公开(公告)号：US07585461B2

公开(公告)日：2009-09-08

申请号：US10766614

申请日：2004-01-28

申请人： Lawrence A. Shimp ,  Sheldon Dean ,  Daniel Martins

发明人： Lawrence A. Shimp ,  Sheldon Dean ,  Daniel Martins

IPC分类号： A61L2/18

CPC分类号： A61L2/0088 ,  A61F2/28 ,  A61F2002/2835 ,  A61F2002/302 ,  A61F2002/4646 ,  A61F2002/4649 ,  A61F2230/0065 ,  A61L2/0011

摘要： Bone such as cortical or cancellous bone or connective tissue are centrifuged as a solution is applied to the tissue in a continuous process. The centrifuge creates forces, which cause the flowing solution to penetrate substantially all of the cavities of the tissue. The solution, which may be alcohol, a detergent, an oxidizer, or a surfactant, with or without water, flushes the cavities of the tissue and removes and inactivates viral and bacterial contaminants. In a second embodiment, bone or connective tissue is centrifuged in a batch process to remove contaminants from cavities in the tissue by first spinning the tissue dry and then submerging the tissue in a viral/bacteria cleansing solution and centrifuging the submerged tissue and solution at a sufficiently high speed and radius from the spin axis to create substantially high G forces on the tissue and solution to force the solution into and through the tissue cavities. The solution with the flushed contaminants is then removed from the vicinity of the tissue. In a further embodiment, an enzyme is centrifuged with the tissue to digest lipids and proteins which are then removed with a flushing solution which may also inactivate viral and bacterial contaminants. In yet a further embodiment, the centrifuge can be used to infuse biologically and/or structurally useful materials into the tissue. Further, the lipids and proteins may be removed by a chemically active agent such as oxo anions.

摘要翻译： 将骨骼如皮层或松质骨或结缔组织离心，将溶液以连续方式施用于组织。 离心机产生力，这使得流动的溶液基本上穿透组织的所有空腔。 溶液，其可以是醇，洗涤剂，氧化剂或表面活性剂，具有或不具有水，冲洗组织的空腔并去除和灭活病毒和细菌污染物。 在第二个实施方案中，将骨或结缔组织以分批方法离心以通过首先将组织干燥并然后将组织浸没在病毒/细菌清洁溶液中并从而将浸没的组织和溶液离心 从旋转轴线到足够高的速度和半径，以在组织和溶液上产生基本上高的G力，以迫使溶液进入和穿过组织腔。 然后从组织的附近去除具有冲洗的污染物的溶液。 在另一个实施方案中，将酶与组织离心以消化脂质和蛋白质，然后用​​冲洗溶液除去，所述冲洗溶液也可能使病毒和细菌污染物失活。 在又一个实施例中，离心机可用于将生物和/或结构上有用的材料注入组织。 此外，脂质和蛋白质可以通过化学活性剂如氧阴离子去除。