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公开(公告)号：US07148033B2
公开(公告)日：2006-12-12
申请号：US10713113
申请日：2003-11-17
摘要： An improved method for detection of total coliforms and E. coli comprising a broth containing an ingredient that will suppress growth of non-coliform gram negative bacteria, and at least one chromogen or fluorogen has been used effectively and is cost effective. Preferred methods include use of filter and/or plates containing the growth-promoting ingredients and the indicators.
摘要翻译： 检测总大肠菌群和大肠杆菌的改进方法包括含有抑制非大肠杆菌革兰氏阴性细菌生长的成分的肉汤，以及至少一种色原或荧光素，并且是有成本效益的。 优选的方法包括使用含有生长促进成分和指示剂的过滤器和/或板。
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公开(公告)号：US06670145B2
公开(公告)日：2003-12-30
申请号：US09924950
申请日：2001-08-09
IPC分类号： C12Q104
摘要： An improved method for detection of total coliforms and E. coli comprising a broth containing an ingredient that will encourage growth and repair of injured coliforms, buffers to maintain a pH in the range of 6.5-8, at least one agent that suppresses growth of gram positive cocci and spore-forming organisms, at least one active agent that will suppress growth of non-coliform gram negative bacteria, and at least one chromogen or fluorogen has been used effectively and is cost effective. In the preferred embodiment, both a fluorogen and chromogen were used. Preferred methods include use of filter and/or plates containing the growth-promoting ingredients and the indicators.
摘要翻译： 一种用于检测总大肠杆菌和大肠杆菌的改进方法，其包含含有促进受损大肠菌群生长和修复的成分的培养液，将pH保持在6.5-8范围内的缓冲剂，至少一种抑制克生长的试剂 阳性球菌和孢子形成生物体，至少一种抑制非大肠杆菌革兰氏阴性细菌生长的活性剂和至少一种色原或荧光素被有效地使用并且是成本有效的。 在优选的实施方案中，使用氟和色原。 优选的方法包括使用含有生长促进成分和指示剂的过滤器和/或板。
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3.发明授权Membrane filter agar medium containing two enzyme substrates used for the simultaneous detection of total coliforms and E. coli. 失效含有两个酶底物的膜过滤器琼脂培养基，用于同时检测总大肠杆菌和大肠杆菌。
公开(公告)号：US6063590A
公开(公告)日：2000-05-16
申请号：US117342
申请日：1993-09-07
摘要： An improved method for detection of total coliforms and E. coli comprising placing the target sample in a broth containing an ingredient that will encourage growth and repair of injured coliforms, at least one agent that suppresses growth of gram positive cocci and spore-forming organisms, at least one active agent that will suppress growth of non-coliform gram negative bacteria, and at least one chromogen or fluorogen has been used effectively and is cost effective. In the preferred embodiment, both a fluorogen and chromogen were used. Preferred methods include use of filter and/or plates containing the growth-promoting ingredients and the indicators.
摘要翻译： 一种用于检测总大肠菌群和大肠杆菌的改进方法，包括将目标样品置于含有促进受损大肠菌群生长和修复的成分的培养液中，至少一种抑制革兰氏阳性球菌和孢子形成生物生长的试剂， 至少一种抑制非大肠杆菌革兰氏阴性细菌生长的活性剂，至少有一种色原体或荧光素被有效利用并且是成本有效的。 在优选的实施方案中，使用氟和色原。 优选的方法包括使用含有生长促进成分和指示剂的过滤器和/或板。
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4.发明授权Membrane filter agar medium for simultaneous detection of total coliforms and E. coli 有权用于同时检测总大肠杆菌和大肠杆菌的膜过滤器琼脂培养基公开(公告)号：US06306621B1
公开(公告)日：2001-10-23
申请号：US09369173
申请日：1999-08-05
IPC分类号： C12Q110
摘要： An improved method for detection of total coliforms and E. coli comprising a broth containing an ingredient that will encourage growth and repair of injured coliforms, buffers to maintain a pH in the range of 6.5-8, at least one agent that suppresses growth of gram positive cocci and spore-forming organisms, at least one active agent that will suppress growth of non-coliform gram negative bacteria, and at least one chromogen or fluorogen has been used effectively and is cost effective. In the preferred embodiment, both a fluorogen and chromogen were used. Preferred methods include use of filter and/or plates containing the growth-promoting ingredients and the indicators.
摘要翻译： 一种用于检测总大肠杆菌和大肠杆菌的改进方法，其包含含有促进受损大肠菌群生长和修复的成分的培养液，将pH保持在6.5-8范围内的缓冲剂，至少一种抑制克生长的试剂 阳性球菌和孢子形成生物体，至少一种抑制非大肠杆菌革兰氏阴性细菌生长的活性剂和至少一种色原或荧光素被有效地使用并且是成本有效的。 在优选的实施方案中，使用氟和色原。 优选的方法包括使用含有生长促进成分和指示剂的过滤器和/或板。
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