公开(公告)号：US4752570A

公开(公告)日：1988-06-21

申请号：US774044

申请日：1985-09-09

申请人： Rainer Wehner ,  Helmut Lenz

发明人： Rainer Wehner ,  Helmut Lenz

IPC分类号： C12Q1/28 ,  G01N33/535 ,  C12N9/99 ,  C12Q1/30

CPC分类号： C12Q1/28 ,  C12Q2326/20 ,  C12Q2326/30

摘要： The present invention provides a process for the determination of peroxidase by the addition of a peroxide and of a chromogen and measurement of the color resulting from the oxidation of the chromogen, the color formation being stopped after a definite time by the addition of a stop agent, wherein catalase is used as stop agent.

摘要翻译： 本发明提供了通过添加过氧化物和色原来测定过氧化物酶的方法以及由色原体氧化产生的颜色的测定，通过添加停止剂在一定时间后停止着色形成 ，其中过氧化氢酶用作终止剂。

公开(公告)号：US4683197A

公开(公告)日：1987-07-28

申请号：US730605

申请日：1985-05-06

申请人： Harald Gallati

发明人： Harald Gallati

IPC分类号： G01N33/53 ,  C12Q1/28 ,  G01N33/48 ,  G01N33/50 ,  G01N33/543 ,  G01N33/72 ,  G01N33/546

CPC分类号： G01N33/543 ,  C12Q1/28 ,  G01N33/725 ,  C12Q2326/20

摘要： A method for the detection of occult human blood in human stool samples by immunologically determining after performing the known peroxidase procedure, a human component abnormally present in the stool.

摘要翻译： 通过在执行已知的过氧化物酶程序之后通过免疫测定在粪便中异常存在的人类成分来检测人粪便样品中的隐藏人血液的方法。

公开(公告)号：US4855229A

公开(公告)日：1989-08-08

申请号：US936320

申请日：1986-12-01

申请人： Eriko Kogure ,  Makoto Nakamura ,  Toshiaki Kumazawa

发明人： Eriko Kogure ,  Makoto Nakamura ,  Toshiaki Kumazawa

IPC分类号： C12Q1/28

CPC分类号： C12Q1/28 ,  C12Q2326/20 ,  Y10S435/81

摘要： A measurement method of peroxidase activity is based on reaction of o-phenylenediamine and hydrogen peroxide in the presence of peroxidase. This method includes preparing and storing a first reagent containing o-phenylenediamine and having a first pH value higher than a second pH value at which the reaction is conducted, and preparing and storing a second reagent having a buffering capability larger than the first reagent. At the time of measurement, the stored first and second reagents are mixed together with a sample containing peroxidase, and a predetermined amount of hydrogen peroxide to provide a reaction mixture having the second pH value. The reaction is conducted in the reaction mixture, thereby producing 2,2'-diamino-azobenzene, and the absorbance increase at the maximum absorption wavelength of the produced 2,2'-diamino-azobenzene is measured.

摘要翻译： 过氧化物酶活性的测定方法是基于邻苯二胺和过氧化氢在过氧化物酶存在下的反应。 该方法包括制备和储存含有邻苯二胺的第一试剂，其具有高于进行反应的第二pH值的第一pH值，以及制备和储存具有大于第一试剂的缓冲能力的第二试剂。 在测量时，将储存的第一和第二试剂与含有过氧化物酶的样品和预定量的过氧化氢混合在一起以提供具有第二pH值的反应混合物。 在反应混合物中进行反应，由此生成2,2'-二氨基偶氮苯，测定所生成的2,2'-二氨基偶氮苯的最大吸收波长处的吸光度增加。

公开(公告)号：US07208284B2

公开(公告)日：2007-04-24

申请号：US11003416

申请日：2004-12-06

申请人： Kenji Takagi

发明人： Kenji Takagi

IPC分类号： G01N33/00

CPC分类号： C12Q1/26 ,  C12Q2326/20 ,  G01N33/57488

摘要： A method for measuring polyamines in erythrocytes in which a polyamine oxidase having a substrate specificity to spermine and spermidine or a polyamine oxidase having a substrate specificity to spermine only is used, an eluate in erythrocytes separated and purified from blood is reacted with these enzymes, and hydrogen peroxide formed is determined with a highly sensitive chromogen1 such as a diphenylamine-based chromogen. A method for measuring amounts of polyamines in erythrocytes easily with high precision. The method is effective for diagnosis of certain disease conditions or physiological conditions or the like.

