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公开(公告)号：US08347411B2
公开(公告)日：2013-01-01
申请号：US12306928
申请日：2006-07-14
IPC分类号： G01Q80/00
摘要： The scanning probe microscope has a primary control loop (7, 11, 12) for keeping the phase and/or amplitude of deflection at constant values as well as a secondary control loop (9) that e.g. keeps the frequency of the cantilever oscillation constant by applying a suitable DC voltage to the probe while, at the same time, a conservative AC excitation is applied thereto. By actively controlling the frequency with the first control loop (7, 11, 12) and subsequently controlling the DC voltage in order to keep the frequency constant, a fast system is created that allows to determine the contact potential difference or a related property of the sample (3) quickly.
摘要翻译： 扫描探针显微镜具有用于将偏转的相位和/或幅度保持在恒定值的主控制回路（7,11,12）以及例如第二控制回路（9）。 通过向探针施加适当的直流电压来保持悬臂振荡的频率恒定，同时对其施加保守的AC激励。 通过利用第一控制回路（7,11,12）主动地控制频率并随后控制直流电压以保持频率恒定，产生快速系统，其允许确定接触电位差或相关属性 样品（3）快速。
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2.发明授权Transformed bryophyte cell having disrupted endogenous alpha 1,3-fucosyl and beta 1,2-xylosyl transferase encoding nucleotide sequences for the production of heterologous glycosylated proteins 有权转化的苔藓植物细胞破坏内源性α1,3-岩藻糖基和β1.2木糖基转移酶编码核苷酸序列，用于产生异源糖基化蛋白公开(公告)号：US07781197B2
公开(公告)日：2010-08-24
申请号：US10539725
申请日：2003-12-18
申请人： Ralf Reski , Gilbert Gorr , Eva Decker , Christian Stemmer , Otmar Lienhart , Anna Koprivova
发明人： Ralf Reski , Gilbert Gorr , Eva Decker , Christian Stemmer , Otmar Lienhart , Anna Koprivova
CPC分类号： C12N9/1051 , C12N5/04 , C12N9/1077 , C12N15/8257 , C12P21/005
摘要： Bryophyte plants and bryophyte plant cells comprising dysfunctional fucT and xylT genes and an introduced glycosyltransferase gene, methods for the production of glycosylated proteins therewith, vectors and uses thereof.
摘要翻译： 包含功能失调的fucT和xylT基因的苔藓植物和苔藓植物细胞和引入的糖基转移酶基因，用于产生糖基化蛋白质的方法，载体及其用途。
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3.发明授权Transformed plant cell expressing five mammalian proteins involved in sialylation and a protein involved in galactosylation 有权转化的植物细胞表达参与唾液酸化的5种哺乳动物蛋白质和涉及半乳糖基化的蛋白质公开(公告)号：US07741539B2
公开(公告)日：2010-06-22
申请号：US11995191
申请日：2006-07-12
CPC分类号： C12N15/8257 , C12N9/1048 , C12N9/1085 , C12N9/1205 , C12N9/90 , C12N15/8245
摘要： The present invention relates to a method for producing heterologous glycosylated proteins in non-animal eukaryotic cells such as in transformed bryophyte, yeast, ciliate or algae cells. In particular, the method relates to a method for producing glycosylated proteins comprising animal glycosylation patterns—comprising sialic acid residues—, such as pharmaceutical proteins for use in mammals, e.g. humans, in bryophyte cells such as those of Physcomitrella patens, the genetic material required therefore, such as DNA and RNA, vectors, host cells, methods of introducing genetic material there into, and uses thereof. Furthermore, the present invention relates to novel polypeptides and proteins obtained by the method according to the invention. Moreover, the present invention provides a method of producing sialic acid or CMP-sialic acid in a transformed non-mammalian eukaryotic cell, tissue or organism.
摘要翻译： 本发明涉及在非动物真核细胞如转化的苔藓植物，酵母，细胞或藻类细胞中产生异源糖基化蛋白的方法。 特别地，该方法涉及生产糖基化蛋白质的方法，其包括含有唾液酸残基的动物糖基化模式，例如用于哺乳动物的药物蛋白质，例如， 人类，例如苔藓植物细胞，例如小立碗藓的细胞，因此需要的遗传物质，例如DNA和RNA，载体，宿主细胞，将遗传物质引入其中的方法及其用途。 此外，本发明涉及通过根据本发明的方法获得的新型多肽和蛋白质。 此外，本发明提供了在转化的非哺乳动物真核细胞，组织或生物体中产生唾液酸或CMP-唾液酸的方法。
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公开(公告)号：US20080141387A1
公开(公告)日：2008-06-12
申请号：US10539725
申请日：2003-12-18
申请人： Ralf Reski , Gilbert Gorr , Eva Decker , Christian Stemmer , Otmar Lienhart , Anna Koprivova
发明人： Ralf Reski , Gilbert Gorr , Eva Decker , Christian Stemmer , Otmar Lienhart , Anna Koprivova
CPC分类号： C12N9/1051 , C12N5/04 , C12N9/1077 , C12N15/8257 , C12P21/005
摘要： Bryophyte plants and bryophyte plant cells comprising dysfunctional fucT and xylT genes and an introduced glycosyl transferase gene, methods for the production of glycosylated proteins therewith, vectors and uses thereof.
