公开(公告)号：US20050118143A1

公开(公告)日：2005-06-02

申请号：US10695600

申请日：2003-10-28

Applicant: Dennis Steindler ,  Eric Laywell ,  Valery Kukekov ,  L. Thomas

Inventor： Dennis Steindler ,  Eric Laywell ,  Valery Kukekov ,  L. Thomas

IPC: C12N5/0797 ,  A61K48/00 ,  C12N5/08

CPC classification number: C12N5/0623 ,  C12N2500/25 ,  C12N2500/44 ,  C12N2500/84 ,  C12N2501/11 ,  C12N2501/115

Abstract: Using a novel culture approach, previously unknown populations of neural progenitor cells have been found within an adult mammalian brain. By limiting cell-cell contact, dissociated adult brain yields at least two types of cell aggregates. These aggregates or clones of stem/precursor cells can be generated from adult brain tissue with significantly long postmortem intervals Both neurons and glia arise from stem/precursor cells of these cultures, and the cells can survive transplantation to the adult mammalian brain.

Abstract translation: 使用新的培养方法，已经在成年哺乳动物脑内发现了以前未知的神经祖细胞群。 通过限制细胞 - 细胞接触，解离的成年脑产生至少两种类型的细胞聚集体。 茎/前体细胞的这些聚集体或克隆可以从成年脑组织产生，具有显着长的死后间隔。这些培养物的茎/前体细胞都产生神经元和神经胶质细胞，并且细胞可以通过移植到成年哺乳动物脑中。

公开(公告)号：US20050031538A1

公开(公告)日：2005-02-10

申请号：US10912472

申请日：2004-08-05

Applicant: Dennis Steindler ,  Eric Laywell ,  Tong Zheng

Inventor： Dennis Steindler ,  Eric Laywell ,  Tong Zheng

IPC: A61K49/00 ,  G01N33/50 ,  G01N33/53 ,  G01N33/567

CPC classification number: G01N33/5058 ,  A61K49/0008 ,  G01N33/5008 ,  G01N33/5014 ,  G01N33/5023 ,  G01N33/5073 ,  G01N2510/00

Abstract: Methods and assay systems for analyzing effects of chemical and cellular agents on brain cell neurogenesis in vivo, comprising administering an agent to a test animal and determining responses of cells of brain marrow tissues, including irradiated brain marrow tissue depleted of neurogenic stems cells, and cells in brain marrow-derived neurospheres cultured in vitro.

Abstract translation: 用于分析化学和细胞试剂对体内脑细胞神经发生的影响的方法和测定系统，包括向测试动物施用药剂并测定脑骨髓组织细胞的应答，包括耗尽神经源性干细胞的照射的骨髓组织和细胞 在体外培养的脑髓来源的神经球中。