公开(公告)号：US5641457A

公开(公告)日：1997-06-24

申请号：US428693

申请日：1995-04-25

申请人： Michael H. Vardanega ,  Raymond Swan ,  John Joubran ,  David J. Medeiros ,  Edie Tichenor ,  Hugh Lewis

发明人： Michael H. Vardanega ,  Raymond Swan ,  John Joubran ,  David J. Medeiros ,  Edie Tichenor ,  Hugh Lewis

IPC分类号： B01L1/04 ,  G01N15/14 ,  G01N35/00 ,  G01N27/00

CPC分类号： G01N15/1404 ,  B01L1/04 ,  G01N2015/149 ,  G01N2035/00277

摘要： An apparatus and process for measuring particles that prevents the particles from being contaminated during the measuring and optional sorting process wherein a flow chamber or region is mechanically isolated from a sterile enclosure which surrounds the flow chamber. The present invention in one embodiment provides an apparatus comprising of a droplet generator, a charging system, a deflecting system, a sterilization system, a light source, a detector, a cooling system and a vacuum system. The droplet generator and the charging system and the flow chamber associated with the droplet generator operate within an illumination frame where the particles (e.g. biological cells) are actually measured. In order to prevent the biological cells from being contaminated, the illumination frame is located within an isolation chamber which provides an environment that can be sterilized before and after the actual measuring and sorting of the cells, but the illumination frame is mechanically isolated from the isolation chamber. The sterilization system, located outside the isolation chamber sterilizes the isolation chamber before cells are measured and sorted within the isolation chamber, and furthermore, ensures that the isolation chamber is maintained at a pressure above (or alternatively, below) atmospheric pressure during the measuring and sorting of the biological cells. The illumination frame, the light source and the detector are supported by a table to enable the light beam(s) from the light source to intersect the biological cells flowing through the illumination frame, thereby emitting flashes of light (or otherwise effecting the light beam) which are detected by the detector.

摘要翻译： 用于测量在测量和任选的分选过程中防止颗粒被污染的颗粒的装置和方法，其中流动室或区域与围绕流动室的无菌外壳机械隔离。 本发明在一个实施例中提供了一种包括液滴发生器，充电系统，偏转系统，灭菌系统，光源，检测器，冷却系统和真空系统的装置。 液滴发生器和与液滴发生器相关联的充气系统和流动室在其中实际测量颗粒（例如生物细胞）的照明框架内操作。 为了防止生物细胞被污染，照明框架位于隔离室内，该隔离室提供在实际测量和分选细胞之前和之后可以消毒的环境，但是照明框架与隔离件机械隔离 房间。 位于隔离室外部的灭菌系统在隔离室内测量和分选细胞之前对隔离室进行消毒，此外，确保隔离室在测量过程中保持在大气压（或者低于）大气压力以下， 生物细胞的分选。 照明框架，光源和检测器由桌子支撑，以使得来自光源的光束能够与流过照明框架的生物细胞相交，从而发​​出闪光（或以其他方式影响光束 ），由检测器检测。

公开(公告)号：US5776781A

公开(公告)日：1998-07-07

申请号：US803439

申请日：1997-02-20

申请人： Michael H. Vardanega ,  Raymond Swan ,  John Joubran ,  David J. Medeiros ,  Edie Tichenor ,  Hugh Lewis

发明人： Michael H. Vardanega ,  Raymond Swan ,  John Joubran ,  David J. Medeiros ,  Edie Tichenor ,  Hugh Lewis

IPC分类号： B01L1/04 ,  G01N15/14 ,  G01N35/00 ,  G01N29/00

CPC分类号： G01N15/1404 ,  B01L1/04 ,  G01N2015/149 ,  G01N2035/00277

摘要： An apparatus and process for measuring particles that prevents the particles from being contaminated during the measuring and optional sorting process wherein a flow chamber or region is mechanically isolated from a sterile enclosure which surrounds the flow chamber. The present invention in one embodiment provides an apparatus comprising of a droplet generator, a charging system, a deflecting system, a sterilization system, a light source, a detector, a cooling system and a vacuum system. The droplet generator and the charging system and the flow chamber associated with the droplet generator operate within an illumination frame where the particles (e.g. biological cells) are actually measured. In order to prevent the biological cells from being contaminated, the illumination frame is located within an isolation chamber which provides an environment that can be sterilized before and after the actual measuring and sorting of the cells, but the illumination frame is mechanically isolated from the isolation chamber. The sterilization system, located outside the isolation chamber sterilizes the isolation chamber before cells are measured and sorted within the isolation chamber, and furthermore, ensures that the isolation chamber is maintained at a pressure above (or alternatively, below) atmospheric pressure during the measuring and sorting of the biological cells. The illumination frame, the light source and the detector are supported by a table to enable the light beam(s) from the light source to intersect the biological cells flowing through the illumination frame, thereby emitting flashes of light (or otherwise effecting the light beam) which are detected by the detector.

摘要翻译： 用于测量在测量和任选的分选过程中防止颗粒被污染的颗粒的装置和方法，其中流动室或区域与围绕流动室的无菌外壳机械隔离。 本发明在一个实施例中提供了一种包括液滴发生器，充电系统，偏转系统，灭菌系统，光源，检测器，冷却系统和真空系统的装置。 液滴发生器和与液滴发生器相关联的充气系统和流动室在其中实际测量颗粒（例如生物细胞）的照明框架内操作。 为了防止生物细胞被污染，照明框架位于隔离室内，该隔离室提供在实际测量和分选细胞之前和之后可以消毒的环境，但是照明框架与隔离件机械隔离 房间。 位于隔离室外部的灭菌系统在隔离室内测量和分选细胞之前对隔离室进行消毒，此外，确保隔离室在测量过程中保持在大气压（或者低于）大气压力以下， 生物细胞的分选。 照明框架，光源和检测器由桌子支撑，以使得来自光源的光束能够与流过照明框架的生物细胞相交，从而发​​出闪光（或以其他方式影响光束 ），由检测器检测。