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公开(公告)号:US5665586A
公开(公告)日:1997-09-09
申请号:US343428
申请日:1994-11-18
CPC分类号: C12N9/52 , Y10S435/896
摘要: According to the present invention, a novel protease derived from Streptomyces fradiae for cleaving the C-terminal of a glutamic acid residue in an amino acid sequence of a polypeptide and a production method for this protease from Streptomyces fradiae are provided, and the characteristics of this protease are made clear. Further, DNA sequences for encoding this protease and a prepropeptide are also made clear. The protease specific to glutamic acid can be used for a variety of purposes including analysis of a protein and cleavage of a peptide chain at a desired site in a fusion protein.
摘要翻译: PCT No.PCT / JP93 / 00592 Sec。 371日期:1994年11月18日 102(e)1994年11月18日PCT 1993年4月30日PCT PCT。 出版物WO93 / 23530 日期:1993年11月25日根据本发明,提供了一种用于切割多肽的氨基酸序列中的谷氨酸残基的C-末端的链霉菌(Streptomyces fradiae)的新型蛋白酶和来自链霉菌(Streptomyces fradiae)的该蛋白酶的制备方法, 并且使该蛋白酶的特征清楚。 此外,编码该蛋白酶和前肽的DNA序列也是清楚的。 对谷氨酸特异性的蛋白酶可用于多种目的,包括蛋白质分析和融合蛋白中所需位点处的肽链切割。
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公开(公告)号:US5459064A
公开(公告)日:1995-10-17
申请号:US35634
申请日:1993-03-23
申请人: Hiroshi Teraoka , Mikio Tamaki , Etsuo Nakamura , Masaru Shin , Nobuo Yoshida , Hiroshige Tsuzuki , Takashi Fujiwara , Koichi Matsumoto
发明人: Hiroshi Teraoka , Mikio Tamaki , Etsuo Nakamura , Masaru Shin , Nobuo Yoshida , Hiroshige Tsuzuki , Takashi Fujiwara , Koichi Matsumoto
IPC分类号: C12N1/21 , C12N9/48 , C12N9/56 , C12N15/09 , C12N15/57 , C12N15/75 , C12R1/10 , C12N15/11 , C12N15/52
CPC分类号: C12N9/54
摘要: A novel protease derived from Bacillus licheniformis is provided. The protease cleaves the peptide bonds at the carboxyl termini of glutamic acid residues in polypeptides. The protease contains an amino acid sequence from serine in the +1 position to glutamine in the +222 position of SEQ ID NO: 1.
摘要翻译: 提供了一种衍生自地衣芽孢杆菌的新型蛋白酶。 蛋白酶切割多肽中谷氨酸残基羧基末端的肽键。 蛋白酶含有从+1位的丝氨酸到SEQ ID NO:1的+222位的谷氨酰胺的氨基酸序列。
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