公开(公告)号：US06835552B2

公开(公告)日：2004-12-28

申请号：US09737542

申请日：2000-12-14

申请人： Robin R. Miles ,  Kodumudi S. Venkateswaran ,  Christopher K. Fuller

发明人： Robin R. Miles ,  Kodumudi S. Venkateswaran ,  Christopher K. Fuller

IPC分类号： G01N3353

CPC分类号： G01N33/5438 ,  G01N27/3276 ,  Y10S435/817 ,  Y10S436/806 ,  Y10S436/823

摘要： The use of impedance measurements to detect the presence of pathogens attached to antibody-coated beads. In a fluidic device antibodies are immobilized on a surface of a patterned interdigitated electrode. Pathogens in a sample fluid streaming past the electrode attach to the immobilized antibodies, which produces a change in impedance between two adjacent electrodes, which impedance change is measured and used to detect the presence of a pathogen. To amplify the signal, beads coated with antibodies are introduced and the beads would stick to the pathogen causing a greater change in impedance between the two adjacent electrodes.

摘要翻译： 使用阻抗测量来检测与抗体包被的珠粒相连的病原体的存在。 在流体装置中，将抗体固定在图案化交错电极的表面上。 流经电极的样品流体中的病原体附着于固定的抗体，其产生两个相邻电极之间的阻抗变化，该阻抗变化被测量并用于检测病原体的存在。 为了扩增信号，引入用抗体包被的珠粒，并且珠粒将粘附到病原体上，导致两个相邻电极之间的阻抗变化更大。

公开(公告)号：US06846639B2

公开(公告)日：2005-01-25

申请号：US09993870

申请日：2001-11-15

申请人： Robin R. Miles ,  Kodumudi S. Venkateswaran ,  Christopher K. Fuller

发明人： Robin R. Miles ,  Kodumudi S. Venkateswaran ,  Christopher K. Fuller

IPC分类号： G01N27/02 ,  G01N33/543 ,  G01N33/53

CPC分类号： G01N33/5438 ,  G01N27/3276 ,  Y10S435/817 ,  Y10S436/806 ,  Y10S436/823

摘要： The use of impedance measurements to detect the presence of pathogens attached to antibody-coated beads. In a fluidic device antibodies are immobilized on a surface of a patterned interdigitated electrode. Pathogens in a sample fluid streaming past the electrode attach to the immobilized antibodies, which produces a change in impedance between two adjacent electrodes, which impedance change is measured and used to detect the presence of a pathogen. To amplify the signal, beads coated with antibodies are introduced and the beads would stick to the pathogen causing a greater change in impedance between the two adjacent electrodes.

摘要翻译： 使用阻抗测量来检测与抗体包被的珠粒相连的病原体的存在。 在流体装置中，将抗体固定在图案化交错电极的表面上。 流经电极的样品流体中的病原体附着于固定的抗体，其产生两个相邻电极之间的阻抗变化，该阻抗变化被测量并用于检测病原体的存在。 为了扩增信号，引入用抗体包被的珠粒，并且珠粒将粘附到病原体上，导致两个相邻电极之间的阻抗变化更大。

公开(公告)号：US06787018B1

公开(公告)日：2004-09-07

申请号：US09733857

申请日：2000-12-08

申请人： Robin R. Miles ,  Kerry A. Bettencourt ,  Christopher K. Fuller

发明人： Robin R. Miles ,  Kerry A. Bettencourt ,  Christopher K. Fuller

IPC分类号： G01N24447

CPC分类号： B01L3/502761 ,  B01L2200/0668 ,  B01L2400/0418 ,  B01L2400/0424 ,  B03C5/026 ,  G01N15/0656 ,  G01N2001/4038

摘要： The use of dielectrophoresis to collect particles under the conditions of electrokinetically-driven flow. Dielectrophortic concentration of particles under electrokinetic flow is accomplished by interdigitated electrodes patterned on an inner surface of a microfluid channel, a DC voltage is applied across the ends to the channel, and an AC voltage is applied across the electrodes, and particles swept down the channel electrokinetically are trapped within the field established by the electrodes. The particles can be released when the voltage to the electrodes is released.

