公开(公告)号：US20240254173A1

公开(公告)日：2024-08-01

申请号：US18018973

申请日：2022-10-11

申请人： WENZHOU MUQING BIOTECHNOLOGY CO., LTD

发明人： Shixuan Chen ,  Hua Xu ,  Hao Pan

IPC分类号： C07K14/435

CPC分类号： C07K14/43586

摘要： The present disclosure provides a method for sericin removal. Since sodium borohydride has a high hydrogen storage performance, a convenient hydrogen release, and relatively-stable chemical properties, the sodium borohydride in a sodium borohydride aqueous solution reacts with water slowly to release hydrogen. A large number of accumulated hydrogen bubbles pass through silk fibers, and produce an upward pulling force to the sericin on a fiber surface along the silk fibers under the action of inertial force and surface tension, thereby achieving the sericin removal at room temperature. Moreover, the method for sericin removal has a simple process, a complete sericin removal effect, and no damage to the structure of silk fibroin fibers, and can prepare the silk fibroin fibers with high flexibility and bright color.

公开(公告)号：US20240150416A1

公开(公告)日：2024-05-09

申请号：US18341669

申请日：2023-06-26

申请人： BOLT THREADS, INC.

发明人： Joshua Kittleson ,  Thomas Stevens ,  Rena Hill ,  Carlos Gustavo Pesce ,  David N. Breslauer ,  Daniel M. Widmaier

IPC分类号： C07K14/435 ,  C07K14/395 ,  C12P21/02

CPC分类号： C07K14/43586 ,  C07K14/395 ,  C12P21/02 ,  C07K2319/02

摘要： The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include expression constructs, recombinant vectors, and recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to recombinant secretion signals that comprise the leader peptide of the α-mating factor (αMF) of Saccharomyces cerevisiae and a non-αMF signal peptide.

公开(公告)号：US20240025944A1

公开(公告)日：2024-01-25

申请号：US18481313

申请日：2023-10-05

申请人： COCOON BIOTECH INC.

发明人： Scott Delisle ,  Michael Santos

IPC分类号： C07K1/34 ,  C07K1/36 ,  C07K14/435

CPC分类号： C07K1/34 ,  C07K1/36 ,  C07K14/43586

摘要： Described herein is a method of purifying a silk fibroin preparation including preparing an aqueous silk fibroin solution having a concentration of greater than or equal to 5% w/v silk fibroin from the silk fibroin preparation, wherein the silk fibroin preparation includes a chaotropic salt; and exchanging the chaotropic salt from the silk fibroin solution at a pH below the isoelectric point of the silk fibroin, wherein the pH is between 2 and 5, replacing the chaotropic salt with a buffer comprising 10 to 300 mM of a second salt, or a combination thereof, to prepare the purified silk fibroin.

公开(公告)号：US11667683B2

公开(公告)日：2023-06-06

申请号：US17245818

申请日：2021-04-30

申请人： AMSILK GmbH ,  Ludwig-Maximilians-Universitat Munchen ,  Universitat Bayreuth

发明人： Lin Romer ,  Ute Slotta ,  Julia Engert ,  Gerhard Winter ,  Matthias Lucke ,  Thomas Scheibel

IPC分类号： A61K39/00 ,  C07K14/435 ,  A61K39/385 ,  A61K47/64 ,  A61K47/65 ,  A61P35/00 ,  A61K47/42 ,  C12N15/62 ,  A61K38/00

CPC分类号： C07K14/43518 ,  A61K39/385 ,  A61K47/42 ,  A61K47/646 ,  A61K47/65 ,  A61P35/00 ,  C07K14/43586 ,  C12N15/62 ,  A61K38/00 ,  A61K2039/6031 ,  A61K2039/627 ,  C07K2319/40 ,  C07K2319/50

摘要： The present invention relates to a polypeptide comprising a silk polypeptide and an antigen. Further, the present invention relates to an article comprising the polypeptide. Furthermore, the present invention relates to a pharmaceutical composition comprising the article. In addition, the present invention relates to the article or pharmaceutical composition for use as a pharmaceutical, for inducing an immune response and/or for use in a prophylactic and/or therapeutic treatment of a disease.

公开(公告)号：US20180355120A1

公开(公告)日：2018-12-13

申请号：US15780477

申请日：2016-11-29

申请人： Spiber Inc. ,  Kojima Industries Corporation ,  Tekunohama Co., Ltd.

