公开(公告)号：US20100267093A1

公开(公告)日：2010-10-21

申请号：US12760461

申请日：2010-04-14

申请人： Donald L. Court ,  Daiguan Yu ,  E-Chiang Lee ,  Hilary M. Ellis ,  Nancy A. Jenkins ,  Pentao Liu ,  Neal G. Copeland

发明人： Donald L. Court ,  Daiguan Yu ,  E-Chiang Lee ,  Hilary M. Ellis ,  Nancy A. Jenkins ,  Pentao Liu ,  Neal G. Copeland

IPC分类号： C12N15/87

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  C12N15/66 ,  C12N15/8213 ,  C12N15/902 ,  C12N2800/204 ,  C12N2800/30 ,  C12N2830/002 ,  C12N2830/55 ,  C12N2830/60 ,  C12N2840/203

摘要： Disclosed herein are methods for generating recombinant DNA molecules in cells using homologous recombination mediated by recombinases and similar proteins. The methods promote high efficiency homologous recombination in bacterial cells, and in eukaryotic cells such as mammalian cells. The methods are useful for cloning, the generation of transgenic and knockout animals, and gene replacement. The methods are also useful for subcloning large DNA fragments without the need for restriction enzymes. The methods are also useful for repairing single or multiple base mutations to wild type or creating specific mutations in the genome. Also disclosed are bacterial strains and vectors which are useful for high-efficiency homologous recombination.

摘要翻译： 本文公开了使用由重组酶和类似蛋白质介导的同源重组在细胞中产生重组DNA分子的方法。 这些方法促进细菌细胞和真核细胞如哺乳动物细胞中的高效同源重组。 该方法可用于克隆，转基因和敲除动物的产生以及基因替换。 该方法也可用于亚克隆大的DNA片段而不需要限制酶。 该方法对修复单基因或多基因突变至野生型或在基因组中产生特异性突变也是有用的。 还公开了可用于高效同源重组的细菌菌株和载体。

公开(公告)号：US07144734B2

公开(公告)日：2006-12-05

申请号：US10366044

申请日：2003-02-12

申请人： Donald L. Court ,  Daiguan Yu ,  E-Chiang Lee ,  Hilary M. Ellis ,  Nancy A. Jenkins ,  Neal G. Copeland

发明人： Donald L. Court ,  Daiguan Yu ,  E-Chiang Lee ,  Hilary M. Ellis ,  Nancy A. Jenkins ,  Neal G. Copeland

IPC分类号： C12N15/00 ,  C12N15/87 ,  C12N15/74 ,  C12N15/79 ,  C12N15/80 ,  C12N15/82 ,  C12N1/16 ,  C12N1/20

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  C12N15/66 ,  C12N15/8213 ,  C12N15/902 ,  C12N2800/204 ,  C12N2800/30 ,  C12N2830/002 ,  C12N2830/55 ,  C12N2830/60 ,  C12N2840/203

摘要： Disclosed herein are methods for generating recombinant DNA molecules in cells using homologous recombination mediated by recombinases and similar proteins. The methods promote high efficiency homologous recombination in bacterial cells, and in eukaryotic cells such as mammalian cells. The methods are useful for cloning, the generation of transgenic and knockout animals, and gene replacement. The methods are also useful for subcloning large DNA fragments without the need for restriction enzymes. The methods are also useful for repairing single or multiple base mutations to wild type or creating specific mutations in the genome. Also disclosed are bacterial strains and vectors which are useful for high-efficiency homologous recombination.