摘要翻译： 包含功能失调的fucT和xylT基因的苔藓植物和苔藓植物细胞和引入的糖基转移酶基因，用于产生糖基化蛋白质的方法，载体及其用途。
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公开(公告)号：US20090307809A1
公开(公告)日：2009-12-10
申请号：US12306928
申请日：2006-07-14
IPC分类号： G01N13/10
摘要： The scanning probe microscope has a primary control loop (7, 11, 12) for keeping the phase and/or amplitude of deflection at constant values as well as a secondary control loop (9) that e.g. keeps the frequency of the cantilever oscillation constant by applying a suitable DC voltage to the probe while, at the same time, a conservative AC excitation is applied thereto. By actively controlling the frequency with the first control loop (7, 11, 12) and subsequently controlling the DC voltage in order to keep the frequency constant, a fast system is created that allows to determine the contact potential difference or a related property of the sample (3) quickly.
摘要翻译： 扫描探针显微镜具有用于将偏转的相位和/或幅度保持在恒定值的主控制回路（7,11,12）以及例如二次回路的次级控制回路（9）。 通过向探针施加适当的直流电压来保持悬臂振荡的频率恒定，同时对其施加保守的AC激励。 通过利用第一控制回路（7,11,12）主动地控制频率并随后控制直流电压以保持频率恒定，产生快速系统，其允许确定接触电位差或相关属性 样品（3）快速。
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公开(公告)号：US20100050292A1
公开(公告)日：2010-02-25
申请号：US12443377
申请日：2007-09-28
申请人： Heike Launhardt , Christian Stemmer , Wolfgang Jost , Gilbert Gorr , Ralf Reski , Stefan Rensing
发明人： Heike Launhardt , Christian Stemmer , Wolfgang Jost , Gilbert Gorr , Ralf Reski , Stefan Rensing
CPC分类号： C12N9/1051 , C12N15/8257
摘要： The invention discloses DNA molecules encoding galactosyltransferases, recombinant host cells, tissues or organisms comprising dysfunctional galactosyltransferase gene(s), recombinant host cells, tissues or organisms comprising an introduced functional galactosyltransferase gene, methods for the production of proteins therewith, methods for the production of galactosyltransferase and vectors and uses thereof.
摘要翻译： 本发明公开了编码半乳糖基转移酶，重组宿主细胞，包含功能失活的半乳糖基转移酶基因的组织或生物的DNA分子，重组宿主细胞，包含引入的功能性半乳糖基转移酶基因的组织或生物，用于生产蛋白质的方法， 半乳糖基转移酶和载体及其用途。
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公开(公告)号：US20080201804A1
公开(公告)日：2008-08-21
申请号：US11995191
申请日：2006-07-12
IPC分类号： A01H5/00 , C12N5/10 , C12P21/00 , C12N15/82 , C12N1/21 , C12P19/26 , C12N1/19 , C12N1/13 , C07H21/04
CPC分类号： C12N15/8257 , C12N9/1048 , C12N9/1085 , C12N9/1205 , C12N9/90 , C12N15/8245
摘要： The present invention relates to a method for producing heterologous glycosylated proteins in non-animal eukaryotic cells such as in transformed bryophyte, yeast, ciliate or algae cells. In particular, the method relates to a method for producing glycosylated proteins comprising animal glycosylation patterns—comprising sialic acid residues—, such as pharmaceutical proteins for use in mammals, e.g. humans, in bryophyte cells such as those of Physcomitrella patens, the genetic material required therefore, such as DNA and RNA, vectors, host cells, methods of introducing genetic material there into, and uses thereof. Furthermore, the present invention relates to novel polypeptides and proteins obtained by the method according to the invention. Moreover, the present invention provides a method of producing sialic acid or CMP-sialic acid in a transformed non-mammalian eukaryotic cell, tissue or organism.
摘要翻译： 本发明涉及在非动物真核细胞如转化的苔藓植物，酵母，细胞或藻类细胞中产生异源糖基化蛋白的方法。 特别地，该方法涉及生产糖基化蛋白质的方法，其包括含有唾液酸残基的动物糖基化模式，例如用于哺乳动物的药物蛋白质，例如， 人类，例如苔藓植物细胞，例如小立碗藓的细胞，因此需要的遗传物质，例如DNA和RNA，载体，宿主细胞，将遗传物质引入其中的方法及其用途。 此外，本发明涉及通过根据本发明的方法获得的新型多肽和蛋白质。 此外，本发明提供了在转化的非哺乳动物真核细胞，组织或生物体中产生唾液酸或CMP-唾液酸的方法。
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