公开(公告)号：US20120107959A1

公开(公告)日：2012-05-03

申请号：US13379345

申请日：2010-06-21

Applicant: Lance Liotta ,  Virginia Espina ,  Alessandra Luchini ,  Davide Tamburro

Inventor： Lance Liotta ,  Virginia Espina ,  Alessandra Luchini ,  Davide Tamburro

IPC: G01N21/75

CPC classification number: C07D311/82 ,  B01J20/3253 ,  B01J20/3255 ,  C07D251/50 ,  C07D279/20 ,  C07D403/04 ,  C07D471/22

Abstract: This invention describes the identification of novel organic dye chemistries that can be used as affinity baits to capture proteins and other biomolecules useful in the fields of medical diagnostics, environmental science, toxicology, and infectious disease. Incorporation of unique affinity dye compounds within hydrogel capture particles improves analyte yield and preanalytical precision, and stabilizes the analyte against degradation, while increasing measurement sensitivity. The particles in this invention can be used for routine clinical testing as well as for discovery of low abundance disease biomarkers. Example hydrogel particles containing new high affinity bait chemistries were used to identify a new set of human serum biomarkers.

Abstract translation: 本发明描述了可用作捕获蛋白质和其它可用于医学诊断学，环境科学，毒理学和传染病领域的其他生物分子的亲和诱饵的新型有机染料化学物质的鉴定。 在水凝胶捕获颗粒中引入独特的亲和染料化合物可提高分析物的产率和分析前精度，并稳定分析物以防止降解，同时提高测量灵敏度。 本发明中的颗粒可用于常规临床测试以及发现低丰度疾病生物标志物。 使用含有新的高亲和力诱饵化学物质的示例性水凝胶颗粒来鉴定新的一组人血清生物标志物。

公开(公告)号：US20130085076A1

公开(公告)日：2013-04-04

申请号：US13513568

申请日：2010-12-01

Applicant: Temple Douglas ,  Alessandra Luchini ,  Claudia Fredolini ,  Davide Tamburro ,  Lance Liotta

Inventor： Temple Douglas ,  Alessandra Luchini ,  Claudia Fredolini ,  Davide Tamburro ,  Lance Liotta

IPC: G01N33/569

CPC classification number: G01N33/56911 ,  G01N33/54313 ,  G01N2333/20 ,  G01N2469/10 ,  Y02A50/57

Abstract: The invention relates to both a sensitive method for the capture and detection of low-abundance Borrelia burgdorferi (Bb) bacterial antigens allowing for the diagnosis of Lyme Disease using standard immunoassays. Furthermore, this invention allows the antigen to be identified in a sample of urine, serum, or other biological fluids isolated from humans and animals. The invention provides a method to capture, concentrate, separate and specifically quantify the abundance of Bb antigens using immunoassays. The detection of Bb Outer Surface Protein A is presented as an example of the disclosed invention. High sensitivity levels, low cost and easily collected biofluids allow this technology to reach patients in clinics as well as POC applications for the early detection of Lyme disease prior to seroconversion. A kit containing necessary reagents and the method for diagnosis, monitoring or assessing lyme disease using an immunoassay such as an ELISA, western blot or RPPMA is disclosed.

Abstract translation: 本发明涉及用于捕获和检测低丰度伯氏疏螺旋体（Bb）细菌抗原的敏感方法，其允许使用标准免疫测定法诊断莱姆病。 此外，本发明允许在从人和动物分离的尿液，血清或其他生物流体的样品中鉴定抗原。 本发明提供了使用免疫测定法捕获，浓缩，分离和特异性定量Bb抗原丰度的方法。 Bb外表面蛋白A的检测作为本发明的一个例子。 高灵敏度水平，低成本和易于收集的生物流体使得该技术可以在诊所和POC应用中接触患者，以便在血清转化前早期发现莱姆病。 公开了含有必需试剂的试剂盒和使用免疫测定如ELISA，western印迹或RPPMA进行诊断，监测或评估淋巴细胞疾病的方法。