公开(公告)号：US06482622B1

公开(公告)日：2002-11-19

申请号：US09645707

申请日：2000-08-24

申请人： Joel Robert Cherry ,  Allan Svendsen ,  Carsten Denmark Andersen ,  Lars Beier ,  Torben Peter Frandsen ,  Thomas Schäfer

发明人： Joel Robert Cherry ,  Allan Svendsen ,  Carsten Denmark Andersen ,  Lars Beier ,  Torben Peter Frandsen ,  Thomas Schäfer

IPC分类号： C12N900

CPC分类号： C12N9/2417 ,  A21D8/042

摘要： The inventors have discovered some striking, and not previously predicted structural similarities and differences between the structure of Novamyl and the reported structures of CGTases, and based on this they have constructed variants of maltogenic alpha-amylase having CGTase activity and variants of CGTase having maltogenic alpha-amylase activity. Further, on the basis of sequence homology between Novamyl® and CGTases, the inventors have constructed hybrid enzymes with one or more improvements to specific properties of the parent enzymes, using recombinant DNA methodology.

摘要翻译： 发明人已经发现了一些引人注目的，并没有预先结合的Novamyl结构与报道的CGTase结构之间的结构相似性和差异，并且基于此，它们构建了具有CGT酶活性的麦芽糖α-淀粉酶变异体和具有麦芽糖α 淀粉酶活性。 此外，基于Novamyl和CGTases之间的序列同源性，发明人使用重组DNA方法构建了对母体酶的特定性质具有一个或多个改进的杂合酶。

公开(公告)号：US20090142803A1

公开(公告)日：2009-06-04

申请号：US12208425

申请日：2008-09-11

申请人： Joel Robert Cherry ,  Allan Svendsen ,  Carsten Denmark Andersen ,  Lars Beier ,  Torben Peter Frandsen ,  Thomas Schafer

发明人： Joel Robert Cherry ,  Allan Svendsen ,  Carsten Denmark Andersen ,  Lars Beier ,  Torben Peter Frandsen ,  Thomas Schafer

IPC分类号： C12N9/10 ,  C12P21/00

CPC分类号： C12N9/2417 ,  A21D8/042

摘要： The inventors have discovered some striking, and not previously predicted structural similarities and differences between the structure of Novamyl and the reported structures of CGTases, and based on this they have constructed variants of maltogenic alpha-amylase having CGTase activity and variants of CGTase having maltogenic alpha-amylase activity. Further, on the basis of sequence homology between Novamyl® and CGTases, the inventors have constructed hybrid enzymes with one or more improvements to specific properties of the parent enzymes, using recombinant DNA methodology.

摘要翻译： 发明人已经发现了一些引人注目的，并没有预先结合的Novamyl结构与报道的CGTase结构之间的结构相似性和差异，并且基于此，它们构建了具有CGT酶活性的麦芽糖α-淀粉酶变异体和具有麦芽糖α 淀粉酶活性。 此外，基于Novamyl和CGTases之间的序列同源性，本发明人使用重组DNA方法构建了对母体酶的特定性质具有一个或多个改进的杂合酶。