公开(公告)号：US10851141B2

公开(公告)日：2020-12-01

申请号：US16311301

申请日：2017-06-19

Applicant: DKK-TOA Corporation

Inventor： Kenichi Noda ,  Satoshi Yawata ,  Ai Shimomura

IPC: C12N15/10 ,  C12N15/09 ,  C12P21/02 ,  C07H21/04 ,  C07K14/435 ,  C12N9/02 ,  C12N15/63

Abstract: The present invention provides a mutant beetle luciferase and the like, having mutation in which the amino acid corresponding to valine at position 288 in the amino acid sequence of wild-type Photinus pyralis luciferase is isoleucine, leucine or phenylalanine, mutation in which the amino acid corresponding to leucine at position 376 in the aforementioned sequence is proline, mutation in which the amino acid corresponding to glutamic acid at position 455 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, or mutation in which the amino acid corresponding to glutamic acid at position 488 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, in the amino acid sequence encoding a wild-type beetle luciferase, and characterized in that a luminescence intensity due to a luciferin-luciferase luminescence reaction in a 0.9% by mass NaCl solution is 50% or more of that in a NaCl-free solution.

公开(公告)号：US20190352350A1

公开(公告)日：2019-11-21

申请号：US16311301

申请日：2017-06-19

Applicant: DKK-TOA Corporation

Inventor： Kenichi Noda ,  Satoshi Yawata ,  Ai Shimomura

IPC: C07K14/435 ,  C12P21/02 ,  C12N9/02 ,  C12N15/63

Abstract: The present invention provides a mutant beetle luciferase and the like, having mutation in which the amino acid corresponding to valine at position 288 in the amino acid sequence of wild-type Photinus pyralis luciferase is isoleucine, leucine or phenylalanine, mutation in which the amino acid corresponding to leucine at position 376 in the aforementioned sequence is proline, mutation in which the amino acid corresponding to glutamic acid at position 455 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, or mutation in which the amino acid corresponding to glutamic acid at position 488 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, in the amino acid sequence encoding a wild-type beetle luciferase, and characterized in that a luminescence intensity due to a luciferin-luciferase luminescence reaction in a 0.9% by mass NaCl solution is 50% or more of that in a NaCl-free solution.

公开(公告)号：US20160178592A1

公开(公告)日：2016-06-23

申请号：US14900396

申请日：2014-05-12

Applicant: KURITA WATER INDUSTRIES LTD. ,  DKK-TOA CORPORATION

Inventor： Shintarou MORI ,  Yukimasa SHIMURA ,  Tetsuya SATO ,  Satoshi TAKIZAWA

IPC: G01N33/18 ,  G01N21/25 ,  G01N21/59

CPC classification number: G01N33/18 ,  G01N21/03 ,  G01N21/255 ,  G01N21/59 ,  G01N2021/0325 ,  G01N2021/0367

Abstract: Provided is a device for measuring the concentration of a dissolved component, whereby the concentration of a plurality of types of dissolved components in water can be measured easily and with good precision. A device for measuring the concentration of a dissolved component has: a cell assembly 3 in which a plurality of cells 1 are connected by a joint member 2 and integrated, and a measurement reagent is accommodated in each of the cells 1; and a measuring main body 5 having a measurement unit (light-emitting element 8 and light-receiving element 9) for measuring transmittance and absorbance, the cells 1 of the cell assembly 3 being inserted into the measurement unit. A cap 4 is detachably mounted to each cell 1.

Abstract translation: 提供了用于测量溶解组分浓度的装置，由此可以容易且精确地测量多种溶解在水中的溶解组分的浓度。 用于测量溶解成分的浓度的装置具有：多个电池单元1通过接头构件2连接并整合的电池组件3，并且测量试剂容纳在每个电池单元1中; 以及测量主体5，其具有用于测量透射率和吸光度的测量单元（发光元件8和光接收元件9），单元组件3的单元1插入到测量单元中。 帽4可拆卸地安装到每个电池单元1。

公开(公告)号：US20150225768A1

公开(公告)日：2015-08-13

申请号：US14429784

申请日：2013-09-19

Applicant: DKK-TOA CORPORATION ,  HIROSHIMA UNIVERSITY

Inventor： Satoshi Yawata ,  Hiromitsu Hachiya ,  Akio Kuroda ,  Kenichi Noda

IPC: C12Q1/66 ,  C12Q1/00

CPC classification number: C12Q1/66 ,  C12Q1/008 ,  G01N21/76 ,  G01N33/579 ,  G01N2400/24 ,  G01N2400/50

Abstract: A quantification method includes a calibration curve preparing step to measure a standard solution, which has been prepared by adding sodium ions so that a sodium ion content of the standard solution is equaled to a sodium ion content of a sample to be measured with a method employing a reaction that activates a limulus reagent and/or a biochemical luminescent reaction caused by ATP, luciferin, and luciferase, and to prepare a calibration curve that represents a relation between a measurement value and an amount of a component to be measured; a sample measuring step to measure the sample to be measured with a method being the same as that used in the calibration curve preparing step; and a quantifying step to find, by using the calibration curve, an amount of the component to be measured in the sample to be measured from a measurement value in the sample measuring step.

Abstract translation: 定量方法包括：通过添加钠离子制备标准溶液的标准溶液的校准曲线制备步骤，使标准溶液的钠离子含量与待测样品的钠离子含量相等， 激活鲎试剂和/或由ATP，荧光素和荧光素酶引起的生化发光反应的反应，并制备表示测量值与待测组分量之间的关系的校准曲线; 样品测量步骤，用与校准曲线制备步骤中使用的方法相同的方法测量待测样品; 以及量化步骤，通过使用校准曲线，从样品测量步骤中的测量值来求出待测样品中待测量的成分的量。

公开(公告)号：USD728399S1

公开(公告)日：2015-05-05

申请号：US29478601

申请日：2014-01-07

Applicant: DKK-TOA Corporation ,  Kurita Water Industries Ltd.

Designer： Satoshi Takizawa ,  Tetsuya Sato ,  Shuji Sugawara ,  Yuko Konno ,  Shintarou Mori ,  Yukimasa Shimura