公开(公告)号：US06258577B1

公开(公告)日：2001-07-10

申请号：US09119666

申请日：1998-07-21

申请人： Raymond Paul Goodrich, Jr. ,  Frank Corbin, III ,  Edward C. Wood, Jr.

发明人： Raymond Paul Goodrich, Jr. ,  Frank Corbin, III ,  Edward C. Wood, Jr.

IPC分类号： C12N1300

CPC分类号： A61L2/0082 ,  A61L2/0011 ,  A61L2/0047 ,  A61L2/0052 ,  A61L2/08 ,  A61L2/10 ,  A61L2/23 ,  A61L2202/22 ,  A61M1/3683 ,  C12N7/04

摘要： Methods and apparatuses are provided for inactivation of microorganisms in a fluid containing blood or blood products and comprising biologically active proteins. The method includes the steps of adding an effective, non-toxic amount of an endogenous photosensitizer to the fluid; exposing the fluid to photoradiation sufficient to activate the endogenous photosensitizer; and allowing the activated endogenous photosensitizer to interfere with nucleic acid present in microorganisms in the fluid so that the microorganisms are inactivated. Isoalloxazines and K- and L-vitamins are among the preferred photosensitizers. Systems and apparatuses are also provided for decontamination of such fluids using photosensitizers which include means for adding photosensitizer to the fluid; a photopermeable container for exposing the fluid to an amount of photoradiation sufficient to activate the photosensitizer; and a photoradiation source, preferably comprising a light guide, for providing sufficient photoradiation of a type and amount selected to activate the photosensitizer. Means for controlling photosensitizer concentration in the fluid and rate of fluid flow through the container, including computer processors may be included in such systems.

摘要翻译： 提供了用于灭活含有血液或血液制品并含有生物活性蛋白质的流体中的微生物的方法和装置。 该方法包括将有效的无毒量的内源性光敏剂添加到流体中的步骤; 使流体暴露于足以激活内源性光敏剂的光照射; 并允许活化的内源性光敏剂干扰流体中存在于微生物中的核酸，使得微生物失活。 优选的光敏剂是异环氧嗪和K-和L-维生素。 还提供了系统和装置，用于使用光敏剂对这种流体进行净化，其包括将光敏剂添加到流体中的装置; 用于将流体暴露于足以激活光敏剂的光辐射量的光透过容器; 以及优选地包括光导的光引发源，用于提供所选择的激活光敏剂的类型和量的足够的光照射。 用于控制流体中光敏剂浓度和通过容器（包括计算机处理器）的流体流速的方法可以包括在这样的系统中。

公开(公告)号：US06277337B1

公开(公告)日：2001-08-21

申请号：US09357188

申请日：1999-07-20

申请人： Raymond Paul Goodrich, Jr. ,  Dennis Hlavinka ,  Frank Corbin, III ,  Edward C. Wood, Jr.

发明人： Raymond Paul Goodrich, Jr. ,  Dennis Hlavinka ,  Frank Corbin, III ,  Edward C. Wood, Jr.

IPC分类号： B01J1908

CPC分类号： A61L2/08 ,  A61L2/0011 ,  A61L2/0047 ,  A61L2/0052 ,  A61L2/0082 ,  A61L2/10 ,  A61L2/23 ,  A61L2202/22 ,  A61M1/3683 ,  C12N7/04

摘要： Methods and apparatuses are provided for inactivation of microorganisms in fluids or on surfaces. Preferably the fluids contain blood or blood products and comprise biologically active proteins. Preferred methods include the steps of adding an effective, non-toxic amount of an endogenous photosensitizer to a fluid and exposing the fluid to photoradiation sufficient to activate the endogenous photosensitizer whereby microorganisms are inactivated. Other fluids, including juices, water and the like, may also be decontaminated by these methods as may surfaces of foods, animal carcasses, wounds, food preparation surfaces and bathing and washing vessel surfaces. Alloxazines and K- and L-vitamins are among the preferred photosensitizers. Systems and apparatuses for flow-through and batch processes are also provided for decontamination of such fluids using photosensitizers.

摘要翻译： 提供了用于灭活流体或表面上的微生物的方法和装置。 优选地，流体含有血液或血液制品并且包含生物活性蛋白质。 优选的方法包括将有效的无毒量的内源性光敏剂加入到流体中并使流体暴露于足以激活内源性光敏剂从而使微生物灭活的步骤。 其他流体，包括果汁，水等也可以通过这些方法，如食品，动物屠体，伤口，食物制备表面和洗浴和洗涤容器表面的表面进行去污染。 Alloxazines和K-和L-维生素是优选的光敏剂之一。 还提供用于流通和间歇方法的系统和装置，用于使用光敏剂对这种流体进行去污染。