

申请号：US12258162

申请日：2008-10-24

Applicant: Shih-Perng Tsai ,  Rathin Datta ,  Rahul Basu ,  Seong-Hoon Yoon

Inventor： Shih-Perng Tsai ,  Rathin Datta ,  Rahul Basu ,  Seong-Hoon Yoon

IPC: C12M1/12 ,  C12M1/14 ,  C12P1/04

CPC classification number: C12P7/04 ,  C12M21/12 ,  C12M23/24 ,  C12M25/10 ,  C12M29/16 ,  Y02E50/17

Abstract: A membrane supported bioreactor arrangement and method for anerobic conversion of gas into liquid products including membrane modules having hollow fibers, each of the hollow fibers formed from an asymmetric membrane wall having a porous outer layer defining biopores for retaining a porous biolayer about the outer surface of the membrane wall and a less permeable hydration layer around the hollow fiber lumen; a membrane vessel for retaining the membrane modules in a process gas for formation of the biolayer on the outer surface of the hollow fiber wall by interaction of microorganisms with a process gas and for the production of a liquid product, wherein the membrane vessel retains the membrane modules in a common horizontal plane; provides a seal between contents of the membrane tank and ambient atmosphere; and includes a liquid supply conduit for communicating the process liquid with the hollow fiber lumens of the hollow fibers.

Abstract translation: 一种膜支持的生物反应器装置和方法，用于将气体厌氧转化成液体产物，包括具有中空纤维的膜组件，每个中空纤维由不对称膜壁形成，所述不对称膜壁具有限定生物孔的多孔外层，所述多孔外层限定了围绕外部表面的多孔生物层 所述膜壁和围绕所述中空纤维内腔的较不透水的水合层; 膜容器，用于通过微生物与工艺气体的相互作用和液体产物的生产将膜组件保持在用于形成中空纤维壁的外表面上的生物层的工艺气体中，其中膜容器保留膜 模块在共同的水平面; 提供膜罐内部和周围环境之间的密封; 并且包括用于使处理液体与中空纤维的中空纤维流体连通的液体供应管道。

公开(公告)号：US07923227B2

公开(公告)日：2011-04-12

申请号：US12574499

申请日：2009-10-06

Applicant: Robert Hickey ,  Rahul Basu ,  Rathin Datta ,  Shih-Perng Tsai

Inventor： Robert Hickey ,  Rahul Basu ,  Rathin Datta ,  Shih-Perng Tsai

IPC: C12P7/06

CPC classification number: C12M29/16 ,  C12M21/12 ,  C12M25/10 ,  C12P7/04 ,  Y02E50/17

Abstract: A stable system for producing liquid products such as ethanol, butanol and other chemicals from syngas components contacts CO or a mixture of CO2 and H2 with a hydrophilic membrane under anaerobic conditions and transfers these components into contact with microorganisms contained as a biofilm on the membrane. Maintaining the microorganisms as a biolayer on the surface of the membrane facilitates cleaning of the membrane surface that retains the biofilm. In addition the shell gas space that surrounds the membranes may be flooded to reduce or remove the biofilm. Agitation of the liquid, by for example the bubbling of gas in the surrounding shell space, can fully or partially remove the biofilm from the membrane.

Abstract translation: 用于从合成气组分生产液体产物如乙醇，丁醇和其它化学品的稳定系统在厌氧条件下接触CO或亲水膜的CO或CO 2和H 2的混合物，并将这些组分转移到膜上作为生物膜的微生物接触。 将微生物作为生物层保持在膜的表面上有助于清洁保留生物膜的膜表面。 此外，围绕膜的壳体气体空间可能被淹没以减少或去除生物膜。 液体的搅拌，例如气体在周围的壳体空间中鼓泡，可以完全或部分地从膜中除去生物膜。

公开(公告)号：US20090017514A1

公开(公告)日：2009-01-15

申请号：US12205540

申请日：2008-09-05

Applicant: Rathin Datta ,  Shih-Perng Tsai ,  Rahul Basu ,  Seong-Hoon Yoon

Inventor： Rathin Datta ,  Shih-Perng Tsai ,  Rahul Basu ,  Seong-Hoon Yoon

IPC: C12P1/04 ,  C12M1/04

CPC classification number: C12P7/04 ,  C12M21/12 ,  C12M23/44 ,  C12M25/10 ,  C12M29/16 ,  Y02E50/17

Abstract: Ethanol and other liquid products are produced by contacting syngas components such as CO or a mixture of CO2 and H2 with a surface of a membrane under anaerobic conditions and transferring these components in contact with a biofilm on the opposite side of the membrane. These steps provide a stable system for producing liquid products such as ethanol, butanol and other chemicals. The gas fed on the membrane's gas contact side transports through the membrane to form a biofilm of anaerobic microoganisms that converted the syngas to desired liquid products. A liquid impermeable layer of the membrane assists in establishing direct gas phase contact syngas components with the microorganisms. The system can sustain production with a variety of microorganisms and membrane configurations.

Abstract translation: 乙醇和其它液体产物通过将合成气组分如CO或CO 2和H 2的混合物与膜的表面在厌氧条件下接触而生产，并将这些组分与膜的相反侧上的生物膜接触。 这些步骤为生产液体产品如乙醇，丁醇和其他化学品提供了稳定的系统。 供应在膜的气体接触侧的气体输送通过膜以形成将合成气转化为所需液体产物的厌氧微生物的生物膜。 膜的液体不可渗透层有助于与微生物建立直接的气相接触合成气成分。 该系统可以通过各种微生物和膜结构来维持生产。

公开(公告)号：US08222026B2

公开(公告)日：2012-07-17

申请号：US12258180

申请日：2008-10-24

Applicant: Shih-Perng Tsai ,  Rathin Datta ,  Rahul Basu ,  Seong-Hoon Yoon

Inventor： Shih-Perng Tsai ,  Rathin Datta ,  Rahul Basu ,  Seong-Hoon Yoon

IPC: C12M1/12 ,  C12M1/00 ,  C12M3/00

CPC classification number: C12P7/04 ,  C12M21/12 ,  C12M23/24 ,  C12M25/10 ,  C12M29/16 ,  Y02E50/17

Abstract: A membrane supported bioreactor arrangement and method for anaerobic conversion of gas into liquid products including membrane modules having hollow fibers packed across a cross sectional area of the membrane module, each of the hollow fibers formed from an asymmetric membrane wall having a porous outer layer defining biopores for retaining a porous biolayer about the outer surface of the membrane wall and a less permeable hydration layer around the hollow fiber lumen; a membrane vessel for surrounding the outside of the hollow fibers with a process gas from a gas supply conduit; and a liquid supply conduit operably connected to the hollow fibers for supplying a process liquid to the hollow fiber lumens. The gas supply conduit enables the formation of a biolayer on the outer surface of the hollow fiber wall by interaction of microorganisms with the process gas and the production of a liquid product.

Abstract translation: 一种膜支持的生物反应器装置和用于将气体厌氧转化成液体产物的方法，包括膜组件的膜组件，膜组件横跨膜组件的横截面区域填充，每个中空纤维由具有限定生物孔的多孔外层的不对称膜壁 用于保持围绕所述膜壁的外表面的多孔生物层和围绕所述中空纤维腔的较不透水的水合层; 用于利用来自气体供应管道的处理气体围绕中空纤维外部的膜容器; 以及可操作地连接到中空纤维上的液体供应管道，用于将处理液体供应到中空纤维内腔。 气体供应导管能够通过微生物与工艺气体的相互作用和液体产物的生产在中空纤维壁的外表面上形成生物层。