公开(公告)号：US20100144544A1

公开(公告)日：2010-06-10

申请号：US12522237

申请日：2008-01-04

申请人： George William Fraser ,  David J.M. Ray

发明人： George William Fraser ,  David J.M. Ray

IPC分类号： C40B30/04 ,  C40B40/06 ,  C40B50/00 ,  C40B60/12 ,  C07H21/00

CPC分类号： G01N21/6428 ,  G01N2021/6441

摘要： Fluorescence Labelling This invention generally relates to techniques for fluorescence labelling, and to methods, apparatus and computer program code for processing fluorescence signal data. A method of determining respective first and second degree-of-labelling signals for different respective first and second fluorophores associated with a common entity, the method comprising: determining a first fluorescence signal from said first and second fluorophores under first conditions; determining a second fluorescence signal from said first and second fluorophores under second conditions different to said first conditions; and determining said first and second degree-of-labelling signals for said first and second fluorophores from said first and second fluorescence signals; and wherein said determining of said first and second degree-of-labelling signals is responsive to at least one coupling value (c12; c21) representing a coupling of energy between said fluorophores.

摘要翻译： 荧光标记本发明一般涉及用于荧光标记的技术，以及用于处理荧光信号数据的方法，装置和计算机程序代码。 确定与共同实体相关联的不同的相应的第一和第二荧光团的相应的第一和第二标记信号的方法，所述方法包括：在第一条件下从所述第一和第二荧光团确定第一荧光信号; 在与所述第一条件不同的第二条件下，从所述第一和第二荧光团确定第二荧光信号; 以及从所述第一和第二荧光信号确定所述第一和第二荧光团的所述第一和第二标记信号; 并且其中所述确定所述第一和第二标记符号信号响应于表示所述荧光团之间的能量耦合的至少一个耦合值（c12; c21）。