公开(公告)号：US08632789B2

公开(公告)日：2014-01-21

申请号：US13286847

申请日：2011-11-01

申请人： Mathew Maye ,  James Dabrowiak ,  Colleen Alexander

发明人： Mathew Maye ,  James Dabrowiak ,  Colleen Alexander

IPC分类号： A61K9/14 ,  A61P35/00 ,  B82B1/00

CPC分类号： A61K31/7042 ,  A61K47/6923 ,  Y10T428/2982

摘要： System and method for loading the front line anticancer drug, doxorubicin (DOX) onto DNA-capped gold nanoparticles whose duplex DNA has been designed for specific DOX intercalation. Since each AuNP contains about 108 high affinity drug sites, this design allows for a high local DOX concentration on the particle. Drug binding was confirmed by monitoring the increase in DNA melting temperature, the shift in the plasmon resonance maximum, and the increase in the NP hydrodynamic radius as a function of [DOX]/[DNA] ratio. The feasibility of the nanoparticles as a drug delivery system was demonstrated by showing that particle-bound DOX could be transferred to a target DNA.

摘要翻译： 将前线抗癌药物多柔比星（DOX）加载到DNA封端的金纳米颗粒上的系统和方法，其双链DNA已被设计用于特定的DOX插层。 由于每个AuNP含有约108个高亲和力的药物部位，因此该设计允许颗粒上的高的局部DOX浓度。 通过监测DNA熔解温度的升高，等离子体共振最大值的移动和作为[DOX] / [DNA]比率的函数的NP流体动力学半径的增加来证实药物结合。 通过显示粒子结合的DOX可以转移到靶DNA来证明纳米颗粒作为药物递送系统的可行性。

公开(公告)号：US20120141550A1

公开(公告)日：2012-06-07

申请号：US13286847

申请日：2011-11-01

申请人： Mathew Maye ,  James Dabrowiak ,  Colleen Alexander

发明人： Mathew Maye ,  James Dabrowiak ,  Colleen Alexander

IPC分类号： A61K9/14 ,  C07H1/00 ,  A61K31/7135 ,  B32B5/16 ,  C07H21/00 ,  A61P35/00 ,  B82B3/00 ,  B82B1/00 ,  B82Y5/00

CPC分类号： A61K31/7042 ,  A61K47/6923 ,  Y10T428/2982

摘要： System and method for loading the front line anticancer drug, doxorubicin (DOX) onto DNA-capped gold nanoparticles whose duplex DNA has been designed for specific DOX intercalation. Since each AuNP contains about 108 high affinity drug sites, this design allows for a high local DOX concentration on the particle. Drug binding was confirmed by monitoring the increase in DNA melting temperature, the shift in the plasmon resonance maximum, and the increase in the NP hydrodynamic radius as a function of [DOX]/[DNA] ratio. The feasibility of the nanoparticles as a drug delivery system was demonstrated by showing that particle-bound DOX could be transferred to a target DNA.

摘要翻译： 将前线抗癌药物多柔比星（DOX）加载到DNA封端的金纳米颗粒上的系统和方法，其双链DNA已被设计用于特定的DOX插层。 由于每个AuNP含有约108个高亲和力的药物部位，因此该设计允许颗粒上的高的局部DOX浓度。 通过监测DNA熔解温度的升高，等离子体共振最大值的移动和作为[DOX] / [DNA]比率的函数的NP流体动力学半径的增加来证实药物结合。 通过显示粒子结合的DOX可以转移到靶DNA来证明纳米颗粒作为药物递送系统的可行性。