High speed digital imaging microscope
    2.
    发明授权
    High speed digital imaging microscope 失效
    高速数字成像显微镜

    公开(公告)号:US5233197A

    公开(公告)日:1993-08-03

    申请号:US729978

    申请日:1991-07-15

    IPC分类号: G01N21/64 G02B21/16

    摘要: A high speed fluorescent emission imaging digital microscope has an Argon-Ion UV laser illumination source and a high speed optical filtering wheel in the illumination path to alternately select between two UV illumination wavelengths to produce fluorescent emission images at each UV wavelength. A dichroic reflector directs emission images along an image path and an galvanometer rotatable mirror exposes each image on a predetermined area of a CCD. A computer controls the rotatable mirror in response to exposure timing information from the filtering wheel. An objective lens is focused on the specimen for producing an image from a particular focal plane of the specimen. The objective lens is mounted on a counterweighted piezoelectric objective translator for changing the focal plane of the specimen image in response to control signals from the electronic processor.

    摘要翻译: 高速荧光发射成像数字显微镜在照明路径中具有氩离子UV激光照明源和高速光学滤光轮,以在两个UV照射波长之间交替选择以在每个UV波长处产生荧光发射图像。 二向色反射器沿着图像路径引导发射图像,并且电流计可旋转镜将每个图像暴露在CCD的预定区域上。 计算机响应于来自过滤轮的曝光定时信息来控制可旋转镜。 物镜聚焦在样本上,用于从样本的特定焦平面生成图像。 物镜安装在配重的压电物镜转换器上,用于响应于来自电子处理器的控制信号改变样本图像的焦平面。