公开(公告)号：US07427380B2

公开(公告)日：2008-09-23

申请号：US10836425

申请日：2004-05-03

申请人： John A. McNeil ,  Michael Anthony Akong ,  Donald James Mierzeski ,  Gonul Velicelebi ,  David Philip Karlton

发明人： John A. McNeil ,  Michael Anthony Akong ,  Donald James Mierzeski ,  Gonul Velicelebi ,  David Philip Karlton

IPC分类号： B01L3/00

CPC分类号： G01N35/0099 ,  B01J2219/00315 ,  B01J2219/00585 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00702 ,  C40B60/14 ,  G01N33/5302 ,  G01N33/5304 ,  G01N35/0092 ,  G01N35/028 ,  G01N35/1065 ,  G01N2035/00752 ,  G01N2035/0093 ,  Y10T436/115831

摘要： An automated detection system includes a robotic fluid handling system for performing a plurality of assays; a detection system for imaging the assays as they are performed; and a computer control, data acquisition, and data analysis system for controlling the operation of the fluid-handling and detection system and for collecting and analyzing imaging data. The automated detection system comprises a pipettor for simultaneously distributing a predetermined amount of a liquid and a signal-generating element to each of the plurality of wells; an excitation source for simultaneously exposing the wells to excitation radiation; a detector for simultaneously detecting signal emitted from each of the wells over a period of time comprising a single imager for optically imaging the plurality of wells simultaneously; and computerized controller for simultaneously coordinating the pipettor, the excitation source, and the detector. Further, a method for performing simultaneous assays includes the steps of simultaneously distributing a predetermined amount of a liquid solution to each of a plurality of samples, simultaneously exposing the wells to excitation radiation, simultaneously detecting signals emitted from the wells using a detector comprising a single imaging means, and simultaneously controlling and coordinating the distribution, excitation, and detection using a computerized controller.

摘要翻译： 一种自动化检测系统，包括用于执行多个测定的机器人流体处理系统; 用于在进行测定时对分析进行成像的检测系统; 以及用于控制流体处理和检测系统的操作并用于收集和分析成像数据的计算机控制，数据采集和数据分析系统。 该自动检测系统包括移液器，用于同时将预定量的液体和信号产生元件分配到多个孔中的每一个; 用于同时将阱暴露于激发辐射的激发源; 检测器，用于同时检测在一段时间内从每个孔发射的信号，包括单个成像器，用于同时对多个孔进行光学成像; 和计算机控制器，用于同时协调移液器，激发源和检测器。 此外，用于执行同时测定的方法包括以下步骤：将预定量的液体溶液分配到多个样品中的每一个，同时将孔暴露于激发辐射，同时使用检测器检测从孔发射的信号，所述检测器包括单个 成像装置，并且使用计算机控制器同时控制和协调分布，激励和检测。

公开(公告)号：US06800452B1

公开(公告)日：2004-10-05

申请号：US08287358

申请日：1994-08-08

申请人： John Austin McNeil ,  Michael Anthony Akong ,  Donald James Mierzeski ,  Gonul Velicelebi ,  David Philip Karlton

发明人： John Austin McNeil ,  Michael Anthony Akong ,  Donald James Mierzeski ,  Gonul Velicelebi ,  David Philip Karlton

IPC分类号： C12Q102

CPC分类号： G01N35/0099 ,  B01J2219/00315 ,  B01J2219/00585 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00702 ,  C40B60/14 ,  G01N33/5302 ,  G01N33/5304 ,  G01N35/0092 ,  G01N35/028 ,  G01N35/1065 ,  G01N2035/00752 ,  G01N2035/0093 ,  Y10T436/115831

