公开(公告)号：US07122792B2

公开(公告)日：2006-10-17

申请号：US11172554

申请日：2005-06-30

Applicant: Yu-Chie Chen ,  Cheng-Tai Chen ,  Ya-Shiuan Lin

Inventor： Yu-Chie Chen ,  Cheng-Tai Chen ,  Ya-Shiuan Lin

IPC: H01J49/00 ,  B01D59/44

CPC classification number: H01J49/164

Abstract: The invention provides a simple, rapid and cost-effective metal oxide-assisted laser desorption/ionization mass spectrometry (MOALDI MS) without the addition of light-absorbing organic-matrix, comprising the use of (a) an inorganic metal oxide with light absorbing capability as an assisting material to render desorption/ionization of samples in laser desorption/ionization mass spectrometry and (b) a citric acid buffer as the proton source for enhancing the ionization efficiency for analytes. Metal oxide assisting materials is not only restricted to the uses of films. Metal oxide nanoparticles are also suitable to be used as the assisting materials. Low matrix background, stable surface feature, homogeneous sample deposition, and wide detectable mass range are the merits of MOALDI MS.

Abstract translation: 本发明提供了不添加光吸收有机基质的简单，快速且具有成本效益的金属氧化物辅助激光解吸/电离质谱（MOALDI MS），其包括使用（a）无机金属氧化物与光吸收 作为辅助材料的能力，以在激光解吸/电离质谱中进行样品的解吸/电离，以及（b）柠檬酸缓冲液作为提高分析物的电离效率的质子源。 金属氧化物辅助材料不仅限于膜的用途。 金属氧化物纳米颗粒也适合用作辅助材料。 低基质背景，稳定的表面特征，均匀的样品沉积和广泛的可检测质量范围是MOALDI MS的优点。

公开(公告)号：US20100184962A1

公开(公告)日：2010-07-22

申请号：US12591325

申请日：2009-11-17

Applicant: Yu-Chie Chen ,  Cheng-Tai Chen ,  Wei-Yu Chen ,  Chun-Yuen Lo ,  Hong-Yi Lin ,  Chih Chi Liu

Inventor： Yu-Chie Chen ,  Cheng-Tai Chen ,  Wei-Yu Chen ,  Chun-Yuen Lo ,  Hong-Yi Lin ,  Chih Chi Liu

IPC: C07K1/14

CPC classification number: G01N33/552 ,  B01J13/04 ,  B01J13/22 ,  B82Y25/00 ,  B82Y30/00 ,  C01G49/02 ,  C01P2004/64 ,  C01P2004/84 ,  C01P2006/42 ,  C04B35/62805 ,  C04B35/62807 ,  C04B35/62813 ,  C04B35/62818 ,  C04B35/62894 ,  C04B2235/3272 ,  C04B2235/441 ,  G01N33/551 ,  G01N33/6851 ,  H01F1/0054 ,  H01F1/36

Abstract: The present invention relates to a metal oxide nano-composite magnetic material, fabrication method, and method for linkage, enrichment, and isolation of phosphorylated species. The metal oxide nano-composite magnetic material comprises the magnetic iron oxide nanoparticle, a silica layer immobilized onto the magnetic iron oxide nanoparticle and a metal oxide layer coated onto the silica layer. The magnetic iron oxide nanoparticles can be used for absorbing microwave radiation to accelerate the enrichment and linkage for phosphorylated species onto the metal oxide nano-composite magnetic material. Furthermore, the magnetic property of magnetic iron oxide nanoparticles leads to isolation of the metal oxide nano-composite magnetic material-target species conjugates by simply employing an external magnetic field. The specificity of the metal oxide on the surface of the metal oxide nano-composite magnetic material for phosphorylated species also leads to the linkage/enrichment of phosphorylated species with high selectivity.