发明人： Koichi Kotaka ,  Takaoki Ishii ,  Junichi Sugahara ,  Ryota Sato ,  Junichi Noba

IPC分类号： C08J3/02 ,  C07K14/435 ,  C07K1/14

CPC分类号： C08J3/02 ,  C07K1/14 ,  C07K14/435 ,  C07K14/43586 ,  C08L89/00

摘要： Provided is a method for producing a protein solution, including applying a pressure to a dispersion liquid containing a protein and a polar solvent in which the protein is dispersed, to obtain a protein solution containing the protein and the polar solvent in which the protein is dissolved.

公开(公告)号：US10010574B2

公开(公告)日：2018-07-03

申请号：US11572944

申请日：2004-07-31

申请人： Sung Su Kim ,  Cheol Hyoung Park ,  Sang-Hyung Lee ,  Wan-Seok Joo ,  Won Bok Lee

发明人： Sung Su Kim ,  Cheol Hyoung Park ,  Sang-Hyung Lee ,  Wan-Seok Joo ,  Won Bok Lee

IPC分类号： A61P25/28 ,  A61P25/16 ,  A61K38/01 ,  C07K14/435

CPC分类号： A61K38/011 ,  C07K14/43586

摘要： A silk peptide having neuroprotective and neurofunctional activities and its preparation method are discussed. One method relates to preparing silk protein preferably having neuroprotective activity with weight average molecular weight of 200-100,000 by hydrolysis of silk fibroin; also discussed are a composition for preventing or treating brain disease comprising silk peptide and pharmaceutically acceptable carrier, and a composition for improving brain function.

公开(公告)号：US20180008522A1

公开(公告)日：2018-01-11

申请号：US15543029

申请日：2016-01-12

申请人： Silk Therapeutics, Inc.

发明人： Gregory H. Altman ,  Dylan S. Haas ,  Rebecca L. Horan

IPC分类号： A61K8/64 ,  A61Q19/02 ,  A61Q17/04 ,  A61K47/42 ,  A61K47/22 ,  A61K31/522 ,  A61K9/70 ,  A61K9/00 ,  A61K8/92 ,  A61K8/67 ,  A61K8/368 ,  A61Q19/08 ,  A61K8/04

CPC分类号： A61K8/64 ,  A61K8/0216 ,  A61K8/022 ,  A61K8/042 ,  A61K8/365 ,  A61K8/368 ,  A61K8/4973 ,  A61K8/676 ,  A61K8/922 ,  A61K8/97 ,  A61K9/0014 ,  A61K9/7007 ,  A61K31/522 ,  A61K47/22 ,  A61K47/42 ,  A61K2800/524 ,  A61Q17/005 ,  A61Q17/04 ,  A61Q19/00 ,  A61Q19/02 ,  A61Q19/08 ,  C07K14/43586

摘要： In some embodiments, a composition is disclosed that includes pure silk fibroin-based protein fragments that are substantially devoid of sericin, wherein the composition has an average weight average molecular weight ranging from about 17 kDa to about 38 kDa, wherein the composition has a polydispersity of between about 1.5 and about 3.0, wherein the composition is substantially homogeneous, wherein the composition between 0 ppm to about 500 ppm of inorganic residuals, and wherein the composition includes between 0 ppm to about 500 ppm of organic residuals. In some embodiments, a composition is disclosed that includes silk fibroin-based protein fragments and is suitable for use in consumer applications. In some embodiments, a preserved composition is disclosed that includes silk fibroin-based protein fragments. In some embodiments, a method of preserving a solution using silk fibroin-based protein fragments is disclosed.

公开(公告)号：US09623147B2

公开(公告)日：2017-04-18

申请号：US14293308

申请日：2014-06-02

申请人： Trustees of Tufts College

发明人： David L. Kaplan ,  Ung-Jin Kim ,  Jaehyung Park ,  Hyoung-Joon Jin

IPC分类号： A61L27/22 ,  A61L27/36 ,  A61L27/38 ,  A61L27/52 ,  C07K14/435 ,  C08J3/07 ,  C08J3/075 ,  C08J5/18 ,  C08J9/00 ,  C08J9/26 ,  C08J9/28 ,  C08L89/00 ,  D01D5/00 ,  D01F4/02 ,  B29C39/00 ,  B29C39/20 ,  B29C55/00 ,  B29D7/01 ,  B29L7/00 ,  B29L9/00