摘要： An automated detection system includes a robotic fluid handling system for performing a plurality of assays; a detection system for imaging the assays as they are performed; and a computer control, data acquisition, and data analysis system for controlling the operation of the fluid-handling and detection system and for collecting and analyzing imaging data. The automated detection system comprises a pipettor for simultaneously distributing a predetermined amount of a liquid and a signal-generating element to each of the plurality of wells; an excitation source for simultaneously exposing the wells to excitation radiation; a detector for simultaneously detecting signal emitted from each of the wells over a period of time comprising a single imager for optically imaging the plurality of wells simultaneously; and computerized controller for simultaneously coordinating the pipettor, the excitation source, and the detector. Further, a method for performing simultaneous assays includes the steps of simultaneously distributing a predetermined amount of a liquid solution to each of a plurality of samples, simultaneously exposing the wells to excitation radiation, simultaneously detecting signals emitted from the wells using a detector comprising a single imaging means, and simultaneously controlling and coordinating the distribution, excitation, and detection using a computerized controller.

摘要翻译： 一种自动化检测系统，包括用于执行多个测定的机器人流体处理系统; 用于在进行测定时对分析进行成像的检测系统; 以及用于控制流体处理和检测系统的操作并用于收集和分析成像数据的计算机控制，数据采集和数据分析系统。 该自动检测系统包括移液器，用于同时将预定量的液体和信号产生元件分配到多个孔中的每一个; 用于同时将阱暴露于激发辐射的激发源; 检测器，用于同时检测在一段时间内从每个孔发射的信号，包括单个成像器，用于同时对多个孔进行光学成像; 和计算机控制器，用于同时协调移液器，激发源和检测器。 此外，用于执行同时测定的方法包括以下步骤：将预定量的液体溶液分配到多个样品中的每一个，同时将孔暴露于激发辐射，同时使用检测器检测从孔发射的信号，所述检测器包括单个 成像装置，并且使用计算机控制器同时控制和协调分布，激励和检测。

公开(公告)号：US06746864B1

公开(公告)日：2004-06-08

申请号：US08465354

申请日：1995-06-05

申请人： John Austin McNeil ,  Michael Anthony Akong ,  Donald James Mierzeski ,  Gonul Velicelebi ,  David Philip Karlton

发明人： John Austin McNeil ,  Michael Anthony Akong ,  Donald James Mierzeski ,  Gonul Velicelebi ,  David Philip Karlton

IPC分类号： C12M134

CPC分类号： G01N35/0099 ,  B01J2219/00315 ,  B01J2219/00585 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00702 ,  C40B60/14 ,  G01N33/5302 ,  G01N33/5304 ,  G01N35/0092 ,  G01N35/028 ,  G01N35/1065 ,  G01N2035/00752 ,  G01N2035/0093 ,  Y10T436/115831

摘要： An automated detection system includes a robotic fluid handling system for performing assays on the plurality of test samples; a detection system for imaging the assays as they are performed; and a computer control, data acquisition, and data analysis system for controlling the operation of the detection system and for collecting and analyzing imaging data. The automated detection system comprises a pipettor for simultaneously distributing a predetermined amount of a reagent to each of the plurality of samples containing a fluorescent dye or other indicator moiety; an excitation source for simultaneously exciting a fluorescent reaction of the fluorescent dye indicating the intensity of reaction between a sample and the reagent in each of the sample wells; a detector for detecting signal produced by the reaction in each of the sample wells over a period of time comprising a single CCD scanning device for optically scanning the plurality of samples simultaneously; and computerized controller for simultaneously coordinating the pipettor, the excitation source, and the detector.

摘要翻译： 一种自动化检测系统包括用于对多个测试样品进行测定的机器人流体处理系统; 用于在进行测定时对分析进行成像的检测系统; 以及用于控制检测系统的操作并用于收集和分析成像数据的计算机控制，数据采集和数据分析系统。 该自动化检测系统包括移液器，用于同时将预定量的试剂分配到含有荧光染料或其它指示剂部分的多个样品中的每一个; 激发源，用于同时激发荧光染料的荧光反应，表明每个样品孔中的样品和试剂之间的反应强度; 检测器，用于在一段时间内检测每个样品阱中的反应产生的信号，包括用于同时光学扫描多个样品的单个CCD扫描装置; 和计算机控制器，用于同时协调移液器，激发源和检测器。