公开(公告)号：US20120305478A1

公开(公告)日：2012-12-06

申请号：US13516760

申请日：2010-07-29

申请人： Alan Gideon Werker ,  Simon Olof Harald Bengtsson ,  Fernando Morgan-Sagastume ,  Carl Anton Börje Karlsson ,  Elise Marie Blanchet

发明人： Alan Gideon Werker ,  Simon Olof Harald Bengtsson ,  Fernando Morgan-Sagastume ,  Carl Anton Börje Karlsson ,  Elise Marie Blanchet

IPC分类号： C02F3/12 ,  C02F3/34

CPC分类号： C12P7/625 ,  C02F1/24 ,  C02F3/1263 ,  C02F3/34 ,  C02F2203/004 ,  C02F2209/21 ,  C02F2209/22 ,  Y02W10/15 ,  Y02W10/45

摘要： A method or process is provided for treating wastewater and producing a polyhydroxyalkanote (PHA)-storing biomass. The method or process entails biologically treating wastewater and in one process a filamentous biomass is selected and caused to proliferate so as to dominate an activated sludge. The filamentous biomass is utilized to treat the wastewater and to remove contaminants therefrom. As a part of this process, there is provided an enhancement for PHA production potential in the said biomass. This entails enhancing the PHA production potential of the filamentous biomass by subjecting the biomass to alternating feast and famine conditions where under feast conditions more biodegradable organic substrate is available to the filamentous biomass than under famine conditions. In another process, wastewater is treated with an activated sludge. The wastewater is treated in a main stream and as a part of the process, the activated sludge and biomass contained therein is concentrated and directed to a side stream. In the side stream, at least a portion of the enhancement for PHA production potential in the biomass from the process is carried out. In one particular process, the activated sludge and the biomass contained therein is concentrated by a separator and the concentrated biomass is directed to a side stream and subjected to famine conditions.

摘要翻译： 提供了一种用于处理废水并生产聚羟基卡那酮（PHA）的生物质的方法或方法。 该方法或方法需要生物处理废水，并且在一个过程中，丝状生物质被选择并引起增殖以便主导活性污泥。 丝状生物质用于处理废水并从中除去污染物。 作为该过程的一部分，提供了在所述生物质中PHA生产潜力的增强。 这需要通过使生物质经历交替的盛宴和饥荒条件来提高丝状生物质的PHA生产潜力，其中在盛宴条件下，在饥荒条件下，丝状生物质可获得更可生物降解的有机底物。 在另一个过程中，废水用活性污泥处理。 废水在主流中进行处理，作为该方法的一部分，其中所含的活性污泥和生物质被浓缩并引导至侧流。 在侧流中，进行来自该方法的生物质中PHA生产潜力的增强的至少一部分。 在一个特定的方法中，活性污泥及其中所含的生物质通过分离器浓缩，浓缩的生物质被引导至侧流并经历饥荒条件。

公开(公告)号：US20150353967A1

公开(公告)日：2015-12-10

申请号：US14760217

申请日：2014-01-13

申请人： Alan Gideon WERKER ,  Fernando MORGAN-SAGASTUME ,  Lamija KARABEGOVIC ,  Simon Olof Harald BENGTSSON ,  Francesco VALENTINO ,  Mauro MAJONE ,  VEOLIA WATER SOLUTIONS & TECHNOLOGIES sUPPORT

发明人： Alan Gideon WERKER ,  Fernando MORGAN-SAGASTUME ,  Lamija KARABEGOVIC ,  Simon Olof Harald BENGTSSON ,  Francesco VALENTINO ,  Mauro MAJONE

IPC分类号： C12P7/62 ,  C02F3/12

CPC分类号： C12P7/625 ,  C02F3/006 ,  C02F3/12 ,  C02F11/02 ,  C02F2209/21 ,  C02F2305/06

摘要： This disclosure relates to methods for producing a PHA-rich-biomass from open mixed cultures. Mixed liquor containing biomass is directed into a fed-batch reactor. The reactor includes at least one biomass stimulating zone and at least one biomass maintenance zone. A feed containing RBCOD, bioavailable N, and bioavailable P is directed into the fed-batch reactor. The respiration rate of at least a portion of the biomass is intermittently and repeatedly stimulated in the stimulating zone by directing the feed into the fed-batch reactor and exposing the biomass to a relatively high concentration of RBCOD. The biomass is then transferred to the maintenance zone, where the biomass is exposed to a relatively low concentration of RBCOD. Thereafter, the biomass is circulated back and forth between the stimulating zone and the maintenance zone. Throughout the methods, the concentration of N and/or P relative to the RBCOD in the feed is controlled by controlling the ratio of N to RBCOD and/or the ration of P to RBCOD in the feed.

