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公开(公告)号:US20070026010A1
公开(公告)日:2007-02-01
申请号:US10549064
申请日:2004-03-11
IPC分类号: A61K39/102 , C07H21/04 , C12N15/74 , C12N1/21 , C07K14/285
CPC分类号: A61K39/092 , A61K39/385 , A61K2039/6068 , C07K1/16 , C07K14/3156
摘要: The present invention relates to a method for purifying bacterial cytolysins such as pneumococcal pneumolysin. A single chromatography step produces excellent purification of the cytolysin by binding soluble aggregated cytolysin to a hydrophobic interaction chromatography material in the presence of detergent and high salt.
摘要翻译: 本发明涉及一种纯化细菌细胞溶质蛋白如肺炎球菌肺炎杆菌素的方法。 单一色谱步骤通过在洗涤剂和高盐存在下将可溶性聚集的细胞溶解素结合到疏水相互作用层析材料上,产生细胞溶素的极好的纯化。