公开(公告)号：US4937200A

公开(公告)日：1990-06-26

申请号：US32501

申请日：1987-03-31

申请人： Toshiaki Kumazawa ,  Makoto Nakamura ,  Eriko Kogure

发明人： Toshiaki Kumazawa ,  Makoto Nakamura ,  Eriko Kogure

IPC分类号： G01N33/50 ,  G01N33/543

CPC分类号： G01N33/543

摘要： A method of effecting an immunoassay using affinity chromatography which a sample is injected into a reusable column charged with antibody applied on a solid support, an eluent is injected into the column to elute antigen bound with the antibody on the solid support, and an amount of eluted antigen is measured. In order to mitigate the influence of carry-overs of successive samples, blank values are corrected such that they include the carry-overs of previously measured samples, while elution time periods are kept constant. In one embodiment, the elution time periods are corrected such that blank values for respective samples are made constant.

摘要翻译： 使用亲和层析进行免疫测定的方法，其将样品注入装有施加在固体支持体上的抗体的可重复使用的柱中，将洗脱液注入柱中以洗脱固体支持物上与抗体结合的抗原， 测定洗脱的抗原。 为了减轻连续样品的遗留影响，校正空白值，使得它们包括先前测量的样品的残留，而洗脱时间段保持恒定。 在一个实施方案中，校正洗脱时间周期，使得各个样品的空白值保持恒定。

公开(公告)号：US4855229A

公开(公告)日：1989-08-08

申请号：US936320

申请日：1986-12-01

申请人： Eriko Kogure ,  Makoto Nakamura ,  Toshiaki Kumazawa

发明人： Eriko Kogure ,  Makoto Nakamura ,  Toshiaki Kumazawa

IPC分类号： C12Q1/28

CPC分类号： C12Q1/28 ,  C12Q2326/20 ,  Y10S435/81

摘要： A measurement method of peroxidase activity is based on reaction of o-phenylenediamine and hydrogen peroxide in the presence of peroxidase. This method includes preparing and storing a first reagent containing o-phenylenediamine and having a first pH value higher than a second pH value at which the reaction is conducted, and preparing and storing a second reagent having a buffering capability larger than the first reagent. At the time of measurement, the stored first and second reagents are mixed together with a sample containing peroxidase, and a predetermined amount of hydrogen peroxide to provide a reaction mixture having the second pH value. The reaction is conducted in the reaction mixture, thereby producing 2,2'-diamino-azobenzene, and the absorbance increase at the maximum absorption wavelength of the produced 2,2'-diamino-azobenzene is measured.

摘要翻译： 过氧化物酶活性的测定方法是基于邻苯二胺和过氧化氢在过氧化物酶存在下的反应。 该方法包括制备和储存含有邻苯二胺的第一试剂，其具有高于进行反应的第二pH值的第一pH值，以及制备和储存具有大于第一试剂的缓冲能力的第二试剂。 在测量时，将储存的第一和第二试剂与含有过氧化物酶的样品和预定量的过氧化氢混合在一起以提供具有第二pH值的反应混合物。 在反应混合物中进行反应，由此生成2,2'-二氨基偶氮苯，测定所生成的2,2'-二氨基偶氮苯的最大吸收波长处的吸光度增加。