摘要翻译： 在电动驱动流动条件下使用介电电泳收集颗粒。 在电动流动下颗粒的电极间主动脉浓度通过图案化在微流体通道的内表面上的交错电极实现，直流电压跨越通道施加，并且在电极之间施加AC电压，并且将颗粒扫过通道 电动地被捕获在由电极建立的场内。 当电极的电压被释放时，可以释放颗粒。

公开(公告)号：US06576459B2

公开(公告)日：2003-06-10

申请号：US09815624

申请日：2001-03-23

申请人： Robin R. Miles ,  Amy W. Wang ,  Christopher K. Fuller ,  Asuncion V. Lemoff ,  Kerry A. Bettencourt ,  June Yu

发明人： Robin R. Miles ,  Amy W. Wang ,  Christopher K. Fuller ,  Asuncion V. Lemoff ,  Kerry A. Bettencourt ,  June Yu

IPC分类号： C12M136

CPC分类号： B01L3/5027 ,  B01F13/0059 ,  B01F13/0076 ,  B01L7/52 ,  B01L2200/10 ,  B01L2400/0424 ,  B01L2400/043 ,  G01N1/4077 ,  G01N15/0656 ,  G01N2015/0088 ,  Y10S435/81 ,  Y10S435/962

摘要： A sample preparation and analysis device which incorporates both immunoassays and PCR assays in one compact, field-portable microchip. The device provides new capabilities in fluid and particle control which allows the building of a fluidic chip with no moving parts, thus decreasing fabrication cost and increasing the robustness of the device. The device can operate in a true continuous (not batch) mode. The device incorporates magnetohydrodynamic (MHD) pumps to move the fluid through the system, acoustic mixing and fractionation, dielectropheretic (DEP) sample concentration and purification, and on-chip optical detection capabilities.

公开(公告)号：US06437551B1

公开(公告)日：2002-08-20

申请号：US09704355

申请日：2000-11-01

申请人： Peter Krulevitch ,  Harold D. Ackler ,  Frederick Becker ,  Bernhard E. Boser ,  Adam B. Eldredge ,  Christopher K. Fuller ,  Peter R. C. Gascoyne ,  Julie K. Hamilton ,  Stefan P. Swierkowski ,  Xiao-Bo Wang

发明人： Peter Krulevitch ,  Harold D. Ackler ,  Frederick Becker ,  Bernhard E. Boser ,  Adam B. Eldredge ,  Christopher K. Fuller ,  Peter R. C. Gascoyne ,  Julie K. Hamilton ,  Stefan P. Swierkowski ,  Xiao-Bo Wang

IPC分类号： G01N2700

CPC分类号： G01N33/5438 ,  B01L3/5027 ,  G01N27/026 ,  G01N2015/129

摘要： A microfabricated instrument for detecting and identifying cells and other particles based on alternating current (AC) impedance measurements. The microfabricated AC impedance sensor includes two critical elements: 1) a microfluidic chip, preferably of glass substrates, having at least one microchannel therein and with electrodes patterned on both substrates, and 2) electrical circuits that connect to the electrodes on the microfluidic chip and detect signals associated with particles traveling down the microchannels. These circuits enable multiple AC impedance measurements of individual particles at high throughput rates with sufficient resolution to identify different particle and cell types as appropriate for environmental detection and clinical diagnostic applications.

摘要翻译： 一种用于基于交流（AC）阻抗测量来检测和识别电池和其他颗粒的微加工仪器。 微制造的AC阻抗传感器包括两个关键元件：1）优选玻璃基板的微流体芯片，其中至少有一个微通道，并且在两个基板上图案化电极，以及2）连接到微流体芯片上的电极的电路，以及 检测与微通道下游的颗粒相关联的信号。 这些电路可以以高吞吐量进行多个交流阻抗测量，具有足够的分辨率，以识别适用于环境检测和临床诊断应用的不同粒子和细胞类型。