公开(公告)号：US12037601B2

公开(公告)日：2024-07-16

申请号：US16082157

申请日：2017-03-03

Applicant: Indoor Biotechnologies Inc.

Inventor： Martin D. Chapman ,  Olya Spassibojko

IPC: C12N5/00 ,  A01K67/0276 ,  C12N15/11 ,  C12N15/90

CPC classification number: C12N15/907 ,  A01K67/0276 ,  C12N15/11 ,  A01K2217/075 ,  A01K2227/10 ,  A01K2267/02 ,  C12N2310/20 ,  C12N2800/80

Abstract: Compositions and methods for genetically modifying felines or feline cells are described. The compositions and methods are useful for knocking out all or a portion of a Fel d 1 gene from a feline genome. Feline cells and organisms in which all or a portion of the Fel d 1 gene is knocked out are also described. The compositions and methods may include reagents and procedures for CRISPR-Cas9-mediated genomic editing of Fel d 1.

公开(公告)号：US20230416783A1

公开(公告)日：2023-12-28

申请号：US18011704

申请日：2021-06-23

Applicant: The Regents of the University of California

Inventor： Omar Akbari ,  Anna Buchman

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/11 ,  C07K14/47 ,  A01K67/033

CPC classification number: C12N15/902 ,  C12N9/22 ,  C12N15/111 ,  C07K14/4705 ,  A01K67/0339 ,  C12N2310/20 ,  A01K2227/706 ,  A01K2217/072 ,  A01K2267/02

Abstract: Provided herein are methods of engineering a reproductive barrier in an insect including (a) introducing into a first insect a first nucleic acid sequence, where the first nucleic acid sequence targets a genomic sequence, where the genomic sequence is proximal to a transcription start site of a gene product; and (b) introducing into a second insect a second nucleic acid sequence encoding a nuclease-deficient endonuclease; and (c) genetically crossing the first insect with the second insect, where the second insect includes a second copy of the genomic sequence where the second copy of the genomic sequence is proximal to a transcription start site of a second copy of the gene product.

公开(公告)号：US20230354790A1

公开(公告)日：2023-11-09

申请号：US18246250

申请日：2021-09-28

Applicant: Brandeis University

Inventor： Paul Allen Garrity ,  Willem Jeffrey Laursen

IPC: A01K67/033 ,  C12N9/22 ,  C12N15/11 ,  C12N15/90 ,  C07K14/435

CPC classification number: A01K67/0339 ,  C12N9/22 ,  C12N15/111 ,  C12N15/902 ,  C07K14/43563 ,  C12N2310/20 ,  C12N2800/80 ,  A01K2227/706 ,  A01K2217/072 ,  A01K2267/02

Abstract: A method of making sterile diploid organisms includes mating a first population and a second population of single knock-in diploid organisms, wherein the first population of single knock-in diploid organisms are heterozygous organisms expressing a first marker inserted into a gene required for fertility, wherein the second population of single knock-in diploid organisms are heterozygous organisms expressing a second marker inserted into the gene required for fertility, wherein introduction of the first/second marker disrupts expression of the required fertility gene creating a first/second mutant allele of the gene required for fertility, and wherein the first and second markers are distinct; sorting offspring produced from the mating based on their expression of the first and/or second markers; and isolating the sterile diploid organisms, wherein the sterile diploid organisms are heteroallelic diploid organisms expressing the first marker in the first mutant allele and the second marker in the mutant second allele.

公开(公告)号：US11716975B2

公开(公告)日：2023-08-08

申请号：US17011461

申请日：2020-09-03

Applicant: GloFish LLC

Inventor： Alan Blake ,  Richard Crockett ,  Aidas Nasevicius

IPC: A01K67/027

CPC classification number: A01K67/0275 ,  A01K2217/052 ,  A01K2227/40 ,  A01K2267/02

Abstract: The present invention relates to transgenic blue ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.

公开(公告)号：US20190216064A1

公开(公告)日：2019-07-18

申请号：US16307982

申请日：2017-06-09

Applicant: VANDERBILT UNIVERSITY ,  YALE UNIVERSITY

Inventor： Jason METCALF ,  Seth R. BORDENSTEIN ,  Daniel LEPAGE ,  Sarah BORDENSTEIN ,  Mark HOCHSTRASSER ,  John F. BECKMANN ,  Judith RONAU

IPC: A01K67/033 ,  C12R1/01 ,  C12N9/64 ,  C07K14/195 ,  C12N15/87 ,  C12N9/22

CPC classification number: A01K67/0337 ,  A01K67/0339 ,  A01K2217/052 ,  A01K2217/206 ,  A01K2227/706 ,  A01K2267/02 ,  C07K14/195 ,  C07K2319/21 ,  C12N9/22 ,  C12N9/6472 ,  C12N15/87 ,  C12R1/01

Abstract: The disclose relates to genetically modified bacteria, genetically modified arthropods, and methods for controlling and/or reducing arthropod populations.

