公开(公告)号：US20130212718A1

公开(公告)日：2013-08-15

申请号：US13852379

申请日：2013-03-28

申请人： Malcolm James FRASER ,  Randy LEWIS ,  Don JARVIS ,  Kimberly THOMPSON ,  Joseph HULL ,  Yun-Gen MIAO ,  Florence TEULE ,  Bonghee SOHN ,  Youngsoo KIM

发明人： Malcolm James FRASER ,  Randy LEWIS ,  Don JARVIS ,  Kimberly THOMPSON ,  Joseph HULL ,  Yun-Gen MIAO ,  Florence TEULE ,  Bonghee SOHN ,  Youngsoo KIM

IPC分类号： C07K14/435

CPC分类号： C07K14/43586 ,  A01K67/0333 ,  A01K67/04 ,  A01K2217/052 ,  A01K2227/706 ,  A01K2267/01 ,  A01K2267/02 ,  A61K38/00 ,  C07K14/43518 ,  C07K2319/00

摘要： Transgenic silkworms comprising at least one nucleic acid encoding a chimeric silk polypeptide comprising one or more spider silk elasticity and strength motifs are disclosed. Expression cassettes comprising nucleic acids encoding a variety of chimeric spider silk polypeptides (Spider 2, Spider 4, Spider 6, Spider 8) are also disclosed. A piggyBac vector system is used to incorporate nucleic acids encoding chimeric spider silk polypeptides into the mutant silkworms to generate stable transgenic silkworms. Chimeric silk fibers having improved tensile strength and elasticity characteristics compared to native silkworm silk fibers are also provided. The transgenic silkworms greatly facilitate the commercial production of chimeric silk fibers suitable for use in a wide variety of medical and industrial applications.

摘要翻译： 公开了包含编码包含一种或多种蜘蛛丝弹性和强度基序的嵌合丝多肽的至少一种核酸的转基因蚕。 还公开了包含编码多种嵌合蜘蛛丝多肽（Spider 2，Spider 4，Spider 6，Spider 8）的核酸的表达盒。 使用piggyBac载体系统将编码嵌合蜘蛛丝多肽的核酸掺入突变家蚕中以产生稳定的转基因蚕。 还提供了与天然蚕丝纤维相比具有改善的拉伸强度和弹性特征的嵌合丝纤维。 转基因家蚕大大促进了适用于各种医疗和工业应用的嵌合蚕丝纤维的商业生产。