公开(公告)号：US12066436B2

公开(公告)日：2024-08-20

申请号：US17200553

申请日：2021-03-12

Applicant: QIAGEN Sciences LLC ,  Biopeptides Corp. ,  Gundersen Lutheran Medical Foundation, Inc.

Inventor： Steven M. Callister ,  Jeff Boyle ,  Misato Miyamasu ,  Raymond Dattwyler ,  Paul M. Arnaboldi

IPC: G01N33/569 ,  C07K14/20

CPC classification number: G01N33/569 ,  C07K14/20 ,  G01N2333/20 ,  G01N2800/52 ,  Y02A50/30

Abstract: Compositions and methods are provided for detection, diagnosis and prognosis of Lyme disease (LD), including a method for confirming Borrelia spp. infection by contacting, in vitro, whole blood samples from subjects suspected of having LD with synthetic peptides comprising T-cell epitope-containing regions derived from Borrelia proteins that are expressed at different stages of Lyme disease, and indirectly detecting LD-specific activated T-cells by determining production of a T-cell immune response indicator (e.g., interferon-Y) in response to stimulation by the peptides. Also disclosed are methods for predicting elimination of LD spirochetes in LD patients who have undergone LD treatment, by exposing whole blood samples from such subjects to peptides comprising specific T-cell epitope regions of Borrelia proteins that are expressed at different stages of Lyme disease, and confirming a lack of Borrelia-specific activated T-cells in the samples by the absence of a detectable T-cell immune response indicator (e.g., interferon-Y).

公开(公告)号：US20240077488A1

公开(公告)日：2024-03-07

申请号：US18270726

申请日：2021-12-29

Applicant: Mary Lape Nixon

Inventor： Mary Lape Nixon

IPC: G01N33/576 ,  G01N33/545 ,  G01N33/569

CPC classification number: G01N33/5765 ,  G01N33/545 ,  G01N33/56911 ,  G01N2333/08 ,  G01N2333/20 ,  G01N2469/20

Abstract: The present disclosure relates generally an immunoassay platform that utilizes a bead-based system to detect the presence or absence of antibodies in a test sample. The disclosed platform relates to methods and kits and can be used to detect the presence, absence, or exposure to Borrelia miyamotoi or Hepatitis D virus (HDV), or other pathogens or microbes of interest in a subject.

公开(公告)号：US11739388B2

公开(公告)日：2023-08-29

申请号：US16346178

申请日：2017-11-03

Applicant: UNIVERSITY OF LEICESTER ,  PHELIX RESEARCH AND DEVELOPMENT LIMITED

Inventor： Martha Rebecca Jane Clokie ,  Jinyu Shan ,  Louis Charles Teulieres

IPC: C12Q1/689 ,  C12Q1/6811 ,  C12Q1/6888 ,  G01N33/569

CPC classification number: C12Q1/689 ,  C12Q1/6811 ,  C12Q1/6888 ,  G01N33/56911 ,  G01N33/56983 ,  C12Q2600/112 ,  G01N2333/005 ,  G01N2333/20 ,  G01N2800/26 ,  Y02A50/30

Abstract: This invention relates to methods of detecting Borrelia burgdorferi sensu lato or for detecting Borrelia associated with Relapsing Fever (RF), kits for carrying out such methods, and methods of treating Borrelia burgdorferi sensu lato or RF infections in a subject. Uses of phage specific for Borrelia are also provided.

公开(公告)号：US20180275126A1

公开(公告)日：2018-09-27

申请号：US15976938

申请日：2018-05-11

Applicant: Virginia Commonwealth University

Inventor： Richard T. MARCONI ,  Christopher EARNHART ,  Lee D. OLIVER

IPC: G01N33/569 ,  C07K16/12 ,  A61K39/02

CPC classification number: G01N33/56911 ,  A61K39/0225 ,  A61K2039/55505 ,  A61K2039/55566 ,  A61K2039/70 ,  C07K14/20 ,  C07K16/1207 ,  C07K2317/34 ,  C07K2317/734 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/22 ,  G01N2333/20 ,  G01N2469/20 ,  Y02A50/401 ,  Y02A50/57

Abstract: Chimeric polyvalent recombinant proteins for use as vaccines and diagnostics for Lyme disease (e.g. in canines and humans) are provided. The chimeric proteins comprise epitopes of the loop 5 region and/or the alpha helix 5 region of outer surface protein C (OspC) types and/or OspE types. The OspC types may be associated with mammalian Borrelia infections.

公开(公告)号：US20180238874A1

公开(公告)日：2018-08-23

申请号：US15957312

申请日：2018-04-19

Applicant: BIOMERIEUX

Inventor： Lionel LEVET ,  Odile MEJAN-LETOURNEUR

IPC: G01N33/569 ,  C07K16/42 ,  C07K14/20

CPC classification number: G01N33/56911 ,  A61K39/00 ,  C07K14/195 ,  C07K14/20 ,  C07K16/4233 ,  C07K2319/40 ,  G01N2333/20 ,  G01N2469/20 ,  Y02A50/401 ,  Y02A50/57

Abstract: Chimera proteins includlng: (i) at least one sequence of a DbpA protein of a Borrelia species selected from B. afzelii, B. burgdorferi sensu stricto and B. garinii, and (ii) at least one sequence of an OspC protein of a Borrelia species selected from B. afzelii, B. burgdorferi sensu stricto and B. garinii. Also, a method and a kit for the in vitro diagnosis of Lyme borreliosis using said proteins.