摘要翻译： 用于测量红细胞中多胺的方法，其中使用具有对精胺和亚精胺具有底物特异性的多胺氧化酶或仅对精胺具有底物特异性的多胺氧化酶，从血液中分离和纯化的红细胞中的洗脱液与这些酶反应， 形成的过氧化氢用高度敏感的色原1（例如基于二苯胺的色原体）测定。 用于以高精度容易地测量红细胞中多胺的量的方法。 该方法对某些疾病状态或生理条件等的诊断是有效的。

公开(公告)号：US5776703A

公开(公告)日：1998-07-07

申请号：US580397

申请日：1995-03-02

申请人： Seymon Bystryak

发明人： Seymon Bystryak

IPC分类号： C12Q1/28 ,  G01N20060101 ,  G01N33/53 ,  G01N33/535 ,  C12Q1/26

CPC分类号： C12Q1/28 ,  G01N33/535 ,  C12Q2326/20 ,  G01N2333/155 ,  G01N2333/16 ,  G01N2333/62 ,  G01N2333/805

摘要： There is provided an assay of enhanced sensitivity for the qualitative and quantitative determination of haptens. These may be assayed entities such as antigens, antibodies, a wide variety of biologically active proteins. The novel assay of increased sensitivity is based on the formation of a complex of the entity to be measured with another entity, tagging this conjugate with a oxidizing entity, reacting the resultant entity with a redox substrate to form a product which is photosensitive and irradiating same thus enhancing the sensitivity of the assay. Colored or fluorescent products give highly sensitive results. According to a preferred embodiment the oxidized entity is reacted with a non-ionic detergent, enhancing the resulting fluorescence.

摘要翻译： 提供了用于定性和定量测定半抗原的增强灵敏度的测定。 这些可以是测定的实体，例如抗原，抗体，多种生物活性蛋白质。 增加灵敏度的新型测定是基于与另一实体的待测实体的复合物的形成，将该缀合物与氧化实体标记，使得到的实体与氧化还原底物反应以形成光敏并照射的产物 从而提高了测定的灵敏度。 彩色或荧光产品给予高度敏感的结果。 根据优选的实施方案，氧化实体与非离子型洗涤剂反应，增强所得到的荧光。

公开(公告)号：US20050277166A1

公开(公告)日：2005-12-15

申请号：US11003416

申请日：2004-12-06

申请人： Kenji Takagi

发明人： Kenji Takagi

IPC分类号： G01N21/78 ,  C12Q1/02 ,  C12Q1/26 ,  G01N33/574

CPC分类号： C12Q1/26 ,  C12Q2326/20 ,  G01N33/57488

摘要： A method for measuring polyamines in erythrocytes in which a polyamine oxidase having a substrate specificity to spermine and spermidine or a polyamine oxidase having a substrate specificity to spermine only is used, an eluate in erythrocytes separated and purified from blood is reacted with these enzymes, and hydrogen peroxide formed is determined with a highly sensitive chromogen such as a diphenylamine-based chromogen. There is provided a method for measuring amounts of polyamines in erythrocytes easily with high precision. The method is effective for diagnosis of certain disease conditions or physiological conditions or the like of subjects.

摘要翻译： 用于测量红细胞中多胺的方法，其中使用具有对精胺和亚精胺具有底物特异性的多胺氧化酶或仅对精胺具有底物特异性的多胺氧化酶，从血液中分离和纯化的红细胞中的洗脱液与这些酶反应， 用高灵敏度的色原如二苯胺类色原来测定形成的过氧化氢。 提供了以高精度容易地测量红细胞中多胺量的方法。 该方法对于某些疾病状况或受试者的生理条件等的诊断是有效的。

公开(公告)号：US5079150A

公开(公告)日：1992-01-07

申请号：US272360

申请日：1988-11-17

申请人： Daniel B. Wagner ,  Glen P. Vonk ,  Randy A. Hoke

发明人： Daniel B. Wagner ,  Glen P. Vonk ,  Randy A. Hoke

IPC分类号： G01N33/52 ,  C12Q1/28 ,  G01N33/535 ,  G01N33/543

CPC分类号： C12Q1/28 ,  C12Q2326/20 ,  Y10S435/805 ,  Y10S435/97

摘要： A new class of peroxidase substrates consists of o-diaminobenzenes having a substituent in the 4 position. The new substrates may be used in immunoassays in which a peroxidase is the label, or in assays for the peroxidase itself. Immunoassays using other enzyme labels which cause formation of hydrogen peroxide may be followed using the substrate of the invention by adding the substrate and a peroxidase to the assay fluid and detecting color formed as a result of oxidation of the substrate by the formed peroxide.

公开(公告)号：US5017475A

公开(公告)日：1991-05-21

申请号：US89605

申请日：1987-08-25

申请人： Richard A. Harte ,  Stephen H. Mastin

发明人： Richard A. Harte ,  Stephen H. Mastin

IPC分类号： C12Q1/28

CPC分类号： C12Q1/28 ,  C12Q2326/00 ,  C12Q2326/20

摘要： Methods are provided for the fluorescent detection of an analyte of interest wherein an amine-substituted, ortho-fused pyrazine fluorophore, with neither nitrogen of the pyrazine ring being fused or substituted, is produced by enzymatic oxidation of a fluorophore precursor substrate which comprises a nitrogen-substituted, cyclic compound. Also provided are novel fluorophore-labelled compounds.

摘要翻译： 提供了用于感兴趣分析物的荧光检测的方法，其中吡嗪环的氮均未被稠合或取代的胺取代的邻位稠合的吡嗪荧光团通过含有氮的荧光团前体基质的酶促氧化产生 取代的环状化合物。 还提供了新的荧光团标记的化合物。