公开(公告)号：US08784827B2

公开(公告)日：2014-07-22

申请号：US12991689

申请日：2008-05-09

申请人： Thomas Zürcher ,  Juan José Bernal ,  Cayetano von Kobbe ,  Ignacio Jiménez Torres ,  Ana Diaz Blázquez ,  Diana Martin Lorenzo ,  Gloria Calderita Lucas ,  Arcadio Garcia de Castro

发明人： Thomas Zürcher ,  Juan José Bernal ,  Cayetano von Kobbe ,  Ignacio Jiménez Torres ,  Ana Diaz Blázquez ,  Diana Martin Lorenzo ,  Gloria Calderita Lucas ,  Arcadio Garcia de Castro

IPC分类号： A61K39/00 ,  A61K39/12 ,  C07K14/005

CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K2039/5256 ,  A61K2039/5258 ,  C07K2319/40 ,  C12N2720/10022 ,  C12N2720/10023

摘要： The field of the invention refers to chimeric Virus Like Particles (VLP) derived from Birnavirus chimeric VP2 protein. In particular, the invention refers to chimeric VP2 fusion proteins which incorporate insertions and/or substitutions with one or more amino acids or particular peptide of interest while maintaining the capacity to assemble in the form of VLP. The invention identifies particular insertion and/or substitutions sites within VP2 P loop regions and outside said P loop regions. The invention also incorporates methods for the identification of preferred insertion and substitution sites within VP2 for the incorporation of particular amino acids and peptides of interest. The resulting chimeric VLP are of interest in the design of therapeutic and prophylactic vaccines as well as in the design of drug delivery systems, carriers for DNA and RNA in gene therapy, as targeted agents, in the development of antitoxins, and as diagnostic reagents.

摘要翻译： 本发明的领域是指衍生自Birnavirus嵌合VP2蛋白的嵌合病毒样颗粒（VLP）。 特别地，本发明涉及嵌合VP2融合蛋白，其掺入一个或多个氨基酸或特定感兴趣肽的插入和/或取代，同时保持以VLP形式组装的能力。 本发明鉴定VP2P环区域内的所述P环区域内的特定插入和/或取代位点。 本发明还包括用于鉴定VP2内优选的插入和取代位点以便并入特定的目的氨基酸和肽的方法。 所产生的嵌合VLP在治疗和预防性疫苗的设计以及药物递送系统的设计，基因治疗中的DNA和RNA的载体，靶向剂，抗毒素的开发和诊断试剂方面都是感兴趣的。

公开(公告)号：US20110190164A1

公开(公告)日：2011-08-04

申请号：US12991689

申请日：2008-05-09

申请人： Thomas Zurcher ,  Juan Jose Bernal ,  Cayetano von Kobbe ,  Ignacio JimenezTorres ,  Ana Diaz Blázquez ,  Diana Martin Lorenzo ,  Gloria Calderita Lucas ,  Arcadio Garcia de Castro

发明人： Thomas Zurcher ,  Juan Jose Bernal ,  Cayetano von Kobbe ,  Ignacio JimenezTorres ,  Ana Diaz Blázquez ,  Diana Martin Lorenzo ,  Gloria Calderita Lucas ,  Arcadio Garcia de Castro

IPC分类号： C40B30/06 ,  C07K19/00 ,  C07H21/00 ,  C12N15/63 ,  C40B40/02 ,  C12P21/00 ,  C12N1/00 ,  C12N5/10

CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K2039/5256 ,  A61K2039/5258 ,  C07K2319/40 ,  C12N2720/10022 ,  C12N2720/10023

摘要： The field of the invention refers to chimeric Virus Like Particles (VLP) derived from Birnavirus chimeric VP2 protein. In particular, the invention refers to chimeric VP2 fusion proteins which incorporate insertions and/or substitutions with one or more amino acids or particular peptide of interest while maintaining the capacity to assemble in the form of VLP. The invention identifies particular insertion and/or substitutions sites within VP2 P loop regions and outside said P loop regions. The invention also incorporates methods for the identification of preferred insertion and substitution sites within VP2 for the incorporation of particular amino acids and peptides of interest. The resulting chimeric VLP are of interest in the design of therapeutic and prophylactic vaccines as well as in the design of drug delivery systems, carriers for DNA and RNA in gene therapy, as targeted agents, in the development of antitoxins, and as diagnostic reagents.

摘要翻译： 本发明的领域是指衍生自Birnavirus嵌合VP2蛋白的嵌合病毒样颗粒（VLP）。 特别地，本发明涉及嵌合VP2融合蛋白，其掺入一个或多个氨基酸或特定感兴趣肽的插入和/或取代，同时保持以VLP形式组装的能力。 本发明鉴定VP2P环区域内的所述P环区域内的特定插入和/或取代位点。 本发明还包括用于鉴定VP2内优选的插入和取代位点以便并入特定的目的氨基酸和肽的方法。 所产生的嵌合VLP在治疗和预防性疫苗的设计以及药物递送系统的设计，基因治疗中的DNA和RNA的载体，靶向剂，抗毒素的开发和诊断试剂方面都是感兴趣的。