Abstract translation: 本发明涉及金属氧化物纳米复合磁性材料，制造方法和磷酸化物质的连接，富集和分离方法。 金属氧化物纳米复合磁性材料包括磁性氧化铁纳米颗粒，固定在磁性氧化铁纳米颗粒上的二氧化硅层和涂覆在二氧化硅层上的金属氧化物层。 磁性氧化铁纳米颗粒可用于吸收微波辐射，以加速磷酸化物质在金属氧化物纳米复合磁性材料上的富集和连接。 此外，磁性氧化铁纳米颗粒的磁性能通过简单地使用外部磁场来分离金属氧化物纳米复合磁性材料 - 靶物质共轭物。 金属氧化物对磷酸化物质的金属氧化物纳米复合磁性材料的表面的特异性也导致了高选择性的磷酸化物质的连接/富集。

公开(公告)号：US20090050839A1

公开(公告)日：2009-02-26

申请号：US12101451

申请日：2008-04-11

Applicant: YU-CHIE CHEN ,  CHENG-TAI CHEN ,  WEI-YU CHEN ,  CHUN-YUEN LO ,  HONG-YI LIN ,  CHIH CHI LIU

Inventor： YU-CHIE CHEN ,  CHENG-TAI CHEN ,  WEI-YU CHEN ,  CHUN-YUEN LO ,  HONG-YI LIN ,  CHIH CHI LIU

IPC: H01F1/01 ,  B05D5/00

CPC classification number: G01N33/552 ,  B01J13/04 ,  B01J13/22 ,  B82Y25/00 ,  B82Y30/00 ,  C01G49/02 ,  C01P2004/64 ,  C01P2004/84 ,  C01P2006/42 ,  C04B35/62805 ,  C04B35/62807 ,  C04B35/62813 ,  C04B35/62818 ,  C04B35/62894 ,  C04B2235/3272 ,  C04B2235/441 ,  G01N33/551 ,  G01N33/6851 ,  H01F1/0054 ,  H01F1/36

Abstract: The present invention relates to a metal oxide nano-composite magnetic material, fabrication method, and method for linkage, enrichment, and isolation of phosphorylated species. The metal oxide nano-composite magnetic material comprises the magnetic iron oxide nanoparticle, a silica layer immobilized onto the magnetic iron oxide nanoparticle and a metal oxide layer coated onto the silica layer. The magnetic iron oxide nanoparticles can be used for absorbing microwave radiation to accelerate the enrichment and linkage for phosphorylated species onto the metal oxide nano-composite magnetic material. Furthermore, the magnetic property of magnetic iron oxide nanoparticles leads to isolation of the metal oxide nano-composite magnetic material-target species conjugates by simply employing an external magnetic field. The specificity of the metal oxide on the surface of the metal oxide nano-composite magnetic material for phosphorylated species also leads to the linkage/enrichment of phosphorylated species with high selectivity in a short time (extracting time is 30˜90 seconds).

Abstract translation: 本发明涉及金属氧化物纳米复合磁性材料，制造方法和磷酸化物质的连接，富集和分离方法。 金属氧化物纳米复合磁性材料包括磁性氧化铁纳米颗粒，固定在磁性氧化铁纳米颗粒上的二氧化硅层和涂覆在二氧化硅层上的金属氧化物层。 磁性氧化铁纳米颗粒可用于吸收微波辐射，以加速磷酸化物质在金属氧化物纳米复合磁性材料上的富集和连接。 此外，磁性氧化铁纳米颗粒的磁性能通过简单地使用外部磁场来分离金属氧化物纳米复合磁性材料 - 靶物质共轭物。 金属氧化物对磷酸化物质的金属氧化物纳米复合磁性材料表面的特异性也导致磷酸化物质在短时间内的高选择性（提取时间为30〜90秒）的连接/富集。

公开(公告)号：US08735176B2

公开(公告)日：2014-05-27

申请号：US12101451

申请日：2008-04-11

Applicant: Yu-Chie Chen ,  Cheng-Tai Chen ,  Wei-Yu Chen ,  Chun-Yuen Lo ,  Hong-Yi Lin ,  Chih-Chi Liu

Inventor： Yu-Chie Chen ,  Cheng-Tai Chen ,  Wei-Yu Chen ,  Chun-Yuen Lo ,  Hong-Yi Lin ,  Chih-Chi Liu