公开(公告)号：US20190216063A1

公开(公告)日：2019-07-18

申请号：US16371824

申请日：2019-04-01

Applicant: The University Court of the University of Edinburgh

Inventor： Christopher Bruce Alexander Whitelaw ,  Christopher James Palgrave ,  Simon Geoffrey Lillico

IPC: A01K67/027 ,  C12N9/22 ,  C07K14/47

CPC classification number: A01K67/0276 ,  A01K67/0275 ,  A01K2217/07 ,  A01K2217/072 ,  A01K2217/075 ,  A01K2227/108 ,  A01K2267/02 ,  A01K2267/03 ,  C07K14/4702 ,  C12N9/22

Abstract: The present invention relates to a genetically edited animal, especially to a genetically edited pig in which expression or activity of the RELA protein has been modified. Such pigs have at least partial protection against the African Swine Fever Virus. The invention also provides, a cell nucleus, germ cell, stem cell, gamete, blastocyst, embryo, foetus and/or donor cell of a non-human animal comprising a genetic modification which alters the expression or function of RELA protein, methods for editing the genome of animals and methods for screening the efficacy of a pharmaceutical agent in such an animal.

公开(公告)号：US09968077B2

公开(公告)日：2018-05-15

申请号：US14933031

申请日：2015-11-05

Applicant: GloFish, LLC

Inventor： Alan Blake ,  Richard Crockett ,  Aidas Nasevicius

IPC: A01K67/02 ,  A01K67/027

CPC classification number: A01K67/0275 ,  A01K2217/052 ,  A01K2227/40 ,  A01K2267/02

Abstract: The present invention relates to transgenic blue ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.

公开(公告)号：US09777331B2

公开(公告)日：2017-10-03

申请号：US14754729

申请日：2015-06-30

Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION

Inventor： Hasan Khatib

IPC: C12Q1/68 ,  A01K67/02 ,  A01K67/027 ,  A61D19/04 ,  A61K35/54

CPC classification number: C12Q1/6883 ,  A01K67/02 ,  A01K67/027 ,  A01K2227/101 ,  A01K2267/02 ,  A61D19/04 ,  A61K35/54 ,  C12Q1/68 ,  C12Q1/6888 ,  C12Q2600/124 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: Single nucleotide polymorphic sites of the bovine MAP1B, PPP1R11, and DDX4 genes are associated with improved bull fertility as measured by e.g. sire conception rates. Nucleic acid molecules, arrays, kits, methods of genotyping and marker-assisted bovine breeding methods based on these SNPs are disclosed.

公开(公告)号：US09763430B2

公开(公告)日：2017-09-19

申请号：US13738704

申请日：2013-01-10

Applicant: Zhiyuan Gong ,  Jiangyan He ,  Bensheng Ju ,  Toong Jin Lam ,  Yanfei Xu ,  Tie Yan

Inventor： Zhiyuan Gong ,  Jiangyan He ,  Bensheng Ju ,  Toong Jin Lam ,  Yanfei Xu ,  Tie Yan

IPC: A01K67/027 ,  C07K14/435 ,  C12N15/85 ,  G01N33/74

CPC classification number: A01K67/0275 ,  A01K2217/05 ,  A01K2217/20 ,  A01K2227/40 ,  A01K2267/02 ,  A01K2267/0393 ,  C07K14/43595 ,  C12N15/85 ,  C12N15/8509 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2830/80 ,  C12N2840/20 ,  G01N33/743

Abstract: Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed respectively, were isolated and ligated to the 5′ end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultraviolet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patterns, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.

公开(公告)号：US20170258834A1

公开(公告)日：2017-09-14

申请号：US15456656

申请日：2017-03-13

Applicant: Veritas Therapeutics Inc.

Inventor： Gary A. Levy ,  David Grant ,  Andrzej Chruscinski

IPC: A61K35/17 ,  A61K45/06 ,  C07K14/47 ,  A01K67/027

CPC classification number: A61K35/17 ,  A01K67/0275 ,  A01K2217/052 ,  A01K2227/105 ,  A01K2267/02 ,  A61K2035/124 ,  C07K14/75 ,  C12N9/6432 ,  C12N2800/30

Abstract: This application relates to methods and compositions for inducing immune tolerance. Specifically, methods and uses of regulatory T cells (Treg) and associated compositions for the induction of immune tolerance are described. The methods and uses may be used to prevent transplant rejection, and in the treatment of diseases or conditions such as graft versus host disease, autoimmune disease and allergies. Also provided are transgenic mice that ubiquitously express FGL2 protein from which the Treg may be isolated.