摘要翻译： 本公开涉及从开放混合培养物生产富含PHA的生物质的方法。 含有生物质的混合液被引入补料分批反应器。 反应器包括至少一个生物质刺激区和至少一个生物质维持区。 将含有RBCOD，生物可利用的N和生物可利用的P的饲料引入补料分批反应器。 通过将饲料引入补料分批反应器并将生物量暴露于较高浓度的RBCOD，在刺激区中间歇地和重复地刺激至少一部分生物质的呼吸速率。 然后将生物量转移到维护区，其中生物量暴露于较低浓度的RBCOD。 此后，生物质在刺激区和维护区之间来回循环。 在整个方法中，通过控制饲料中N与RBCOD的比例和/或P与RBCOD的比例来控制饲料中N和/或P相对于RBCOD的浓度。

公开(公告)号：US20170233271A1

公开(公告)日：2017-08-17

申请号：US15502052

申请日：2015-08-06

申请人： Alan Gideon WERKER ,  Simon Olof Harald BENGTSSON ,  John Markus HJORT ,  Fernando MORGAN-SAGASTUME ,  Mauro MAJONE ,  Francesco VALENTINO ,  VEOLIA WATER SOLUTIONS & TECHNOLOGIES SUPPORT ,  UNIVERSITY OF ROME

发明人： Alan Gideon Werker ,  Simon Olof Harald Bengtsson ,  John Markus Hjort ,  Fernando Morgan-Sagastume ,  Mauro Majone ,  Francesco Valentino

IPC分类号： C02F3/12 ,  C12P7/62

CPC分类号： C02F3/1205 ,  C02F3/1263 ,  C02F3/34 ,  C02F2203/004 ,  C02F2209/06 ,  C02F2209/08 ,  C02F2209/22 ,  C02F2305/06 ,  C12P7/625 ,  Y02W10/15

摘要： A process for producing PHA comprises obtaining biomass produced in the course of biologically treating a first wastewater source containing RBCOD. The biomass is to be exploited with a second wastewater source having a different RBCOD content from the first wastewater source in order to accumulate and thereby produce PHA. Before subjecting the biomass to a PHA accumulation process, the biomass PHA accumulation potential is enhanced via an acclimation process with the second wastewater source. During acclimation, the biomass is subjected to repeated feast-famine periods. During each feast period, the biomass is exposed to a fraction of the second wastewater source. The RBCOD uptake and/or biomass respiration rate is directly or indirectly measured during each feast period. The famine period is maintained for a period of time that is at least two times greater than the length of time of the proceeding feast period. After at least two feast-famine acclimation periods or after one or more measured parameters reveal an increased RBCOD relative uptake or respiration rate of the biomass during a subsequent feast period, the biomass is subjected to a PHA accumulation process using the second wastewater source.

公开(公告)号：US20130199997A1

公开(公告)日：2013-08-08

申请号：US13817021

申请日：2011-08-17

申请人： Alan Gideon Werker ,  Simon Olof Harald Bengtsson ,  Elise Marie Blanchet ,  Maria das Dores Tavares Gurgo e Cirne ,  Carl Anton Börje Karlsson ,  Fernando Morgan-Sagastume

发明人： Alan Gideon Werker ,  Simon Olof Harald Bengtsson ,  Elise Marie Blanchet ,  Maria das Dores Tavares Gurgo e Cirne ,  Carl Anton Börje Karlsson ,  Fernando Morgan-Sagastume

IPC分类号： C02F3/12

CPC分类号： C02F3/1221 ,  C02F3/12 ,  C02F3/1263 ,  C02F2203/004 ,  C02F2209/10 ,  C02F2209/21 ,  Y02W10/15 ,  Y02W10/45

摘要： A method of biologically treating wastewater and removing contaminants from the wastewater is disclosed. In the course of treating the wastewater, biomass is produced. In addition to removing contaminants from the wastewater, the process or method of the present invention entails enhancing the PHA accumulation potential of the biomass. Disclosed are a number of processes that are employed in a biological wastewater treatment system for enhancing PHA accumulation potential. For example, enhanced PHA accumulation potential is realized by exposing the biomass to feast and famine conditions and, after exposing the biomass to famine conditions, stimulating the biomass into a period of feast by exposing the biomass to feast conditions for a selected period of time by applying an average peak stimulating RBCOD feeding rate of greater than 5 mg-COD\L\MIN in combination with an average peak specific RBCOD feeding rate greater than 0.5 mg-COD\g-VSS\MIN. In another example, the PHA accumulation potential of the biomass is enhanced by subjecting the biomass to feast conditions that cause the biomass to reach a peak respiration rate that is at least 40% of the extant maximum respiration rate of the biomass. Other processes are discussed that can contribute to enhancing PHA accumulation potential of biomass.

摘要翻译： 公开了生物处理废水并从废水中去除污染物的方法。 在处理废水的过程中，生产生物质。 除了从废水中除去污染物外，本发明的方法或方法需要提高生物质的PHA积累潜力。 公开了用于提高PHA积累潜力的生物废水处理系统中的许多方法。 例如，增强的PHA积累潜力是通过将生物量暴露于饥饿和饥荒条件下实现的，并且在将生物质暴露于饥荒条件之后，通过将生物质暴露于选定时间段的盛宴条件来将生物质刺激到一段盛宴期间 应用平均峰值刺激RBCOD摄入率大于5 mg-COD \ L \ MIN与平均峰值特异性RBCOD摄入率大于0.5 mg-COD \ g-VSS \ MIN。 在另一个实例中，生物质的PHA积累潜力通过使生物质经历使得生物质达到至少40％的生物量最大呼吸速率的峰值呼吸速率的条件来增强。 讨论了有助于提高生物质PHA积累潜力的其他过程。