IPC: G01N33/552 ,  G01N33/551

CPC classification number: G01N33/552 ,  B01J13/04 ,  B01J13/22 ,  B82Y25/00 ,  B82Y30/00 ,  C01G49/02 ,  C01P2004/64 ,  C01P2004/84 ,  C01P2006/42 ,  C04B35/62805 ,  C04B35/62807 ,  C04B35/62813 ,  C04B35/62818 ,  C04B35/62894 ,  C04B2235/3272 ,  C04B2235/441 ,  G01N33/551 ,  G01N33/6851 ,  H01F1/0054 ,  H01F1/36

Abstract: The present invention relates to a metal oxide nano-composite magnetic material, fabrication method, and method for linkage, enrichment, and isolation of phosphorylated species. The metal oxide nano-composite magnetic material comprises the magnetic iron oxide nanoparticle, a silica layer immobilized onto the magnetic iron oxide nanoparticle and a metal oxide layer coated onto the silica layer. The magnetic iron oxide nanoparticles can be used for absorbing microwave radiation to accelerate the enrichment and linkage for phosphorylated species onto the metal oxide nano-composite magnetic material. Furthermore, the magnetic property of magnetic iron oxide nanoparticles leads to isolation of the metal oxide nano-composite magnetic material-target species conjugates by simply employing an external magnetic field. The specificity of the metal oxide on the surface of the metal oxide nano-composite magnetic material for phosphorylated species also leads to the linkage/enrichment of phosphorylated species with high selectivity.

Abstract translation: 本发明涉及金属氧化物纳米复合磁性材料，制造方法和磷酸化物质的连接，富集和分离方法。 金属氧化物纳米复合磁性材料包括磁性氧化铁纳米颗粒，固定在磁性氧化铁纳米颗粒上的二氧化硅层和涂覆在二氧化硅层上的金属氧化物层。 磁性氧化铁纳米颗粒可用于吸收微波辐射，以加速磷酸化物质在金属氧化物纳米复合磁性材料上的富集和连接。 此外，磁性氧化铁纳米颗粒的磁性能通过简单地使用外部磁场来分离金属氧化物纳米复合磁性材料 - 靶物质共轭物。 金属氧化物对磷酸化物质的金属氧化物纳米复合磁性材料的表面的特异性也导致了高选择性的磷酸化物质的连接/富集。

公开(公告)号：US20060145068A1

公开(公告)日：2006-07-06

申请号：US11172554

申请日：2005-06-30

Applicant: Yu-Chie Chen ,  Cheng-Tai Chen ,  Ya-Shiuan Lin

Inventor： Yu-Chie Chen ,  Cheng-Tai Chen ,  Ya-Shiuan Lin

IPC: H01J49/16

CPC classification number: H01J49/164

Abstract: The invention provides a simple, rapid and cost-effective metal oxide-assisted laser desorption/ionization mass spectrometry (MOALDI MS) without the addition of light-absorbing organic-matrix, comprising the use of (a) an inorganic metal oxide with light absorbing capability as an assisting material to render desorption/ionization of samples in laser desorption/ionization mass spectrometry and (b) a citric acid buffer as the proton source for enhancing the iorization efficiency for analytes. Metal oxide assisting materials is not only restricted to the uses of films. Metal oxide nanoparticles are also suitable to be used as the assisting materials. Low matrix background, stable surface feature, homogeneous sample deposition, and wide detectable mass range are the merits of MOALDI MS.

Abstract translation: 本发明提供了不添加光吸收有机基质的简单，快速且具有成本效益的金属氧化物辅助激光解吸/电离质谱（MOALDI MS），其包括使用（a）无机金属氧化物与光吸收 作为辅助材料的能力，以在激光解吸/电离质谱中进行样品的解吸/电离，以及（b）柠檬酸缓冲液作为提高分析物的分解效率的质子源。 金属氧化物辅助材料不仅限于膜的用途。 金属氧化物纳米颗粒也适合用作辅助材料。 低基质背景，稳定的表面特征，均匀的样品沉积和广泛的可检测质量范围是MOALDI MS的优点。