CPC分类号： A61L27/227 ,  A61L27/3604 ,  A61L27/38 ,  A61L27/52 ,  A61L27/56 ,  A61L2430/02 ,  B29C39/003 ,  B29C39/203 ,  B29C55/005 ,  B29D7/01 ,  B29K2089/00 ,  B29L2007/00 ,  B29L2009/00 ,  C07K14/43536 ,  C07K14/43586 ,  C08J3/07 ,  C08J3/075 ,  C08J5/18 ,  C08J9/0061 ,  C08J9/26 ,  C08J9/28 ,  C08J2201/0444 ,  C08J2201/0504 ,  C08J2205/022 ,  C08J2389/00 ,  C08L89/00 ,  D01D5/0007 ,  D01F4/02 ,  Y10T428/249921 ,  Y10T442/10 ,  Y10T442/60

摘要： The present invention provides for concentrated aqueous silk fibroin solutions and an all-aqueous mode for preparation of concentrated aqueous fibroin solutions that avoids the use of organic solvents, direct additives, or harsh chemicals. The invention further provides for the use of these solutions in production of materials, e.g., fibers, films, foams, meshes, scaffolds and hydrogels.

公开(公告)号：US09580697B2

公开(公告)日：2017-02-28

申请号：US15222830

申请日：2016-07-28

申请人： DNA2.0, INC.

发明人： Jeremy Minshull ,  Mark Welch ,  Sridhar Govindrajan ,  Kate Caves

IPC分类号： C07K16/00 ,  C12N15/85 ,  A61K31/7105 ,  A61K38/00 ,  C12N9/12 ,  C07K2/00

CPC分类号： C12N9/1241 ,  A61K31/00 ,  A61K31/7105 ,  A61K38/00 ,  C07K2/00 ,  C07K14/43586 ,  C07K14/463 ,  C07K16/00 ,  C07K16/32 ,  C07K2317/10 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2319/09 ,  C07K2319/80 ,  C12N15/85 ,  C12N15/907 ,  C12N2800/90 ,  C12N2830/40 ,  C12N2830/60 ,  C12Y207/07

摘要： The present invention provides polynucleotide vectors for high expression of heterologous genes, and methods for constructing such vectors. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, but not limited to, gene expression, gene therapy, insertional mutagenesis, or gene discovery.

摘要翻译： 本发明提供用于高表达异源基因的多核苷酸载体，以及用于构建此类载体的方法。 一些载体还包含进一步改善表达的新型转座子和转座酶。 进一步公开的是可用于稳定地将核酸引入细胞DNA的基因转移系统中的载体。 基因转移系统可以用于例如但不限于基因表达，基因治疗，插入诱变或基因发现的方法中。

公开(公告)号：US09517191B2

公开(公告)日：2016-12-13

申请号：US14876799

申请日：2015-10-06

申请人： Silk Therapeutics, Inc.

发明人： Gregory H. Altman ,  Rebecca L. Horan ,  Rachel Lee Dow ,  Rachel M. Lind ,  Dylan S. Haas

IPC分类号： A61K9/08 ,  A61K8/02 ,  A61K38/01 ,  A61K8/64 ,  C07K1/12 ,  C07K14/435 ,  A61K38/00 ,  A61K8/27 ,  A61K8/29 ,  A61K8/67 ,  A61K8/73 ,  A61K8/97 ,  A61Q17/04 ,  A61Q19/00 ,  A61Q19/08 ,  A45D33/00 ,  A61Q19/02 ,  A61K8/92 ,  A61K8/04 ,  A61K8/19 ,  A61K8/365 ,  A61K8/49 ,  A61K8/98

CPC分类号： A61K8/64 ,  A45D33/00 ,  A61K8/02 ,  A61K8/0216 ,  A61K8/042 ,  A61K8/19 ,  A61K8/27 ,  A61K8/29 ,  A61K8/365 ,  A61K8/4953 ,  A61K8/676 ,  A61K8/735 ,  A61K8/922 ,  A61K8/97 ,  A61K8/987 ,  A61K9/08 ,  A61K38/00 ,  A61K38/012 ,  A61K2800/591 ,  A61K2800/72 ,  A61K2800/74 ,  A61K2800/75 ,  A61Q17/04 ,  A61Q19/00 ,  A61Q19/007 ,  A61Q19/02 ,  A61Q19/08 ,  C07K1/12 ,  C07K14/43586

摘要： A composition is disclosed that includes pure silk fibroin-based protein fragments that are substantially devoid of sericin, wherein the composition has an average weight average molecular weight ranging from about 17 kDa to about 38 kDa, wherein the composition has a polydispersity of between about 1.5 and about 3.0, wherein the composition is substantially homogeneous, wherein the composition between 0 ppm to about 500 ppm of inorganic residuals, and wherein the composition includes between 0 ppm to about 500 ppm of organic residuals.