公开(公告)号：US08569463B2

公开(公告)日：2013-10-29

申请号：US12766063

申请日：2010-04-23

申请人： Yan-Yeung Luk ,  DaWei Cui ,  Debjyoti Bandyopadhyay ,  Deepali Prashar ,  Preeti Sejwal ,  Karen Simon

发明人： Yan-Yeung Luk ,  DaWei Cui ,  Debjyoti Bandyopadhyay ,  Deepali Prashar ,  Preeti Sejwal ,  Karen Simon

IPC分类号： C07K16/00 ,  C07K17/00 ,  C07K17/14 ,  C07K1/00 ,  C07K14/00 ,  C12P21/08 ,  C08H1/00

CPC分类号： C07K1/1077 ,  A61K38/00 ,  A61K39/39591 ,  A61K41/0004 ,  C07K1/006 ,  C07K1/13

摘要： A system and method for preventing protein aggregation is developed by covalent modification of proteins with organic molecules that can preserve the native protein folding. Proteins are covalently modified with sugar alcohols or cyclodextrins (organic Kosmotropes) or other small molecule drugs by water-driven bioorganic reactions in water. In the water-driven bioorganic reactions, the reagent is stable in water and can modify lysine residues or cysteine residue of a protein at physiological conditions with high yield and fast rate. Proteins and antibodies will be modified by non-natural sugar alcohols. As a result, the efficacy of protein drugs (reduction in aggregation and enzymatic degradation, and increase in blood stream life time) may be improved.

摘要翻译： 用蛋白质共价修饰可以保护天然蛋白质折叠的有机分子来发展蛋白质聚集的系统和方法。 通过水驱动的生物有机反应，蛋白质与糖醇或环糊精（有机Kosmotropes）或其他小分子药物共价修饰。 在水驱动的生物有机反应中，试剂在水中稳定，可以在生理条件下以高产率和快速率调节蛋白质的赖氨酸残基或半胱氨酸残基。 蛋白质和抗体将被非天然糖醇修饰。 结果，可以提高蛋白质药物的功效（聚集和酶降解降低，血流寿命增加）。

公开(公告)号：US20100273991A1

公开(公告)日：2010-10-28

申请号：US12766063

申请日：2010-04-23

申请人： Yan-Yeung Luk ,  DaWei Cui ,  Debjyoti Bandyopadhyay ,  Deepali Prashar ,  Preeti Sejwal ,  Karen Simon

发明人： Yan-Yeung Luk ,  DaWei Cui ,  Debjyoti Bandyopadhyay ,  Deepali Prashar ,  Preeti Sejwal ,  Karen Simon

IPC分类号： C07K16/00 ,  C07K1/107 ,  C07K14/00

CPC分类号： C07K1/1077 ,  A61K38/00 ,  A61K39/39591 ,  A61K41/0004 ,  C07K1/006 ,  C07K1/13

摘要： A system and method for preventing protein aggregation is developed by covalent modification of proteins with organic molecules that can preserve the native protein folding. Proteins are covalently modified with sugar alcohols or cyclodextrins (organic Kosmotropes) or other small molecule drugs by water-driven bioorganic reactions in water. In the water-driven bioorganic reactions, the reagent is stable in water and can modify lysine residues or cysteine residue of a protein at physiological conditions with high yield and fast rate. Proteins and antibodies will be modified by non-natural sugar alcohols. As a result, the efficacy of protein drugs (reduction in aggregation and enzymatic degradation, and increase in blood stream life time) may be improved.

摘要翻译： 用蛋白质共价修饰可以保护天然蛋白质折叠的有机分子来发展蛋白质聚集的系统和方法。 通过水驱动的生物有机反应，蛋白质与糖醇或环糊精（有机Kosmotropes）或其他小分子药物共价修饰。 在水驱动的生物有机反应中，试剂在水中稳定，可以在生理条件下以高产率和快速率调节蛋白质的赖氨酸残基或半胱氨酸残基。 蛋白质和抗体将被非天然糖醇修饰。 结果，可以提高蛋白质药物的功效（聚集和酶降解降低，血流寿命增加）。