公开(公告)号：US07283228B2

公开(公告)日：2007-10-16

申请号：US10821231

申请日：2004-04-08

申请人： Dongmao Zhang ,  Dor Ben-Amotz ,  Yong Xie ,  Vincent J. Davisson ,  Melissa Mrozek ,  Corasi Ortiz

发明人： Dongmao Zhang ,  Dor Ben-Amotz ,  Yong Xie ,  Vincent J. Davisson ,  Melissa Mrozek ,  Corasi Ortiz

IPC分类号： G01J3/44 ,  G01N21/65 ,  G01N1/00

CPC分类号： G01N21/3563 ,  B82Y30/00 ,  G01N21/658 ,  G01N2001/4027 ,  G01N2021/3595

摘要： Micro-droplets of liquid confining organic molecules of interest are dried on selected planar solvo-phobic substrates under conditions facilitating segregated precipitation of the larger, less soluable analytes toward edge portions of the deposit. Micro-spectrometer imaging using white light and FTIR false color can identify points of interest, and the same optics generally directed perpendicularly to the substrates selectively captures normal Raman spectra from selected points in the deposit. The spectra are manipulated using various data techniques to extract reliable information concerning analytes present at pico-Molar levels. The selected spots can also be subjected to FTIR spectroscopy followed by MALDI mass spectroscopy to obtain a variety of information from the identical specimen.

摘要翻译： 限制有目标有机分子的液滴微粒在有选择的平面溶剂基底上干燥，条件是使较大的较少溶解性分析物朝向沉积物的边缘部分分离析出。 使用白光和FTIR假色的微光谱仪成像可以识别感兴趣的点，并且通常垂直于衬底定向的相同光学器件选择性地从沉积物中的选定点捕获正常的拉曼光谱。 使用各种数据技术来操作光谱，以提取关于以微微摩尔浓度存在的分析物的可靠信息。 所选择的点也可以进行FTIR光谱，然后进行MALDI质谱，从相同的样品获得各种信息。

公开(公告)号：US20050275837A1

公开(公告)日：2005-12-15

申请号：US10821231

申请日：2004-04-08

申请人： Dongmao Zhang ,  Dor Ben-Amotz ,  Yong Xie ,  Vincent Davisson ,  Melissa Mrozek ,  Corasi Ortiz

发明人： Dongmao Zhang ,  Dor Ben-Amotz ,  Yong Xie ,  Vincent Davisson ,  Melissa Mrozek ,  Corasi Ortiz

IPC分类号： G01N21/65 ,  G01J3/44

CPC分类号： G01N21/3563 ,  B82Y30/00 ,  G01N21/658 ,  G01N2001/4027 ,  G01N2021/3595

摘要： Micro-droplets of liquid confining organic molecules of interest are dried on selected planar solvo-phobic substrates under conditions facilitating segregated precipitation of the larger, less soluable analytes toward edge portions of the deposit. Micro-spectrometer imaging using white light and FTIR false color can identify points of interest, and the same optics generally directed perpendicularly to the substrates selectively captures normal Raman spectra from selected points in the deposit. The spectra are manipulated using various data techniques to extract reliable information concerning analytes present at pico-Molar levels. The selected spots can also be subjected to FTIR spectroscopy followed by MALDI mass spectroscopy to obtain a variety of information from the identical specimen.

摘要翻译： 限制有目标有机分子的液滴微粒在有选择的平面溶剂基底上干燥，条件是使较大的较少溶解性分析物朝向沉积物的边缘部分分离析出。 使用白光和FTIR假色的微光谱仪成像可以识别感兴趣的点，并且通常垂直于衬底定向的相同光学器件选择性地从沉积物中的选定点捕获正常的拉曼光谱。 使用各种数据技术来操作光谱，以提取关于以微微摩尔浓度存在的分析物的可靠信息。 所选择的点也可以进行FTIR光谱，然后进行MALDI质谱，从相同的样品获得各种信息。