公开(公告)号：US20130165334A1

公开(公告)日：2013-06-27

申请号：US13582558

申请日：2011-03-04

申请人： Andrew A. Bieberich ,  Lova N. Rakotomalala ,  Vincent J. Davisson ,  Joseph P. Robinson

发明人： Andrew A. Bieberich ,  Lova N. Rakotomalala ,  Vincent J. Davisson ,  Joseph P. Robinson

IPC分类号： C12Q1/70

CPC分类号： C12Q1/708 ,  C12Q1/6804 ,  C12Q2600/16 ,  C12Q2565/125 ,  C12Q2537/143 ,  C12Q2531/113

摘要： A two part assay is disclosed that enables collection of both protein biomarker phenotype and specific HPV genotype data from within a clinically derived population of cervical epithelial cells. Presence of multiple transformation-associated protein biomarkers acts as a gating criterion for cell sorting, followed by application of a PCR protocol sensitive enough to detect and identify individual HPV types from within the cells captured during sorting. The workflow has been optimized to work with cells conventionally fixed in PreservCyt (Cytyc), and it can be performed on residual cells remaining in a stored sample after a Pap test has been performed.

摘要翻译： 公开了两部分测定法，其能够从临床衍生的宫颈上皮细胞群体中收集蛋白质生物标志物表型和特异性HPV基因型数据。 多重转化相关蛋白生物标志物的存在作为细胞分选的门控标准，随后应用足够敏感的PCR方案来检测和鉴定分选期间捕获的细胞内的各种HPV类型。 已经优化了工作流程以与常规固定在PreservCyt（Cytyc）中的细胞一起工作，并且可以在执行Pap测试之后对残留在存储的样品中的残留细胞进行。

公开(公告)号：US07283228B2

公开(公告)日：2007-10-16

申请号：US10821231

申请日：2004-04-08

申请人： Dongmao Zhang ,  Dor Ben-Amotz ,  Yong Xie ,  Vincent J. Davisson ,  Melissa Mrozek ,  Corasi Ortiz

发明人： Dongmao Zhang ,  Dor Ben-Amotz ,  Yong Xie ,  Vincent J. Davisson ,  Melissa Mrozek ,  Corasi Ortiz

IPC分类号： G01J3/44 ,  G01N21/65 ,  G01N1/00

CPC分类号： G01N21/3563 ,  B82Y30/00 ,  G01N21/658 ,  G01N2001/4027 ,  G01N2021/3595

摘要： Micro-droplets of liquid confining organic molecules of interest are dried on selected planar solvo-phobic substrates under conditions facilitating segregated precipitation of the larger, less soluable analytes toward edge portions of the deposit. Micro-spectrometer imaging using white light and FTIR false color can identify points of interest, and the same optics generally directed perpendicularly to the substrates selectively captures normal Raman spectra from selected points in the deposit. The spectra are manipulated using various data techniques to extract reliable information concerning analytes present at pico-Molar levels. The selected spots can also be subjected to FTIR spectroscopy followed by MALDI mass spectroscopy to obtain a variety of information from the identical specimen.

摘要翻译： 限制有目标有机分子的液滴微粒在有选择的平面溶剂基底上干燥，条件是使较大的较少溶解性分析物朝向沉积物的边缘部分分离析出。 使用白光和FTIR假色的微光谱仪成像可以识别感兴趣的点，并且通常垂直于衬底定向的相同光学器件选择性地从沉积物中的选定点捕获正常的拉曼光谱。 使用各种数据技术来操作光谱，以提取关于以微微摩尔浓度存在的分析物的可靠信息。 所选择的点也可以进行FTIR光谱，然后进行MALDI质谱，从相同的样品获得各种信息。

公开(公告)号：US20100291599A1

公开(公告)日：2010-11-18

申请号：US12467440

申请日：2009-05-18

申请人： Thomas J. Tague, JR. ,  William Kopaciewicz ,  Vincent J. Davisson ,  Elena Chernokalskaya ,  Timothy S. Rider ,  Cruz A.D. Hinojos ,  Jun Zhao

发明人： Thomas J. Tague, JR. ,  William Kopaciewicz ,  Vincent J. Davisson ,  Elena Chernokalskaya ,  Timothy S. Rider ,  Cruz A.D. Hinojos ,  Jun Zhao

IPC分类号： G01N33/53 ,  G01N21/65 ,  G01J3/44

CPC分类号： G01N21/658 ,  G01J3/44 ,  G01N21/65 ,  G01N33/54373

摘要： Raman spectra of protein immunoblots or enzyme linked immunosorbant assay procedures are acquired with a scanning Raman spectrometer. The sensitivity of the measurement is increased by conjugating secondary antibodies used in the Western blot and ELISA methods to surface enhanced Raman Scattering (SERS) labels. The resulting blot or well plate is analyzed with a Raman system that has forms a pixel map of the sample. More specifically, the Raman system generates an effectively line-shaped illumination pattern and scans the sample in the direction perpendicular to the line while the signal is accumulating on the detector. Each pixel is therefore a rectangle defined by the length of the illumination and the distance traveled by the sample within the duration of signal accumulation on the detector. The pixels are sequentially acquired to generate a map of the sample.

摘要翻译： 使用扫描拉曼光谱仪获得蛋白免疫印迹或酶联免疫吸附测定程序的拉曼光谱。 通过将Western印迹和ELISA方法中使用的二次抗体与表面增强的拉曼散射（SERS）标记缀合，来增加测量的灵敏度。 用形成样品的像素图的拉曼系统分析所得到的印迹或孔板。 更具体地，拉曼系统产生有效的线状照明图案，并且在信号积聚在检测器上的同时沿垂直于线的方向扫描样品。 因此，每个像素是由照明长度和样本在检测器上的信号累积持续时间内行进的距离所定义的矩形。 依次获取像素以生成样本的图。