公开(公告)号：US20170336409A1

公开(公告)日：2017-11-23

申请号：US15525823

申请日：2015-07-07

Applicant: VERAMARX, INC.

Inventor： Whitney RICHARDS ,  Floyd E. TAUB ,  Robert A. RUBIN

IPC: G01N33/569

CPC classification number: G01N33/56911 ,  G01N2333/20 ,  G01N2800/60 ,  Y02A50/57

Abstract: The present invention relates to methods for the diagnosing, prognosing, monitoring, differentiating, treating, and managing of Lyme disease in a subject. The methods according to the invention are characterized by the detection of a biomarker signature comprised of a combination of two or more analytes indicative of disease.

公开(公告)号：US09816991B2

公开(公告)日：2017-11-14

申请号：US14376409

申请日：2013-02-01

Applicant: Biopeptides Corp.

Inventor： Raymond J. Dattwyler ,  Paul M. Arnaboldi

IPC: A61K39/02 ,  A61K39/00 ,  A61K39/38 ,  G01N33/569 ,  C07K14/20

CPC classification number: G01N33/56911 ,  A61K39/02 ,  C07K14/20 ,  G01N2333/20 ,  Y02A50/57

Abstract: The present invention relates, e.g., to a composition comprising peptides represented by SEQ ID NO:1-SEQ ID NO:28 and/or SEQ ID NO:41-SEQ ID NO:47, or active variants thereof, wherein the peptides or active variants can bind specifically to an antibody induced by a causative agent of Lyme disease (a pathogenic Borrelia), e.g. in a sample from a subject having Lyme disease. Compositions of the invention may comprise multiple peptides, from multiple proteins. Diagnostic kits comprising the peptides are described, as are diagnostic assays using the peptides.

公开(公告)号：US09766238B2

公开(公告)日：2017-09-19

申请号：US14924358

申请日：2015-10-27

Applicant: ABAXIS, INC.

Inventor： Rajesh K. Mehra ,  Kenneth P. Aron ,  Dennis M. Bleile ,  Jeremy Walker ,  Cristina Cuesico

IPC: A61K39/00 ,  A61K39/02 ,  A61K39/38 ,  G01N33/569 ,  C07K14/20

CPC classification number: G01N33/56911 ,  C07K14/20 ,  G01N2333/20 ,  G01N2469/20 ,  Y02A50/57

Abstract: The invention provides compositions (e.g., peptide compositions) useful for the detection of antibodies that bind to Borrelia antigens. The peptide compositions comprise polypeptide sequences comprising variants in the IR6 domain of the Borrelia VlsE protein. The invention also provides devices, methods, and kits comprising such peptide compositions and useful for the detection of antibodies that bind to Borrelia antigens and the diagnosis of Lyme disease.

公开(公告)号：US20170114106A1

公开(公告)日：2017-04-27

申请号：US15361162

申请日：2016-11-25

Applicant: Christopher G. Earnhart ,  Richard T. Marconi

Inventor： Christopher G. Earnhart ,  Richard T. Marconi

IPC: C07K14/20 ,  C07K14/00

CPC classification number: C07K14/20 ,  A61K39/00 ,  A61K39/0225 ,  C07K14/001 ,  C07K16/1207 ,  C07K2319/00 ,  C07K2319/21 ,  G01N33/56911 ,  G01N2333/20 ,  G01N2469/20 ,  Y02A50/401 ,  Y02A50/57

Abstract: Antigenic polypeptides comprising linear immunodominant epitopes of Borrelia outer surface protein A (OspA) or Borrelia outer surface protein C (OspC) are useful as vaccines against Lyme disease, and as diagnostics for detecting Borrelia infections. The OspA and OspC antigenic polypeptides typically comprise a plurality of peptides representing epitope containing regions from multiple distinct phyletic groups. The antigenic polypeptides may also include epitopes from both Borrelia OspA and Borrelia OspC.

公开(公告)号：US20170030911A1

公开(公告)日：2017-02-02

申请号：US15200657

申请日：2016-07-01

Applicant: The United States of America, as represented by the Secretary, Department of Health and Human Serv

Inventor： Theresa M. Russell ,  Barbara J.B. Johnson

IPC: G01N33/573

CPC classification number: G01N33/573 ,  C07K16/12 ,  C07K2317/32 ,  G01N33/56911 ,  G01N33/581 ,  G01N33/6863 ,  G01N33/6869 ,  G01N2333/20 ,  G01N2333/952 ,  G01N2469/10 ,  G01N2469/20 ,  G01N2800/26 ,  G01N2800/52 ,  Y02A50/57

Abstract: Compositions and methods of the present invention relating to B. burgdorferi HtrA sensu lato (BbHtrA) protease activity, its substrates, cleavage products, biological effects and use in detection, diagnosis and/or treatment of Lyme disease are provided.

Abstract translation: 本发明的组合物和方法涉及Burgdorferi HtrA感染（BbHtrA）蛋白酶活性，其底物，切割产物，生物学效应以及在莱姆病的检测，诊断和/或治疗中的应用。